A single mutation in the activation site of bovine trypsinogen enhances its accumulation in the fermentation broth of the yeast Pichia pastoris

scientific article

A single mutation in the activation site of bovine trypsinogen enhances its accumulation in the fermentation broth of the yeast Pichia pastoris is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1128/AEM.69.2.1108-1113.2003
P932PMC publication ID143679
P698PubMed publication ID12571036
P5875ResearchGate publication ID10914421

P2093author name stringFranc Pattus
Dominique Desplancq
Jean-François Lefèvre
Alain A Vertès
Charles L Hershberger
José Hanquier
Laurence Baroche
Marc Ebtinger
Yannick Sorlet
P2860cites workIdentification and expression of the cDNA-encoding human mesotrypsin(ogen), an isoform of trypsin with inhibitor resistanceQ24312765
Structure of bovine trypsinogen at 1.9 Å resolutionQ27729075
Hydrophobic interactions control zymogen activation in the trypsin family of serine proteasesQ30423680
Generation of rabbit monoclonal antibody fragments from a combinatorial phage display library and their production in the yeast Pichia pastorisQ32060532
Mechanisms of zymogen activationQ34577303
Engineered turns of a recombinant antibody improve its in vivo foldingQ36706337
Expression of rat chymotrypsinogen in yeast: a study on the structural and functional significance of the chymotrypsinogen propeptideQ36799585
Use of the glyceraldehyde-3-phosphate dehydrogenase promoter for production of functional mammalian membrane transport proteins in the yeast Pichia pastorisQ36898657
Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiaeQ37560151
Transformation of Schizosaccharomyces pombe by electroporationQ40530528
Recent advances in the expression of foreign genes in Pichia pastorisQ40533559
Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastorisQ40666201
Expression and characterization of glycosylated and catalytically active recombinant human alpha-galactosidase A produced in Pichia pastorisQ40840927
Production and isolation of the recombinant N-lobe of human serum transferrin from the methylotrophic yeast Pichia pastorisQ42523416
Glyceraldehyde-3-phosphate dehydrogenase-derived expression cassettes for constitutive synthesis of heterologous proteinsQ46432824
Development of an efficient method for generating and screening active trypsin and trypsin variantsQ46746659
A versatile set of vectors for constitutive and regulated gene expression in Pichia pastorisQ47796942
Production of a recombinant bovine enterokinase catalytic subunit in the methylotrophic yeast Pichia pastorisQ47941032
Mapping the pro-region of carboxypeptidase B by protein engineering. Cloning, overexpression, and mutagenesis of the porcine proenzymeQ47951186
Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoterQ48053752
High-level secretion of two antibody single chain Fv fragments by Pichia pastoris.Q48053879
Autolysis of beta-Trypsin. Influence of Calcium Ions and HeatQ53930504
High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the geneQ67527996
Protein glycosylation in yeastQ68949058
The denaturation of trypsinQ70472117
Efficient expression and secretion of recombinant alpha amylase in Pichia pastoris using two different signal sequencesQ71676448
Production and purification of recombinant hirudin expressed in the methylotrophic yeast Pichia pastorisQ71859628
Purification and Preliminary Characterization of dDAP, a Novel Dipeptidylaminopeptidase from Dictyostelium discoideumQ72030206
The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast Pichia pastorisQ72738167
Functional expression of bovine opsin in the methylotrophic yeast Pichia pastorisQ73405606
High-level expression of bovine beta-lactoglobulin in Pichia pastoris and characterization of its physical propertiesQ74350346
Variation in N-linked oligosaccharide structures on heterologous proteins secreted by the methylotrophic yeast Pichia pastorisQ77471895
Overview of N- and O-linked oligosaccharide structures found in various yeast speciesQ77761193
Action of the kallikreins on synthetic ester substratesQ79102714
P433issue2
P407language of work or nameEnglishQ1860
P921main subjectPichia pastorisQ148697
P304page(s)1108-1113
P577publication date2003-02-01
P1433published inApplied and Environmental MicrobiologyQ4781593
P1476titleA single mutation in the activation site of bovine trypsinogen enhances its accumulation in the fermentation broth of the yeast Pichia pastoris
P478volume69

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cites work (P2860)
Q36404285Biochemical Characterization of An Arginine-Specific Alkaline Trypsin from Bacillus licheniformis
Q64244102Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast
Q33509255Characterization of a serine protease homologous to house dust mite group 3 allergens from the scabies mite Sarcoptes scabiei
Q84655484Functional expression of trypsin from Streptomyces griseus by Pichia pastoris
Q47263159Identification of a precursor processing protease from the spider Cupiennius salei essential for venom neurotoxin maturation
Q39063721Improved Production of Active Streptomyces griseus Trypsin with a Novel Auto-Catalyzed Strategy
Q36333101Manipulating corynebacteria, from individual genes to chromosomes
Q89004617Production and cleavage of a fusion protein of porcine trypsinogen and enhanced green fluorescent protein (EGFP) in Pichia pastoris
Q53295186Production of functional recombinant bovine trypsin in transgenic rice cell suspension cultures
Q43270373Recombinant shrimp (Litopenaeus vannamei) trypsinogen production in Pichia pastoris.
Q41903520Variable production windows for porcine trypsinogen employing synthetic inducible promoter variants in Pichia pastoris.

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