HIV-1 reverse transcriptase specifically interacts with the anticodon domain of its cognate primer tRNA.

scientific article published on November 1989

HIV-1 reverse transcriptase specifically interacts with the anticodon domain of its cognate primer tRNA. is …
instance of (P31):
scholarly articleQ13442814

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P932PMC publication ID401457
P698PubMed publication ID2479543

P50authorFrançoise Barré-SinoussiQ103844
P2093author name stringKeith G
Darlix JL
Barat C
Nugeyre MT
Schatz O
Grüninger-Leitch F
Lullien V
LeGrice SF
P2860cites workCharacterization of ribosomal frameshifting in HIV-1 gag-pol expressionQ46573916
Interactions of retroviral structural proteins with single-stranded nucleic acids.Q52598487
trans-Diamminedichloroplatinum(II), a reversible RNA-protein cross-linking agent. Application to the ribosome and to an aminoacyl-tRNA synthetase/tRNA complex.Q54762499
Parallel association of Fos and Jun leucine zippers juxtaposes DNA binding domainsQ57963273
Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for Acquired Immune Deficiency Syndrome (AIDS)Q22242256
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteinsQ27860587
“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AQ28131712
Nucleotide sequence of the AIDS virus, LAVQ28286313
Nucleotide Sequence of Three Isoaccepting Lysine tRNAs from Rabbit Liver and SV40-Transformed Mouse FibroblastsQ28322712
Site-specific mutagenesis of AIDS virus reverse transcriptaseQ28338101
Conserved cysteine and histidine residues of the avian myeloblastosis virus nucleocapsid protein are essential for viral replication but are not "zinc-binding fingers".Q33650421
Point mutants of Moloney murine leukemia virus that fail to package viral RNA: evidence for specific RNA recognition by a "zinc finger-like" protein sequenceQ33669533
Low-molecular-weight RNAs and initiation of RNA-directed DNA synthesis in avian reticuloendotheliosis virusQ33914438
Rous sarcoma virus nucleic acid-binding protein p12 is necessary for viral 70S RNA dimer formation and packagingQ33927402
Structural characterization of reverse transcriptase and endonuclease polypeptides of the acquired immunodeficiency syndrome retrovirusQ33929366
AIDS virus reverse transcriptase defined by high level expression in Escherichia coliQ33929729
Association of viral reverse transcriptase with an enzyme degrading the RNA moiety of RNA-DNA hybridsQ34206884
Identification and characterization of HIV-specific RNase H by monoclonal antibodyQ34403789
Specific binding of tryptophan transfer RNA to avian myeloblastosis virus RNA-dependent DNA polymerase (reverse transcriptase)Q35083339
Viral DNA synthesized in vitro by avian retrovirus particles permeabilized with melittin. I. Kinetics of synthesis and size of minus- and plus-strand transcriptsQ35235192
Interactions between avian myeloblastosis reverse transcriptase and tRNATrp. Mapping of complexed tRNA with chemicals and nucleasesQ35267456
Amino acid sequence homology in gag region of reverse transcribing elements and the coat protein gene of cauliflower mosaic virusQ36088934
Mapping adenines, guanines, and pyrimidines in RNAQ36183946
Native ribonucleoprotein is an efficient transcriptional complex of avian myeloblastosis virusQ36359086
Reverse transcriptase as the major determinant for selective packaging of tRNA's into Avian sarcoma virus particlesQ36508415
Mutations in Rous sarcoma virus nucleocapsid protein p12 (NC): deletions of Cys-His boxesQ36872467
Biochemical and immunological analysis of human immunodeficiency virus gag gene products p17 and p24.Q36875767
Moloney murine sarcoma virions synthesize full-genome-length double-stranded DNA in vitroQ37336368
Computer analysis of retroviral pol genes: assignment of enzymatic functions to specific sequences and homologies with nonviral enzymesQ37402708
Human immunodeficiency virus 1 reverse transcriptase. Template binding, processivity, strand displacement synthesis, and template switchingQ38345021
