Inducing single-cell suspension of BTI-TN5B1-4 insect cells: I. The use of sulfated polyanions to prevent cell aggregation and enhance recombinant protein production

scientific article published on May 1997

Inducing single-cell suspension of BTI-TN5B1-4 insect cells: I. The use of sulfated polyanions to prevent cell aggregation and enhance recombinant protein production is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1002/(SICI)1097-0290(19970505)54:3<191::AID-BIT1>3.0.CO;2-A
P698PubMed publication ID18634086

P2093author name stringShuler ML
Wood HA
Dee KU
P2860cites workScreening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression systemQ34247472
Optimization of growth methods and recombinant protein production in BTI-Tn-5B1-4 insect cells using the baculovirus expression systemQ34311763
Active human erythropoietin expressed in insect cells using a baculovirus vector: a role for N-linked oligosaccharideQ36420538
Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vectorQ37539177
Colloid titration of heparin using Cat-Floc (polydiallyldimethyl ammonium chloride) as standard polycationQ39211358
Production of human alkaline phosphatase, a secreted, glycosylated protein, from a baculovirus expression system and the attachment-dependent cell line Trichoplusia ni BTI-Tn 5B1-4 using a split-flow, air-lift bioreactorQ41518924
Expression of three recombinant proteins using baculovirus vectors in 23 insect cell linesQ41709678
Heparin-induced aggregation of lymphoid cellsQ42812627
Inhibition of Autographa californica nuclear polyhedrosis virus replication in high-density Trichoplusia ni cell culturesQ45393191
Human tissue-type plasminogen activator synthesized by using a baculovirus vector in insect cells compared with human plasminogen activator produced in mouse cells.Q52459993
Changes in animal cell natural aggregates in suspended batch culturesQ58754783
Interaction of dextran sulfate with phospholipid surfaces and liposome aggregation and fusionQ68681080
Induction of fusion in aggregated and nonaggregated liposomes bearing cationic detergentsQ69675273
Determination of the Charge Content at the Surface of Cells Using a Colloid Titration TechniqueQ72106955
P433issue3
P304page(s)191-205
P577publication date1997-05-01
P1433published inBiotechnology and BioengineeringQ4915339
P1476titleInducing single-cell suspension of BTI-TN5B1-4 insect cells: I. The use of sulfated polyanions to prevent cell aggregation and enhance recombinant protein production
P478volume54

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cites work (P2860)
Q37573768A novel baculovirus vector for the production of nonfucosylated recombinant glycoproteins in insect cells
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Q51722429Arginine as an eluent overcomes the hindrance of monoclonal antibody quantification by dextran sulfate in protein A affinity chromatography.
Q88428346Chemically controlled aggregation of pluripotent stem cells
Q35217908Combination of dextran sulfate and recombinant trypsin on aggregation of Chinese hamster ovary cells
Q40768435Design of an efficient medium for insect cell growth and recombinant protein production.
Q39918105Detachment factors for enhanced carrier to carrier transfer of CHO cell lines on macroporous microcarriers
Q42326959Development and improvement of a serum-free suspension process for the production of recombinant adenoviral vectors using HEK293 cells
Q39918171Effect of Partial Medium Replacement on Cell Growth and Protein Production for the High-Fivetrade mark insect cell line
Q39272620Gene expression profiling for mechanistic understanding of cellular aggregation in mammalian cell perfusion cultures
Q40963386Glycosylation of a recombinant protein in the Tn5B1-4 insect cell line: influence of ammonia, time of harvest, temperature, and dissolved oxygen
Q39918484Growth, metabolism and baculovirus production in suspension cultures of an Anticarsia gemmatalis cell line
Q39686931Heparin promotes suspension adaptation process of CHO-TS28 cells by eliminating cell aggregation
Q40963398High-level expression of secreted glycoproteins in transformed lepidopteran insect cells using a novel expression vector
Q41110816Inducing single-cell suspension of BTI-TN5B1-4 insect cells: II. The effect of sulfated polyanions on baculovirus infection
Q36685587Large-scale transfection of mammalian cells for the fast production of recombinant protein
Q39581617NMR-based metabolomics of mammalian cell and tissue cultures
Q45736726Parameters that determine virus adsorption kinetics: toward the design of better infection strategies for the insect cell - baculovirus expression system.
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Q38739041The molecular weight and concentration of dextran sulfate affect cell growth and antibody production in CHO cell cultures

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