Multiple mechanisms contribute to osmotic inducibility of proU operon expression in Escherichia coli: demonstration of two osmoresponsive promoters and of a negative regulatory element within the first structural gene

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Multiple mechanisms contribute to osmotic inducibility of proU operon expression in Escherichia coli: demonstration of two osmoresponsive promoters and of a negative regulatory element within the first structural gene is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1128/JB.173.23.7481-7490.1991
P932PMC publication ID212513
P698PubMed publication ID1938945

P2093author name stringGowrishankar J
Rajkumari K
Dattananda CS
P2860cites workPhysiological and genetic responses of bacteria to osmotic stressQ24594026
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencingQ27860941
Histone-like protein H1 (H-NS), DNA supercoiling, and gene expression in bacteriaQ28242053
A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coliQ28280711
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmidQ29614535
In vitro reconstitution of osmoregulated expression of proU of Escherichia coliQ33836838
Superhelical torsion in cellular DNA responds directly to environmental and genetic factorsQ33866962
Some simple computational methods to improve the folding of large RNAsQ35532878
Osmotic signal transduction to proU is independent of DNA supercoiling in Escherichia coliQ36125847
Bacterial DNA supercoiling and [ATP]/[ADP] ratio: changes associated with salt shockQ36147307
Rapid site-specific DNA inversion in Escherichia coli mutants lacking the histonelike protein H-NSQ36148365
Nucleotide sequence of the osmoregulatory proU operon of Escherichia coliQ36175605
The problems of eukaryotic and prokaryotic DNA packaging and in vivo conformation posed by superhelix density heterogeneityQ36220323
cis-acting proteinsQ36249200
Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposonsQ36300204
Linkage map of Escherichia coli K-12, edition 8Q37056213
Biology of bacterial deoxyribonucleic acid topoisomerasesQ37066515
Unusual DNA structuresQ39625578
Characterization of mutations affecting the osmoregulated proU promoter of Escherichia coli and identification of 5' sequences required for high-level expressionQ39938469
Formation of (dA-dT)n cruciforms in Escherichia coli cells under different environmental conditionsQ39941327
Osmoregulation in Escherichia coli: complementation analysis and gene-protein relationships in the proU locusQ39948554
Nucleotide sequence of the transcriptional control region of the osmotically regulated proU operon of Salmonella typhimurium and identification of the 5' endpoint of the proU mRNA.Q39950073
Osmotic regulation of transcription: induction of the proU betaine transport gene is dependent on accumulation of intracellular potassiumQ39961568
Molecular cloning of an osmoregulatory locus in Escherichia coli: increased proU gene dosage results in enhanced osmotoleranceQ39961796
Molecular analysis of the regulatory region of the Escherichia coli K-12 tyrB geneQ39962617
Identification of osmoresponsive genes in Escherichia coli: evidence for participation of potassium and proline transport systems in osmoregulation.Q39964115
Transition of a cloned d(AT)n-d(AT)n tract to a cruciform in vivo.Q40470729
Ptac-85, an E. coli vector for expression of non-fusion proteinsQ40561518
Molecular characterization of the proU loci of Salmonella typhimurium and Escherichia coli encoding osmoregulated glycine betaine transport systemsQ42645491
Osmotic control of proU transcription is mediated through direct action of potassium glutamate on the transcription complexQ46093136
Systematic characterization of curved DNA segments randomly cloned from Escherichia coli and their functional significanceQ48227092
Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coliQ48395208
A novel, non-invasive promoter probe vector: cloning of the osmoregulated proU promoter of Escherichia coli K12.Q50194304
The osmZ (bglY) gene encodes the DNA-binding protein H-NS (H1a), a component of the Escherichia coli K12 nucleoid.Q54707912
Transcription generates positively and negatively supercoiled domains in the template.Q54748648
Variability of the intracellular ionic environment of Escherichia coli. Differences between in vitro and in vivo effects of ion concentrations on protein-DNA interactions and gene expression.Q54764867
Promoter recognition by Escherichia coli RNA polymerase. Effects of substitutions in the spacer DNA separating the -10 and -35 regions.Q54776694
Transcriptional silencing and thermoregulation of gene expression in Escherichia coliQ59097717
Topoisomerase mutants and physiological conditions control supercoiling and Z-DNA formation in vivoQ67912532
SPXX, a frequent sequence motif in gene regulatory proteinsQ69209195
Characterization of the osmoregulated Escherichia coli proU promoter and identification of ProV as a membrane-associated proteinQ69268132
In an Escherichia coli coupled transcription-translation system, expression of the osmoregulated gene proU is stimulated at elevated potassium concentrations and by an extract from cells grown at high osmolalityQ69370091
P433issue23
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
P304page(s)7481-7490
P577publication date1991-12-01
P1433published inJournal of BacteriologyQ478419
P1476titleMultiple mechanisms contribute to osmotic inducibility of proU operon expression in Escherichia coli: demonstration of two osmoresponsive promoters and of a negative regulatory element within the first structural gene
P478volume173

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