The isolation of avian viral RNA and polypeptidesQ39543566
Processing protease and reverse transcriptase from human immunodeficiency virus type I polyprotein in Escherichia coliQ40127549
Standardized and simplified nomenclature for proteins common to all retrovirusesQ40127929
Small finger protein of avian and murine retroviruses has nucleic acid annealing activity and positions the replication primer tRNA onto genomic RNA.Q41107385
Immunological and chemical analysis of P6, the carboxyl-terminal fragment of HIV P15.Q44219513
Ordered interstrand and intrastrand DNA transfer during reverse transcriptionQ45058628
Studies on the mechanism of human immunodeficiency virus reverse transcriptase. Steady-state kinetics, processivity, and polynucleotide inhibition.Q45276674
It is Rous sarcoma virus protein P12 and not P19 that binds tightly to Rous sarcoma virus RNA.Q45796797
Photochemical cross-linking studies on the interactions of avian myeloblastosis virus reverse transcriptase with primer tRNATrp and TTP.Q45805697
Fine structure of human immunodeficiency virus (HIV) and immunolocalization of structural proteinsQ45830218
Genetic variability of the AIDS virus: nucleotide sequence analysis of two isolates from African patientsQ45848343
Leucine repeats and an adjacent DNA binding domain mediate the formation of functional cFos-cJun heterodimersQ46196460
Purification of tRNATrp, tRNAVal, and partial purification of tRNAIle and tRNAMfet from beef liverQ46561607
P433issue11
P407language of work or nameEnglishQ1860
P304page(s)3279-3285
P577publication date1989-11-01
P1433published inThe EMBO JournalQ1278554
P1476titleHIV-1 reverse transcriptase specifically interacts with the anticodon domain of its cognate primer tRNA.
P478volume8

Reverse relations

cites work (P2860)
Q430894936th international symposium on retroviral nucleocapsid
Q36332732A mimic of HIV-1 nucleocapsid protein impairs reverse transcription and displays antiviral activity
Q27939540A single zinc finger optimizes the DNA interactions of the nucleocapsid protein of the yeast retrotransposon Ty3.
Q36626314A specific orientation of RNA secondary structures is required for initiation of reverse transcription.
Q33897049A succession of mechanisms stimulate efficient reconstituted HIV-1 minus strand strong stop DNA transfer
Q37701346Amino acid signature enables proteins to recognize modified tRNA.
Q36973475Aminoacyl-tRNA synthetase complexes: molecular multitasking revealed
Q40792308An RNA chaperone activity of non-specific RNA binding proteins in hammerhead ribozyme catalysis
Q40394716Analysis of the nucleic acid annealing activities of nucleocapsid protein from HIV-1.
Q36687225Analytical study of avian reticuloendotheliosis virus dimeric RNA generated in vivo and in vitro
Q33749767Arbovirus detection in insect vectors by rapid, high-throughput pyrosequencing
Q36645128Basic amino acids flanking the zinc finger of Moloney murine leukemia virus nucleocapsid protein NCp10 are critical for virus infectivity
Q34091700Biophysical characterization of DNA binding from single molecule force measurements
Q35128110Characterization of a nucleocapsid-like region and of two distinct primer tRNALys,2 binding sites in the endogenous retrovirus Gypsy
Q33850680Cleavage of tRNA within the mature tRNA sequence by the catalytic RNA of RNase P: implication for the formation of the primer tRNA fragment for reverse transcription in copia retrovirus-like particles
Q34751283Cleavage of the HIV replication primer tRNALys,3 in human cells expressing bacterial anticodon nuclease
Q38022377Comparative nucleic acid chaperone properties of the nucleocapsid protein NCp7 and Tat protein of HIV-1.
Q38349052Complementation of a primer binding site-impaired murine leukemia virus-derived retroviral vector by a genetically engineered tRNA-like primer.
Q38362410Construction of a type 1 human immunodeficiency virus that maintains a primer binding site complementary to tRNA(His).
