Movement of the 3'-end of tRNA through the peptidyl transferase centre and its inhibition by antibiotics

scientific article published on April 1997

Movement of the 3'-end of tRNA through the peptidyl transferase centre and its inhibition by antibiotics is …
instance of (P31):
review articleQ7318358
scholarly articleQ13442814

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P356DOI10.1016/S0014-5793(97)00261-5
P698PubMed publication ID9136892

P50authorBo Torben PorseQ38318663
P2093author name stringGarrett RA
Woolley P
Vester B
Kirillov S
P2860cites workCrystal structure of the ternary complex of Phe-tRNAPhe, EF-Tu, and a GTP analogQ27729310
The crystal structure of elongation factor G complexed with GDP, at 2.7 A resolutionQ27730883
Cooperative and Antagonistic Interactions of Peptidyl-tRNA and Antibiotics with Bacterial RibosomesQ28324154
Topography of the E site on the Escherichia coli ribosomeQ34043424
Inhibition of ribosomal peptidyltransferase by chloramphenicol. Kinetic studiesQ34183335
Labeling the peptidyltransferase center of the Escherichia coli ribosome with photoreactive tRNA(Phe) derivatives containing azidoadenosine at the 3' end of the acceptor arm: a model of the tRNA-ribosome complexQ34289140
Unusual resistance of peptidyl transferase to protein extraction proceduresQ34436822
The molecular basis of the inhibitory activities of type A and type B synergimycins and related antibiotics on ribosomesQ34664093
A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosomeQ34719982
Aminoacyl-tRNA binding at the recognition site is the first step of the elongation cycle of protein synthesisQ35026380
Stem-loop IV of 5S rRNA lies close to the peptidyltransferase centerQ35216952
Chemical probing of the tRNA--ribosome complexQ35346450
Nascent polypeptide chains exit the ribosome in the same relative position in both eucaryotes and procaryotesQ36208211
Cloning of a lincosamide resistance determinant from Streptomyces caelestis, the producer of celesticetin, and characterization of the resistance mechanismQ36256443
Nascent polypeptide chains emerge from the exit domain of the large ribosomal subunit: immune mapping of the nascent chainQ36295457
Location of exit channel for nascent protein in 80S ribosomeQ70020849
On substrate specificity of the donor site of the Escherichia coli ribosomal peptidyl transferase center. Synthesis of dipeptides from 3'-terminal fragments of aminoacyl-tRNAQ70253405
Kinetic aspects of tetracycline action on the acceptor (A) site of Escherichia coli ribosomesQ70253420
Quantitative study of interaction of deacylated tRNA with Escherichia coli ribosomes. Role of 50 S subunits in formation of the E siteQ70262602
Binding of yeast tRNAphe anticodon arm to Escherichia coli 30 S ribosomesQ70264337
Cooperative effects in the peptidyltransferase center of Escherichia coli ribosomesQ70281466
Elongating ribosomes in vivo are refractory to erythromycinQ70372908
The sequence of reactions leading to the synthesis of a peptide bond on reticulocyte ribosomesQ71005588
Catalysis of peptide bond formation by 50 s ribosomal subunits from Escherichia coliQ71213755
Mutations in the peptidyl transferase center of 23 S rRNA reveal the site of action of sparsomycin, a universal inhibitor of translationQ71396621
Transesterification by Peptidyl TransferaseQ71405072
Sparsophenicol: a new synthetic hybrid antibiotic inhibiting ribosomal peptide synthesisQ71455275
Non-exclusion principle of Ac-Phe-tRNAPhe interaction with the donor and acceptor sites of Escherichia coli ribosomesQ71469894
Mechanism of codon-anticodon interaction in ribosomes: codon-anticodon interaction of aminoacyl-tRNA at the ribosomal donor siteQ71889057
Fine structure of the peptidyl transferase centre on 23 S-like rRNAs deduced from chemical probing of antibiotic-ribosome complexesQ72134539
Ribosome-catalysed peptidyl transfer: effects of some inhibitors of protein synthesisQ72291068
tRNA binding sites of ribosomes from Escherichia coliQ72382430
70-S ribosomes of Escherichia coli have an additional site for deacylated tRNA bindingQ72683868
Synergism between the GTPase activities of EF-Tu.GTP and EF-G.GTP on empty ribosomes. Elongation factors as stimulators of the ribosomal oscillation between two conformationsQ72732431
THE BINDING OF S-RNA BY ESCHERICHIA COLI RIBOSOMESQ76571928
BINDING OF TRANSFER RIBONUCLEIC ACID TO RIBOSOMES ENGAGED IN PROTEIN SYNTHESIS: NUMBER AND PROPERTIES OF RIBOSOMAL BINDING SITESQ77208552
Inactivation and reactivation of the acceptor binding site of ribosomal peptidyl transferaseQ77916680
(14C)LYSINE PEPTIDES SYNTHESIZED IN AN IN VITRO ESCHERICHIA COLI SYSTEM IN THE PRESENCE OF CHLORAMPHENICOLQ78546402
Binding of puromycin to E. coli ribosomes. Effects of puromycin analogues and peptide bond formation inhibitorsQ86657638
