The study of enzyme mechanisms by a combination of cosolvent, low-temperature and high-pressure techniques

scientific article published on November 1, 1979

The study of enzyme mechanisms by a combination of cosolvent, low-temperature and high-pressure techniques is …
instance of (P31):
review articleQ7318358
scholarly articleQ13442814

External links are
P356DOI10.1017/S0033583500002808
P953full work available at URLhttps://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0033583500002808
P698PubMed publication ID95127

P2093author name stringP. Douzou
P2860cites workSome factors in the interpretation of protein denaturationQ28202994
Bacterial bioluminescenceQ28264654
Low temperature trapping method for cytochrome oxidase oxygen intermediatesQ33257491
Peptide chain termination. VII. The ribosomal and release factor requirements for peptide releaseQ33692561
Temperature-dependent X-ray diffraction as a probe of protein structural dynamicsQ34214441
Ribosome-catalyzed peptidyl transfer: substrate specificity at the P-siteQ34655271
Spectral properties of an oxygenated luciferase-flavin intermediate isolated by low-temperature chromatographyQ34747025
A thermodynamic model of regulation: modulation of redox equilibria in camphor monoxygenaseQ34995870
Effect of a low-molecular-weight DNA binding protein, H1 factor, on the in vitro transcription of the lactose operon in Escherichia coliQ35067993
Functional intermediates in reaction of cytochrome oxidase with oxygenQ35077346
Activation volumes in enzymic catalysis: their sources and modification by low-molecular-weight solutesQ35085264
Protein hydration changes during catalysis: a new mechanism of enzymic rate-enhancement and ion activation/inhibition of catalysisQ35087304
Sedimentation behavior of chemically reacting systemsQ35971434
Sequential translation of trinucleotide codons for peptide bond formation, translocation, and terminationQ35979278
The influence of the medium dielectric strength upon trypsin kineticsQ36373312
Pressure-Induced Dissociation of Sedimenting Ribosomes: Effect on Sedimentation PatternsQ37482977
Structure of the oxygen adduct intermediate in the bacterial luciferase reaction: C nuclear magnetic resonance determinationQ37595739
Protein crystallography at sub-zero temperatures: Lysozyme-substrate complexes in cooled mixed solventsQ38360216
Practical potentiometric determinations of proton activity in hydro organic solvents at subzero temperaturesQ39105968
Formation of stable crystalline enzyme–substrate intermediates at sub-zero temperaturesQ39109803
Ionization Dependence of Camphor Binding and Spin Conversion of the Complex between Cytochrome P-450 and Camphor. Kinetic and Static Studies at Sub-zero TemperaturesQ39159326
Cryoenzymology: The Use of Fluid Solvent Mixtures at Subzero Temperatures for the Study of Biochemical ReactionsQ39741097
Protein Fractionation At Subzero TemperaturesQ39779303
Interactions between the quaternary structure of the globin and the spin state of the heme in ferric mixed spin derivatives of hemoglobinQ40169743
Protein crystallography at sub-zero temperatures: Cryo-protective mother liquors for protein crystalsQ40343844
The -chymotrypsin-catalyzed hydrolysis of N-acetyl-L-tryptophan p-nitrophenyl ester in dimethyl sulfoxide at subzero temperaturesQ42234453
Crystal structure of elastase-substrate complex at -- 55 degrees CQ43977044
Effect of dimethyl sulfoxide on the interaction of proflavine with alpha-chymotrypsinQ47894390
Pressure denaturation of metmyoglobinQ47910694
Fluorescence and bioluminescence of bacterial luciferase intermediatesQ50959125
Trypsin-organic solvent interaction. Simultaneous operation of competitive inhibition and dielectric effectQ52979186
The sub-zero temperature chromatographic isolation of transient intermediates of a multi-step cycle: preparation of the substrate-free oxy-ferrous cytochrome P450Q54694447
Testing cosolvent cryoenzymology on multi-enzyme systemsQ66946841
Conformational changes and local events at the AMP site of glycogen phosphorylase b: a fluorescence temperature-jump relaxation studyQ67402394
Stabilization of a phosphorylase b active conformation by hydrophobic solventsQ67416583
The sub-zero temperature chromatographic isolation of transient intermediates of a multi-step cycle: purification of the substrate-bound oxy-ferrous cytochrome P450Q67864653
Ribosomal activities dependent on elongation factors T and G. Effects of methanolQ68688791
Dielectric constant of mixed solvents used for a low temperature biochemistryQ68803266
Transcription in vitro using mixtures of ethylene glycol and waterQ69305927
Thermodynamics of protein denaturation. Effect of pressure on the denaturation on ribonuclease AQ71429503
Molecular kinetics of muscle adenosine triphosphatase. II. Solvent and structural effectsQ73172964
The influence of pressure on the rates of biological reactionsQ75670648
THE EFFECT OF APROTIC DIPOLAR ORGANIC SOLVENTS ON THE KINETICS OF ALPHA-CHYMOTRYPSIN-CATALYZED HYDROLYSESQ76593128
Substrate and antibiotic binding sites at the peptidyl transferase centre of E. coli ribosomesQ77922695
Effects of Pressure and Temperature on the Catalytic and Regulatory Properties of Muscle Pyruvate Kinase from an Off-Shore Benthic FishQ114632927
P433issue4
P407language of work or nameEnglishQ1860
P921main subjectcoldQ270952
biophysicsQ7100
P304page(s)78
P577publication date1979-11-01
P1433published inQuarterly Reviews of BiophysicsQ2361372
P1476titleThe study of enzyme mechanisms by a combination of cosolvent, low-temperature and high-pressure techniques
P478volume12

Reverse relations

Q59663016[24] Triggering methods in crystallographic enzyme kineticscites workP2860

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