Q39922639DNA condensation by the nucleocapsid protein of HIV-1: a mechanism ensuring DNA protection
Q36797561Deletions in the tRNA(Lys) primer-binding site of human immunodeficiency virus type 1 identify essential regions for reverse transcription
Q28289627Determination of the structure of the nucleocapsid protein NCp7 from the human immunodeficiency virus type 1 by 1H NMR
Q33937534Domain structure of the human immunodeficiency virus reverse transcriptase
Q36810310Double-stranded RNA-dependent RNase activity associated with human immunodeficiency virus type 1 reverse transcriptase
Q43109836Effect of Mg(2+) and Na(+) on the nucleic acid chaperone activity of HIV-1 nucleocapsid protein: implications for reverse transcription
Q39879855Effect of mutations in the nucleocapsid protein (NCp7) upon Pr160(gag-pol) and tRNA(Lys) incorporation into human immunodeficiency virus type 1.
Q35854248Effects of 3'-deoxynucleoside 5'-triphosphate concentrations on chain termination by nucleoside analogs during human immunodeficiency virus type 1 reverse transcription of minus-strand strong-stop DNA
Q36635791Effects of alterations of primer-binding site sequences on human immunodeficiency virus type 1 replication
Q35864780Effects of modifying the tRNA(3Lys) anticodon on the initiation of human immunodeficiency virus type 1 reverse transcription
Q40393707Efficient extension of a misaligned tRNA-primer during replication of the HIV-1 retrovirus
Q34589376Extended interactions between the primer tRNAi(Met) and genomic RNA of the yeast Ty1 retrotransposon.
Q33677123Functional expansion of human tRNA synthetases achieved by structural inventions
Q26746070HIV Genome-Wide Protein Associations: a Review of 30 Years of Research
Q34557590HIV-1 RT-associated ribonuclease H displays both endonuclease and 3′----5′ exonuclease activity
Q35865776Human immunodeficiency virus Type 1 nucleocapsid protein (NCp7) directs specific initiation of minus-strand DNA synthesis primed by human tRNA(Lys3) in vitro: studies of viral RNA molecules mutated in regions that flank the primer binding site
Q45790185Human immunodeficiency virus nucleocapsid protein stimulates strand transfer from internal regions of heteropolymeric RNA templates
Q35847385Human immunodeficiency virus type 1 can use different tRNAs as primers for reverse transcription but selectively maintains a primer binding site complementary to tRNA(3Lys)
Q38309738Human immunodeficiency virus type-1 reverse transcription can be inhibited in vitro by oligonucleotides that target both natural and synthetic tRNA primers
Q27676632Human tRNALys3UUU Is Pre-Structured by Natural Modifications for Cognate and Wobble Codon Binding through Keto–Enol Tautomerism
Q24529117Identification of critical elements in the tRNA acceptor stem and T(Psi)C loop necessary for human immunodeficiency virus type 1 infectivity
Q28646853Identification of tRNAs incorporated into wild-type and mutant human immunodeficiency virus type 1
Q36685453In vitro enzymatic activity of human immunodeficiency virus type 1 reverse transcriptase mutants in the highly conserved YMDD amino acid motif correlates with the infectious potential of the proviral genome
Q36637578Incorporation of excess wild-type and mutant tRNA(3Lys) into human immunodeficiency virus type 1.
Q35907165Inhibition of the p66/p51 form of human immunodeficiency virus reverse transcriptase by tRNALys
Q28359887Inhibitors of human immunodeficiency virus type 1 reverse transcriptase target distinct phases of early reverse transcription
Q37324137Initiation of (-) strand DNA synthesis from tRNA(3Lys) on lentiviral RNAs: implications of specific HIV-1 RNA-tRNA(3Lys) interactions inhibiting primer utilization by retroviral reverse transcriptases.
Q36944642Initiator methionine tRNA is essential for Ty1 transposition in yeast
Q33933045Interaction between retroviral U5 RNA and the T psi C loop of the tRNA(Trp) primer is required for efficient initiation of reverse transcription
Q38336483Interaction of HIV-1 reverse transcriptase with a synthetic form of its replication primer, tRNALys,3
Q34611173Interaction of retroviral nucleocapsid proteins with transfer RNAPhe: a lead ribozyme and 1H NMR study
Q34813558Intra-tRNA distance measurements for nucleocapsid proteindependent tRNA unwinding during priming of HIV reverse transcription
Q42860147Jean-Luc Darlix: Renaissance scientist and retrovirologist par excellence
Q34673247Mapping the RNA binding sites for human immunodeficiency virus type-1 gag and NC proteins within the complete HIV-1 and -2 untranslated leader regions.