Kinetic studies of peptide bond formation. Effect of chloramphenicolQ86657732
2′(3′)-O-N -formylmethionyl)-adenosine-5′-phosphate, a new donor substrate in peptidyl transferase catalyzed reactionsQ93752725
Peptidyl-transferase activity of Escherichia coli ribosomes digested by ribonuclease T 1Q95347638
Three tRNA binding sites on Escherichia coli ribosomesQ36366220
Studies on the formation of transfer ribonucleic acid-ribosome complexes. XI. Antibiotic effects on phenylalanyl-oligonucleotide binding to ribosomesQ36445718
Stepwise synthesis of a tripeptideQ36476172
Chemical, biochemical and genetic endeavours characterizing the interaction of sparsomycin with the ribosomeQ36700664
Sites of interaction of the CCA end of peptidyl-tRNA with 23S rRNA.Q37494738
Effect of nucleotide substitution on the peptidyltransferase activity of 2'(3')-O-(aminoacyl) oligonucleotidesQ38342268
The importance of highly conserved nucleotides in the binding region of chloramphenicol at the peptidyl transfer centre of Escherichia coli 23S ribosomal RNA.Q38346185
Destabilization of codon-anticodon interaction in the ribosomal exit siteQ38349033
Effect of codon shortening and the antibiotics viomycin and sparsomycin upon the behaviour of bound aminoacyl-tRNA. Decoding at the ribosomal A siteQ38354682
Constraints on the accuracy of messenger RNA movementQ39844036
Lincosamide antibiotics stimulate dissociation of peptidyl-tRNA from ribosomesQ39866740
Functional interactions within 23S rRNA involving the peptidyltransferase center.Q39936039
Chloramphenicol-erythromycin resistance mutations in a 23S rRNA gene of Escherichia coliQ39980011
Role for the highly conserved region of domain IV of 23S-like rRNA in subunit-subunit interactions at the peptidyl transferase centreQ40393879
Mapping the path of the nascent peptide chain through the 23S in the 50S ribosomal subunitQ40394669
Site-directed cross-linking studies on theE.colitRNA ribosome complex: determination of sites labelled with an aromatic azide attached to the variable loop or aminoacyl group of tRNAQ40405431
Pseudouridine and O2-methylated nucleosides. Significance of their selective occurrence in rRNA domains that function in ribosome-catalyzed synthesis of the peptide bonds in proteinsQ40437398
Mechanism of codon-anticodon interaction in ribosomes. Direct functional evidence that isolated 30S subunits contain two codon-specific binding sites for transfer RNA.Q40498628
Comparative study of the interaction of polyuridylic acid with 30S subunits and 70S ribosomes of Escherichia coliQ40498803
Interaction of the antibiotics clindamycin and lincomycin with Escherichia coli 23S ribosomal RNA.Q40534214
Interaction of N-acetyl-phenylalanyl-tRNAPhewith 70S ribosomes of Escherichia coliQ40576447
The 530 loop of 16S rRNA: a signal to EF-Tu?Q40735046
Antibiotics as probes of ribosome structure: binding of chloramphenicol and erythromycin to polyribosomes; effect of other antibioticsQ40943039
Genetic probes of ribosomal RNA functionQ41043649
Antibiotic inhibition of the movement of tRNA substrates through a peptidyl transferase cavityQ41043665
Peptidyl transferase and beyondQ41043759
Photo-affinity labelling at the peptidyl transferase centre reveals two different positions for the A- and P-sites in domain V of 23S rRNAQ41095178
Sequence at the Site of Attachment of an Affinity-Label Derivative of Puromycin on 23-S Ribosomal RNA of Escherichia coli RibosomesQ41354069
A model of protein synthesis based on cryo-electron microscopy of the E. coli ribosomeQ41634310
Mechanism of action of sparsomycin in protein synthesisQ41888184
Does the channel for nascent peptide exist inside the ribosome? Immune electron microscopy studyQ43431144
Interaction of tRNA with 23S rRNA in the ribosomal A, P, and E sitesQ43666149
An apparent conformational change in tRNA(Phe) that is associated with the peptidyl transferase reactionQ43756721
Binding of aminoacyl-tRNA to ribosomes promoted by elongation factor Tu. Studies on the role of GTP hydrolysisQ43927656
Intermediate states in the movement of transfer RNA in the ribosomeQ44026748
Movement of tRNA but not the nascent peptide during peptide bond formation on ribosomesQ44394996
The synthesis of polyphenylalanine on ribosomes to which erythromycin is boundQ44512579
Chloramphenicol, erythromycin, carbomycin and vernamycin B protect overlapping sites in the peptidyl transferase region of 23S ribosomal RNA.Q46131638
Peptide bond formation on the ribosome. Structural requirements for inhibition of protein synthesis and of release of peptides from peptidyl-tRNA on bacterial and mammalian ribosomes by aminoacyl and nucleotidyl analogues of puromycinQ47748838
Structural basis for inhibition of protein synthesis by the aminoacyl-aminohexosyl-cytosine group of antibioticsQ47836836
