Cell-Free Hepatitis B Virus Capsid Assembly Dependent on the Core Protein C-Terminal Domain and Regulated by Phosphorylation

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Cell-Free Hepatitis B Virus Capsid Assembly Dependent on the Core Protein C-Terminal Domain and Regulated by Phosphorylation is …
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scholarly articleQ13442814

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P356DOI10.1128/JVI.00394-16
P932PMC publication ID4886785
P698PubMed publication ID27076641

P50authorChristian VoitenleitnerQ43801119
P2093author name stringJianming Hu
Kuancheng Liu
Laurie Ludgate
William E Delaney
Nicholas Streck
Laurie Luckenbaugh
Stacey Eng
P2860cites workThe crystal structure of the human hepatitis B virus capsidQ27618957
Hepatitis core antigen produced in Escherichia coli: subunit composition, conformational analysis, and in vitro capsid assemblyQ30193506
Hepatitis B virus nucleocapsid assembly: primary structure requirements in the core proteinQ30195835
Nucleic acid chaperone activity associated with the arginine-rich domain of human hepatitis B virus core protein.Q30357029
Testing the balanced electrostatic interaction hypothesis of hepatitis B virus DNA synthesis by using an in vivo charge rebalance approachQ33648943
Serine phosphoacceptor sites within the core protein of hepatitis B virus contribute to genome replication pleiotropicallyQ33831725
Reverse transcription-associated dephosphorylation of hepadnavirus nucleocapsidsQ33863206
Cys residues of the hepatitis B virus capsid protein are not essential for the assembly of viral core particles but can influence their stabilityQ33935637
Full-length hepatitis B virus core protein packages viral and heterologous RNA with similarly high levels of cooperativityQ33966563
Production and function of the cytoplasmic deproteinized relaxed circular DNA of hepadnavirusesQ39782358
Multiple functions of capsid protein phosphorylation in duck hepatitis B virus replicationQ40038150
Effect of core protein phosphorylation by protein kinase C on encapsidation of RNA within core particles of hepatitis B virus.Q40042957
The arginine-rich domain of the hepatitis B virus core protein is required for pregenome encapsidation and productive viral positive-strand DNA synthesis but not for virus assemblyQ40066232
RNA- and DNA-binding activities in hepatitis B virus capsid protein: a model for their roles in viral replicationQ40309060
Stability and morphology comparisons of self-assembled virus-like particles from wild-type and mutant human hepatitis B virus capsid proteinsQ40344665
Inhibition of hepatitis B virus replication by drug-induced depletion of nucleocapsidsQ40606293
Hepadnaviral assembly is initiated by polymerase binding to the encapsidation signal in the viral RNA genomeQ41536446
The interface between hepatitis B virus capsid proteins affects self-assembly, pregenomic RNA packaging, and reverse transcriptionQ41610650
Roles of the three major phosphorylation sites of hepatitis B virus core protein in viral replicationQ41676475
Physicochemical analysis of the hepatitis B virus core antigen produced by a baculovirus expression vectorQ42072659
Expression of hepatitis B virus core and precore antigens in insect cells and characterization of a core-associated kinase activityQ42073033
Conserved cysteines of the hepatitis B virus core protein are not required for assembly of replication-competent core particles nor for their envelopmentQ43587474
Replication of the genome of a hepatitis B-like virus by reverse transcription of an RNA intermediateQ44294970
The structure of hepadnaviral core antigens. Identification of free thiols and determination of the disulfide bonding patternQ44909338
Differentiation of core gene products of the hepatitis B virus in infected liver tissue using monoclonal antibodiesQ45760074
Full and empty particles of hepatitis B virus in hepatocytes from patients with HBsAg-positive chronic active hepatitis.Q45793958
Topological analysis of the hepatitis B virus core particle by cysteine-cysteine cross-linking.Q45865507
Hepatitis B virus infectionQ88131866
Secretion of genome-free hepatitis B virus--single strand blocking model for virion morphogenesis of para-retrovirusQ34037662
Phosphoacceptors threonine 162 and serines 170 and 178 within the carboxyl-terminal RRRS/T motif of the hepatitis B virus core protein make multiple contributions to hepatitis B virus replicationQ34059140
A kinase chaperones hepatitis B virus capsid assembly and captures capsid dynamics in vitroQ34082677
The morphogenic linker peptide of HBV capsid protein forms a mobile array on the interior surfaceQ34085995
Phosphorylation state-dependent interactions of hepadnavirus core protein with host factorsQ34117726
C-terminal substitution of HBV core proteins with those from DHBV reveals that arginine-rich 167RRRSQSPRR175 domain is critical for HBV replicationQ34387651
Structural organization of pregenomic RNA and the carboxy-terminal domain of the capsid protein of hepatitis B virusQ34426465
Regulation of multiple stages of hepadnavirus replication by the carboxyl-terminal domain of viral core protein in transQ35076767
Regulation of hepadnavirus reverse transcription by dynamic nucleocapsid phosphorylation.Q35635378
Hepadnavirus Genome Replication and PersistenceQ35795545
The Dual Role of an ESCRT-0 Component HGS in HBV Transcription and Naked Capsid SecretionQ35797289
Phosphorylation and nuclear localization of the hepatitis B virus core protein: significance of serine in the three repeated SPRRR motifsQ35832241
Genome-free hepatitis B virion levels in patient sera as a potential marker to monitor response to antiviral therapyQ35846564
Formation of hepatitis B virus covalently closed circular DNA: removal of genome-linked proteinQ35857166
Deamination-independent inhibition of hepatitis B virus reverse transcription by APOBEC3G.Q35857453
A recombinant hepatitis B core antigen polypeptide with the protamine-like domain deleted self-assembles into capsid particles but fails to bind nucleic acidsQ35895863
Hepatitis B Virus Core Protein Phosphorylation Sites Affect Capsid Stability and Transient Exposure of the C-terminal DomainQ36301368
Cyclin-dependent kinase 2 phosphorylates s/t-p sites in the hepadnavirus core protein C-terminal domain and is incorporated into viral capsidsQ36363961
A micromolar pool of antigenically distinct precursors is required to initiate cooperative assembly of hepatitis B virus capsids in Xenopus oocytes.Q36639300
Characterization of hepatitis B virus capsid particle assembly in Xenopus oocytesQ36697538
RNA-protein interactions in hepadnavirus reverse transcriptionQ36727768
To build a virus on a nucleic acid substrate.Q36742699
Reverse transcriptase- and RNA packaging signal-dependent incorporation of APOBEC3G into hepatitis B virus nucleocapsidsQ36747707
Characterization of the major duck hepatitis B virus core particle proteinQ36781843
Phosphorylation in the carboxyl-terminal domain of the capsid protein of hepatitis B virus: evaluation with a monoclonal antibodyQ36821141
Maturation-associated destabilization of hepatitis B virus nucleocapsidQ37252759
Hepatitis B virus capsid particles are assembled from core-protein dimer precursorsQ37264041
Testing an electrostatic interaction hypothesis of hepatitis B virus capsid stability by using an in vitro capsid disassembly/reassembly systemQ37365388
Requirement of heat shock protein 90 for human hepatitis B virus reverse transcriptase functionQ37596493
Hsp90 is required for the activity of a hepatitis B virus reverse transcriptaseQ37729269
Core protein: A pleiotropic keystone in the HBV lifecycleQ38541052
Exposure of RNA templates and encapsidation of spliced viral RNA are influenced by the arginine-rich domain of human hepatitis B virus core antigen (HBcAg 165-173).Q39164496
Genetically altering the thermodynamics and kinetics of hepatitis B virus capsid assembly has profound effects on virus replication in cell cultureQ39217491
Core protein phosphorylation modulates pregenomic RNA encapsidation to different extents in human and duck hepatitis B virusesQ39591153
P433issue12
P407language of work or nameEnglishQ1860
P921main subjectHepatitis B virusQ6844
phosphorylationQ242736
P304page(s)5830-5844
P577publication date2016-04-13
P1433published inJournal of VirologyQ1251128
P1476titleCell-Free Hepatitis B Virus Capsid Assembly Dependent on the Core Protein C-Terminal Domain and Regulated by Phosphorylation
P478volume90