Q36833119Mapping the human miRNA interactome by CLASH reveals frequent noncanonical binding
Q36629265Minimal sequence requirements of a functional human immunodeficiency virus type 1 primer binding site.
Q28757184Mn2+ suppressor mutations and biochemical communication between Ty1 reverse transcriptase and RNase H domains
Q38300846Multiple molecular determinants for retrotransposition in a primer tRNA
Q28367663Mutated K65R recombinant reverse transcriptase of human immunodeficiency virus type 1 shows diminished chain termination in the presence of 2',3'-dideoxycytidine 5'-triphosphate and other drugs
Q38314018Mutated primer binding sites interacting with different tRNAs allow efficient murine leukemia virus replication
Q37600218Mutation in the primer binding site of the type 1 human immunodeficiency virus genome affects virus production and infectivity
Q35854311Mutations of basic amino acids of NCp7 of human immunodeficiency virus type 1 affect RNA binding in vitro
Q40009476Nucleocapsid mutations turn HIV-1 into a DNA-containing virus.
Q37086413Nucleocapsid protein function in early infection processes
Q38351251On the importance of the primer activation signal for initiation of tRNA(lys3)-primed reverse transcription of the HIV-1 RNA genome
Q28379170Phosphorothioate oligonucleotides derived from human immunodeficiency virus type 1 (HIV-1) primer tRNALys3 are strong inhibitors of HIV-1 reverse transcriptase and arrest viral replication in infected cells
Q35820362Preferential incorporation of nucleoside analogs after template switching during human immunodeficiency virus reverse transcription
Q35875824Primer tRNA3Lys on the viral genome exists in unextended and two-base extended forms within mature human immunodeficiency virus type 1.
Q28775697Primer tRNAs for reverse transcription
Q33845445Proteolytic processing of the p2/nucleocapsid cleavage site is critical for human immunodeficiency virus type 1 RNA dimer maturation.
Q40115966Purification and characterization of recombinant equine infectious anemia virus reverse transcriptase
Q37130872RNA pseudoknots that inhibit human immunodeficiency virus type 1 reverse transcriptase
Q24675393Reduced replication of human immunodeficiency virus type 1 mutants that use reverse transcription primers other than the natural tRNA(3Lys)
Q39594951Replication and pathogenicity of primer binding site mutants of SL3-3 murine leukemia viruses
Q35848047Replication of avian leukosis viruses with mutations at the primer binding site: use of alternative tRNAs as primers
Q35313526Retrons and multicopy single-stranded DNA
Q38218411Retrospective on the all-in-one retroviral nucleocapsid protein
Q33753606Retroviral nucleocapsid protein-metal ion interactions: folding and sequence variants
Q35260021Reverse Transcriptase and Cellular Factors: Regulators of HIV-1 Reverse Transcription.
Q37248161Reverse transcriptase of human immunodeficiency virus can use either human tRNA(3Lys) or Escherichia coli tRNA(2Gln) as a primer in an in vitro primer-utilization assay
Q28646824Role of Pr160gag-pol in mediating the selective incorporation of tRNA(Lys) into human immunodeficiency virus type 1 particles
Q39578721Role of the N-terminal zinc finger of human immunodeficiency virus type 1 nucleocapsid protein in virus structure and replication
Q40405750Secondary structure of the HIV-2 leader RNA comprising the tRNA-primer binding site
Q30651852Selection of functional tRNA primers and primer binding site sequences from a retroviral combinatorial library: identification of new functional tRNA primers in murine leukemia virus replication
Q37437516Shaping and reshaping of salmonid genomes by amplification of tRNA-derived retroposons during evolution
Q37698214Site-specific crosslinking of 4-thiouridine-modified human tRNA(3Lys) to reverse transcriptase from human immunodeficiency virus type I.
