Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization

scientific article published on September 2017

Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1371/JOURNAL.PONE.0184162
P932PMC publication ID5581180
P698PubMed publication ID28863186

P50authorMarcin OlszewskiQ59679557
Beata KrawczykQ59693318
Maciej BilekQ86825038
P2093author name stringMarta Śpibida
P2860cites workDNA polymerase hybrids derived from the family-B enzymes of Pyrococcus furiosus and Thermococcus kodakarensis: improving performance in the polymerase chain reactionQ21131094
Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticusQ24642284
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymeraseQ26778389
Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meatQ28344989
Glutathione S-transferases. The first enzymatic step in mercapturic acid formationQ29547415
Homozygous defect in HIV-1 coreceptor accounts for resistance of some multiply-exposed individuals to HIV-1 infectionQ29614956
Prokaryotic DNA polymerase I: evolution, structure, and "base flipping" mechanism for nucleotide selectionQ30667746
Related bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificitiesQ31054232
Purification and characterization of a common soil component which inhibits the polymerase chain reactionQ33180848
Capacity of nine thermostable DNA polymerases To mediate DNA amplification in the presence of PCR-inhibiting samplesQ33717098
Real-time monitoring of DNA polymerase activity using molecular beacon.Q51223188
Application of SSB-like protein from Thermus aquaticus in multiplex PCR of human Y-STR markers identification.Q54673256
Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli.Q54705637
Characterization and PCR application of a thermostable DNA polymerase from Thermococcus pacificusQ57960325
[Isolation and properties of DNA polymerase from extreme thermophylic bacteria Thermus aquaticus YT-1]Q71206998
Inactivation of the 5'-3' exonuclease of Thermus aquaticus DNA polymeraseQ71578006
Large contributions of coupled protonation equilibria to the observed enthalpy and heat capacity changes for ssDNA binding to Escherichia coli SSB proteinQ73025524
Rapid assay for detection of methicillin-resistant Staphylococcus aureus using multiplex PCRQ73049328
Fluorescence-based DNA polymerase assayQ73402769
Effects of heparin on polymerase chain reaction for blood white cells.Q33861924
Purification and characterization of PCR-inhibitory components in blood cellsQ33971131
Amplification efficiency of thermostable DNA polymerasesQ33972753
Characterization of a Single-Stranded DNA-Binding-Like Protein from Nanoarchaeum equitans--A Nucleic Acid Binding Protein with Broad Substrate SpecificityQ34044008
Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving.Q34091757
High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity.Q34350767
Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria Desulfotalea psychrophila, Flavobacterium psychrophilum, Psychrobacter arcticus, Psychrobacter cryohalolentis, Psychromonas ingrahamii, Psychroflexus torquis, aQ35146048
Structure and mechanism of DNA polymerasesQ36288580
Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samplesQ37128112
Two highly thermostable paralogous single-stranded DNA-binding proteins from Thermoanaerobacter tengcongensis.Q38292017
Cloning, overexpression, and purification of the recombinant His-tagged SSB protein of Escherichia coli and use in polymerase chain reaction amplificationQ38324458
Identification and characterization of single-stranded-DNA-binding proteins from Thermus thermophilus and Thermus aquaticus - new arrangement of binding domainsQ38362223
Modified DNA polymerases for PCR troubleshootingQ38994900
Thermodynamics of the binding of Thermus aquaticus DNA polymerase to primed-template DNAQ39936185
Optimization of the PCR program for RAPD analysisQ40422504
A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitroQ40695789
A quantitative fluorescence-based steady-state assay of DNA polymeraseQ41934507
Cloning, expression, and characterization of thermostable DNA polymerase from Thermoanaerobacter yonseiensisQ42683639
DNA-Binding Domain of DNA Ligase from the Thermophilic Archaeon Pyrococcus abyssi: Improving Long-Range PCR and Neutralization of Heparin's Inhibitory EffectQ43024757
Recombinant His-tagged DNA polymerase. II. Cloning and purification of Thermus aquaticus recombinant DNA polymerase (Stoffel fragment).Q43026169
Enhancing the processivity of a family B-type DNA polymerase of Thermococcus onnurineus and application to long PCR.Q43723654
Structure and enzymatic properties of a chimeric bacteriophage RB69 DNA polymerase and single-stranded DNA binding protein with increased processivityQ43967675
Methods of analyzing processivityQ45283670
Cloning and expression in Escherichia coli of the recombinant his-tagged DNA polymerases from Pyrococcus furiosus and Pyrococcus woeseiQ48018781
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue9
P407language of work or nameEnglishQ1860
P921main subjectNanoarchaeum equitansQ221936
P304page(s)e0184162
P577publication date2017-09-01
P1433published inPLOS OneQ564954
P1476titleFusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization
P478volume12

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cites work (P2860)
Q99418140Engineered viral DNA polymerase with enhanced DNA amplification capacity: a proof-of-concept of isothermal amplification of damaged DNA
Q89688947PCR inhibition in qPCR, dPCR and MPS-mechanisms and solutions

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