A High-Throughput Standard PCR-Based Genotyping Method for Determining Transgene Zygosity in Segregating Plant Populations

scientific article published on 24 July 2017

A High-Throughput Standard PCR-Based Genotyping Method for Determining Transgene Zygosity in Segregating Plant Populations is …
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scholarly articleQ13442814

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P356DOI10.3389/FPLS.2017.01252
P932PMC publication ID5522864
P698PubMed publication ID28791034

P2093author name stringJohnie N Jenkins
Dewayne D Deng
Jack C McCarty
Martin J Wubben
Ibrokhim Abdurakhmonov
Lige Geng
P2860cites workHigh-throughput droplet digital PCR system for absolute quantitation of DNA copy numberQ28251729
Real time quantitative PCRQ29616373
An evaluation of new and established methods to determine T-DNA copy number and homozygosity in transgenic plants.Q37252572
Next-generation sequencing is a robust strategy for the high-throughput detection of zygosity in transgenic maize.Q41483747
The Brassica napus receptor-like protein RLM2 is encoded by a second allele of the LepR3/Rlm2 blackleg resistance locusQ41490982
Phytochrome RNAi enhances major fibre quality and agronomic traits of the cotton Gossypium hirsutum L.Q47840643
Molecular cloning, differential expression, and functional characterization of a family of class I ubiquitin-conjugating enzyme (E2) genes in cotton (Gossypium).Q48259112
Fast-tracking determination of homozygous transgenic lines and transgene stacking using a reliable quantitative real-time PCR assayQ48701256
Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR.Q54164416
Estimating the copy number of transgenes in transformed rice by real-time quantitative PCR.Q54699191
Quantitative real-time PCR assay to detect transgene copy number in cotton (Gossypium hirsutum)Q80196304
P921main subjectplant populationQ122208088
P304page(s)1252
P577publication date2017-07-24
P1433published inFrontiers in Plant ScienceQ27723840
P1476titleA High-Throughput Standard PCR-Based Genotyping Method for Determining Transgene Zygosity in Segregating Plant Populations
P478volume8

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