Q27678299Solution Nuclear Magnetic Resonance Analyses of the Anticodon Arms of Proteinogenic and Nonproteinogenic tRNA Gly
Q40791350Specific binding of HIV-1 nucleocapsid protein to PSI RNA in vitro requires N-terminal zinc finger and flanking basic amino acid residues
Q35853488Specific initiation and switch to elongation of human immunodeficiency virus type 1 reverse transcription require the post-transcriptional modifications of primer tRNA3Lys
Q34364188Structural and functional properties of the HIV-1 RNA-tRNA(Lys)3 primer complex annealed by the nucleocapsid protein: comparison with the heat-annealed complex
Q35623899Structural basis of asymmetry in the human immunodeficiency virus type 1 reverse transcriptase heterodimer
Q41083490Subunit-selective mutagenesis indicates minimal polymerase activity in heterodimer-associated p51 HIV-1 reverse transcriptase
Q34769447The Interaction between tRNA(Lys) 3 and the primer activation signal deciphered by NMR spectroscopy
Q43183360The Mauriceville plasmid of Neurospora crassa: characterization of a novel reverse transcriptase that begins cDNA synthesis at the 3' end of template RNA.
Q38005738The accuracy of aminoacylation--ensuring the fidelity of the genetic code
Q35834904The central globular domain of the nucleocapsid protein of human immunodeficiency virus type 1 is critical for virion structure and infectivity
Q35067901The major RNA in prosomes of HeLa cells and duck erythroblasts is tRNA(Lys,3)
Q40416681The multimerization state of retroviral RNA is modulated by ammonium ions and affects HIV-1 full-length cDNA synthesis in vitro
Q33709362The nucleocapsid protein specifically anneals tRNALys-3 onto a noncomplementary primer binding site within the HIV-1 RNA genome in vitro
Q39615571The relationship between HIV-1 genome RNA dimerization, virion maturation and infectivity
Q39578385The role of nucleocapsid and U5 stem/A-rich loop sequences in tRNA(3Lys) genomic placement and initiation of reverse transcription in human immunodeficiency virus type 1.
Q41880988The tRNA recognition mechanism of folate/FAD-dependent tRNA methyltransferase (TrmFO)
Q33889345The yeast Ty3 retrotransposon contains a 5'-3' bipartite primer-binding site and encodes nucleocapsid protein NCp9 functionally homologous to HIV-1 NCp7
Q89204677Tie-Break: Host and Retrotransposons Play tRNA
Q34170023Time-resolved fluorescence investigation of the human immunodeficiency virus type 1 nucleocapsid protein: influence of the binding of nucleic acids
Q40790993Transactivation of the minus-strand DNA transfer by nucleocapsid protein during reverse transcription of the retroviral genome.
Q67678644Transfer RNA-like structure of the human Alu family: Implications of its generation mechanism and possible functions
Q39602865Transfer of primer binding site-mutated simian immunodeficiency virus vectors by genetically engineered artificial and hybrid tRNA-like primers.
Q40405385Two short basic sequences surrounding the zinc finger of nucleocapsid protein NCp10 of Moloney murine leukemia virus are critical for RNA annealing activity
Q34625646Two step synthesis of (-) strong-stop DNA by avian and murine reverse transcriptases in vitro.
Q35655392Use of gel retardation to analyze protein-nucleic acid interactions
Q34363183Use of terbium as a probe of tRNA tertiary structure and folding
Q37538534Variation of human immunodeficiency virus type-1 reverse transcriptase within the simian immunodeficiency virus genome of RT-SHIV.
Q36701114Viral DNA carried by human immunodeficiency virus type 1 virions
Q37111477Viral RNA annealing activities of human immunodeficiency virus type 1 nucleocapsid protein require only peptide domains outside the zinc fingers
Q40504840Viral RNA annealing activities of the nucleocapsid protein of Moloney murine leukemia virus are zinc independent
Q37130269When is it time for reverse transcription to start and go?
Q34891578Yeast Ty1 retrotransposon: the minus-strand primer binding site and a cis-acting domain of the Ty1 RNA are both important for packaging of primer tRNA inside virus-like particles
Q38874192tRNA wobble modifications and protein homeostasis