Detection of sub-picogram quantities of specific DNA sequences on blot hybridization with biotinylated probes.

scientific article

Detection of sub-picogram quantities of specific DNA sequences on blot hybridization with biotinylated probes. is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1093/NAR/13.22.8083
P932PMC publication ID322111
P698PubMed publication ID2933635
P5875ResearchGate publication ID20221894

P50authorKenneth FlemingQ46098555
James O'Donnell McGeeQ112412991
P2093author name stringChan VT
P2860cites workHybridization of denatured RNA and small DNA fragments transferred to nitrocelluloseQ24611198
Immunological method for mapping genes on Drosophila polytene chromosomesQ24630484
Detection of specific sequences among DNA fragments separated by gel electrophoresisQ25939003
Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) proceduresQ26778382
Isolation of a transforming sequence from a human bladder carcinoma cell lineQ28264736
A new method of in situ hybridizationQ28283758
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfateQ29618203
A membrane-filter technique for the detection of complementary DNAQ29618219
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probesQ30450044
A colorimetric method for DNA hybridizationQ35275953
Application of the avidin-biotin method of gene enrichment to the isolation of long double-stranded DNA containing specific gene sequencesQ36184913
In situ hybridization at the electron microscope level: hybrid detection by autoradiography and colloidal goldQ36209523
Chemically modified nucleic acids as immunodetectable probes in hybridization experimentsQ36269845
Actin gene expression visualized in chicken muscle tissue culture by using in situ hybridization with a biotinated nucleotide analogQ36323529
Cloning and characterization of DNA sequences surrounding the human gamma-, delta-, and beta-globin genesQ36398828
Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells.Q37196528
Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blotsQ37616420
Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotidesQ40567617
Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotinQ40567920
A complex of single-strand binding protein and M13 DNA as hybridization probeQ40568761
Polynucleotide-histone H1 complexes as probes for blot hybridizationQ41455447
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probesQ64380989
High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistryQ70289767
Comparisons using 35S- and 32P-labeled DNA for hybridization on nitrocellulose filtersQ71124286
The use of the avidin-biotin complex as a tool in molecular biologyQ71247261
Biotin-labeled oligonucleotides: enzymatic synthesis and use as hybridization probesQ71425730
2-Acetylaminofluorene-modified probes for the indirect hybridocytochemical detection of specific nucleic acid sequencesQ72735909
Nucleic acid guanine: reaction with the carcinogen N-acetoxy-2-acetylaminofluoreneQ72798785
P433issue22
P407language of work or nameEnglishQ1860
P304page(s)8083-8091
P577publication date1985-11-01
P1433published inNucleic Acids ResearchQ135122
P1476titleDetection of sub-picogram quantities of specific DNA sequences on blot hybridization with biotinylated probes.
P478volume13