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cites work (P2860)
Q57445698Assembly Properties of Hepatitis B Virus Core Protein Mutants Correlate with Their Resistance to Assembly-Directed Antivirals
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Q92802270Combining Cell-Free Protein Synthesis and NMR Into a Tool to Study Capsid Assembly Modulation
Q54216735Common and Distinct Capsid and Surface Protein Requirements for Secretion of Complete and Genome-free Hepatitis B Virions.
Q37727852Complete and Incomplete Hepatitis B Virus Particles: Formation, Function, and Application
Q96304040Conservation and variability of hepatitis B core at different chronic hepatitis stages
Q40178518Discovery and Mechanistic Study of Benzamide Derivatives That Modulate Hepatitis B Virus Capsid Assembly.
Q64947767Discovery of Novel Hepatitis B Virus Nucleocapsid Assembly Inhibitors.
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Q37498902HBV maintains electrostatic homeostasis by modulating negative charges from phosphoserine and encapsidated nucleic acids
Q47601905Hepatitis B core protein as a therapeutic target.
Q52579748Hepatitis B virus core protein dephosphorylation occurs during pregenomic RNA encapsidation.
Q61443712Hepatitis B virus core protein phosphorylation: Identification of the SRPK1 target sites and impact of their occupancy on RNA binding and capsid structure
Q55277503Host-regulated Hepatitis B Virus Capsid Assembly in a Mammalian Cell-free System.
Q59352472Identification of Compounds Targeting Hepatitis B Virus Core Protein Dimerization through a Split Luciferase Complementation Assay
Q40150658Identification of Intermediate in Hepatitis B Virus CCC DNA Formation and Sensitive and Selective CCC DNA Detection
Q55393682Multiple roles of core protein linker in hepatitis B virus replication.
Q38695872PGC1α Transcriptional Adaptor Function Governs Hepatitis B Virus Replication by Controlling HBcAg/p21 Protein-Mediated Capsid Formation.
Q59137720Post-translational Modification Control of HBV Biological Processes
Q89823737Prolyl Isomerase Pin1 Regulates the Stability of Hepatitis B Virus Core Protein
Q92382035Pyrimidotriazine derivatives as selective inhibitors of HBV capsid assembly
Q90743474Role of Hepatitis B virus capsid phosphorylation in nucleocapsid disassembly and covalently closed circular DNA formation
Q89967522Synthesis and Assembly of Hepatitis B Virus-Like Particles in a Pichia pastoris Cell-Free System
Q47355500The diverse functions of the hepatitis B core/capsid protein (HBc) in the viral life cycle: Implications for the development of HBc-targeting antivirals.

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