Erythromycin binding is reduced in ribosomes with conformational alterations in the 23 S rRNA peptidyl transferase loopQ50125052
Affinities of tRNA binding sites of ribosomes from Escherichia coli.Q52639241
Decoding at the ribosomal A site: antibiotics, misreading and energy of aminoacyl-tRNA binding.Q53045644
Conserved nucleotides of 23 S rRNA located at the ribosomal peptidyltransferase center.Q54575115
The donor substrate site within the peptidyl transferase loop of 23 S rRNA and its putative interactions with the CCA-end of N-blocked aminoacyl-tRNA(Phe).Q54575472
The involvement of two distinct regions of 23 S ribosomal RNA in tRNA selection.Q54598388
Evidence for RNA in the peptidyl transferase center of Escherichia coli ribosomes as indicated by fluorescenceQ54667323
Peptidyl puromycin synthesis; effect of several antibiotics which act on 50 S ribosomal subunits.Q54672051
Identification of intermolecular RNA cross-links at the subunit interface of the Escherichia coli ribosomeQ54681970
Fluorescence characterization of the environment encountered by nascent polyalanine and polyserine as they exit Escherichia coli ribosomes during translation.Q54682226
Kinetics of inhibition of peptide bond formation on bacterial ribosomesQ54684990
Binding sites of the antibiotics pactamycin and celesticetin on ribosomal RNAsQ54695180
Partial release of AcPhe-Phe-tRNA from ribosomes during poly(U)-dependent poly(Phe) synthesis and the effects of chloramphenicol.Q54706144
The extension of polyphenylalanine and polylysine peptides on Escherichia coli ribosomes.Q54709815
Photochemical cross-linking of yeast tRNA(Phe) containing 8-azidoadenosine at positions 73 and 76 to the Escherichia coli ribosome.Q54740158
Mechanism of ribosomal translocation. tRNA binds transiently to an exit site before leaving the ribosome during translocation.Q54762563
Structural characteristics and classification of some tRNA-binding sites of elongating Escherichia coli ribosome.Q54776820
Biochemical and kinetic characteristics of the interaction of the antitumor antibiotic sparsomycin with prokaryotic and eukaryotic ribosomesQ57977649
Comparison of Escherichia coli tRNAPhe in the Free State, in the Ternary Complex and in the Ribosomal A and P Sites by Chemical ProbingQ58032651
Action of Sparsomycin on Ribosome-catalysed Peptidyl TransferQ59012849
Translocation in Protein Synthesis: A Hybrid Structure ModelQ59035609
A Sparsomycin-resistant Mutant ofHalobacterium salinariumLacks a Modification at Nucleotide U2603 in the Peptidyl Transferase Centre of 23 S rRNAQ61862594
Mapping Important Nucleotides in the Peptidyl Transferase Centre of 23 S rRNA using a Random Mutagenesis ApproachQ61862598
Analogues of chloramphenicol: circular dichroism spectra, inhibition of ribosomal peptidyltransferase, and possible mechanism of actionQ67258528
Quantitative study of the interaction of aminoacyl-tRNA with the a site of Escherichia coli ribosomes: equilibrium and kinetic parameters of binding in the absence of EF-Tu factor and GTPQ67300947
Mechanism of codon-anticodon interaction in ribosomes: comparative study of interaction of Phe-tRNAPhe and N-acetyl-Phe-tRNAPhe with the donor site of Escherichia coli ribosomesQ67300955
Puromycin reaction of the A-site bound peptidyl-tRNAQ67492883
Puromycin reaction for the A site-bound peptidyl-tRNAQ67737608
Extension of Watson's model for the elongation cycle of protein biosynthesisQ68113356
Bacterial-protein synthesis. A novel system for studying antibiotic action in vivoQ68522445
Kinetic studies on ribosomal peptidyltransferase. The behaviour of the inhibitor blasticidin SQ68858630
Localization of elongation factor Tu on the ribosomeQ68861050
A tunnel in the large ribosomal subunit revealed by three-dimensional image reconstructionQ69004129
Affinity labelling of ribosomal peptidyl transferase by a puromycin analogueQ69339534
The effect of GTP hydrolysis and transpeptidation on the arrangement of aminoacyl-tRNA at the A-site of Escherichia coli 70 S ribosomesQ69381083
Ab initio calculations on large molecules using molecular fragments. Structural correlations between natural substrate moieties and some antibiotic inhibitors of peptidyl transferaseQ69767284
A Structural Requirement for Codon-Anticodon Interaction on the RibosomeQ69904549
A Model of the Functioning Ribosome: Locking and Unlocking of the Ribosome SubparticlesQ69904555
Binding of blasticidin S to ribosomesQ69916596
Substrate Specificity of Escherichia coli Peptidyl-TransferaseQ69928639
Effects of macrolides on peptide-bond formation and translocationQ69941248
P433issue3
P407language of work or nameEnglishQ1860
P304page(s)223-233
P577publication date1997-04-01
P1433published inFEBS LettersQ1388051
P1476titleMovement of the 3'-end of tRNA through the peptidyl transferase centre and its inhibition by antibiotics
P478volume406