Reverse relations

cites work (P2860)
Q40390097A homogeneous nucleic acid hybridization assay based on strand displacement
Q69426333A native, affinity-based protein blot for the analysis of streptavidin heterogeneity: consequences for the specificity of streptavidin mediated binding assays
Q54688211A nested PCR followed by magnetic separation of amplified fragments for detection of Escherichia coli Shiga-like toxin genes
Q40450928A new biotinylating system for DNA using biotin aminocaproyl hydrazide and glutaraldehyde
Q37109046Alpha-1 antitrypsin gene exon use in stimulated lymphocytes
Q64380039Biotinylated DNA probes: sensitivity and applications
Q36439931Biotinylated nucleic acid hybridization probes for potato virus detection
Q61703975Chromosomal localization of genes by scanning electron microscopy usingin situ hybridization with biotinylated probes: Y chromosome repetitive sequences
Q58844746Detection and Classification ofTrypanosoma cruziby DNA Hybridization with Nonradioactive Probes
Q33838126Detection of low copy human papilloma virus DNA and mRNA in routine paraffin sections of cervix by non-isotopic in situ hybridisation
Q48127565Detection of proopiomelanocortin mRNA by in situ hybridization, using a biotinylated oligodeoxynucleotide probe and avidin-alkaline phosphatase histochemistry
Q36632645Diagnostic deoxyribonucleic acid probes for infectious diseases
Q36002758Direct visualization of single copy genes on banded metaphase chromosomes by nonisotopicin situhybridization
Q64453005Discrimination of closely homologous HPV types by nonisotopicin situ hybridization: definition and derivation of tissue melting temperatures
Q55504570Dr Burns and colleagues reply.
Q42828943Enzymatic detection systems for non-isotopic in situ hybridization using biotinylated cDNA probes
Q60361458Extrachromosomal plasmid-like DNA in the obligate parasitic fungus Erysiphe graminis f. sp. hordei
Q35833791Glomerular expression of interleukin-1 receptor antagonist and interleukin-1 beta genes in antibody-mediated glomerulonephritis
Q33674101High resolution gene mapping of the human alpha globin locus
Q39578026Human and viral gene detection in routine paraffin embedded tissue by in situ hybridisation with biotinylated probes: viral localisation in herpes encephalitis
Q36079862Human papillomavirus status in the prediction of high-grade cervical intraepithelial neoplasia in patients with persistent low-grade cervical cytological abnormalities.
Q67952475Identification of flow-sorted chromosomes by G-banding and in situ hybridization
Q37142705In situ human papillomavirus (HPV) genotyping of cervical intraepithelial neoplasia in South African and British patients: evidence for putative HPV integration in vivo
Q68228772In situ hybridization: alkaline phosphatase visualization of biotinylated probes in cryostat and paraffin sections
Q42843841In vitro amplification of hepatitis B virus sequences from liver tumour DNA and from paraffin wax embedded tissues using the polymerase chain reaction
Q41961063Interphase cytogenetics using biotin and digoxigenin labelled probes I: relative sensitivity of both reporter molecules for detection of HPV16 in CaSki cells.
Q37145475Interphase cytogenetics using biotin and digoxigenin labelled probes: III. Increased sensitivity and flexibility for detecting HPV in cervical biopsy specimens and cell lines
Q33481935Model system for optimising mRNA non-isotopic in situ hybridisation: riboprobe detection of lysozyme mRNA in archival gut biopsy specimens
Q33932761Molecular techniques in the diagnosis of human infectious diseases.
Q40814398Monitoring impurities in biopharmaceuticals produced by recombinant technology
Q33839108Monoclonal antibody EBM/11: high cellular specificity for human macrophages
Q33839413Non-isotopic detection of in situ nucleic acid in cervix: an updated protocol
Q68266791Non-radioactive detection of immunoglobulin and T cell receptor gene rearrangement in acute lymphoblastic leukaemia
Q79147666Non-radioactive techniques for the labelling of nucleic acids
Q39962316Physical map of the Rhodobacter sphaeroides bacteriophage phi RsG1 genome and location of the prophage on the host chromosome
Q40407059Polyethylene tube: an alternative support for synthesis and purification of biotin-labeled single-stranded DNA probes
Q67866619Production of digoxigenin-labelled parvovirus DNA probe by PCR
Q40521828Protein-DNA conjugates produced by UV irradiation and their use as probes for hybridization
Q40555398Rapid production of vector-free biotinylated probes using the polymerase chain reaction
Q59171307Solid phase synthesis of 5'-phosphate labelled polynucleotides
Q44771858The application of alkaline phosphatase labeled HBV probe in serum detection
Q40448273The signal intensity on Southern blots developed by nonisotopic methods is linear with time and quantity of DNA.
Q68376996Tissue-plasminogen activator RNA detected in megakaryocytes by in situ hybridization and biotinylated probe
Q37409635Use of nonisotopic M13 probes for genetic analysis: application to HLA class II loci
Q68992355[Technic of nucleic acid hybridization and its significance for diagnostic problems]