Effects of recombinant protein expression on green fluorescent protein diffusion in Escherichia coli

scientific article published on June 2009

Effects of recombinant protein expression on green fluorescent protein diffusion in Escherichia coli is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1021/BI9004107
P932PMC publication ID4529995
P698PubMed publication ID19413350

P2093author name stringGary J Pielak
Michael Chua
Nancy L Thompson
Kristin M Slade
Rachael Baker
P2860cites workProtein measurement with the Folin phenol reagentQ20900776
Mobility measurement by analysis of fluorescence photobleaching recovery kineticsQ24536888
The molecular structure of green fluorescent proteinQ27758957
The influence of macromolecular crowding and macromolecular confinement on biochemical reactions in physiological mediaQ28209077
Calmodulin: an introductionQ28265607
Maltose/maltodextrin system of Escherichia coli: transport, metabolism, and regulationQ28266679
The effect of volume occupancy upon the thermodynamic activity of proteins: some biochemical consequencesQ30413607
Studying protein dynamics in living cellsQ30674118
Quantitative protein stability measurement in vivoQ31011932
Export of active green fluorescent protein to the periplasm by the twin-arginine translocase (Tat) pathway in Escherichia coli.Q31727465
Protein mobility and diffusive barriers in Escherichia coli: consequences of osmotic stressQ33282905
Tracer diffusion of globular proteins in concentrated protein solutionsQ33569770
Cytoarchitecture and physical properties of cytoplasm: volume, viscosity, diffusion, intracellular surface areaQ33769782
Cytoplasmic viscosity near the cell plasma membrane: translational diffusion of a small fluorescent solute measured by total internal reflection-fluorescence photobleaching recoveryQ34040455
Macromolecular crowding: an important but neglected aspect of the intracellular environmentQ34141548
FlgM gains structure in living cellsQ34189211
Macromolecular crowding and its role as intracellular signalling of cell volume regulationQ34312748
Production and ultrastructure of lysozyme and ethylenediaminetetraacetate-lysozyme spheroplasts of Escherichia coliQ35147128
Translocation of alpha-synuclein expressed in Escherichia coliQ35759527
Influence of macromolecular crowding upon the stability and state of association of proteins: predictions and observationsQ36178505
Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations.Q36303609
Methods of changing biopolymer volume fraction and cytoplasmic solute concentrations for in vivo biophysical studies.Q36944206
Protein nuclear magnetic resonance under physiological conditionsQ37104114
Crowding effects on diffusion in solutions and cellsQ37197981
Alpha-synuclein misfolding and neurodegenerative diseasesQ37294354
Tau oligomerization: a role for tau aggregation intermediates linked to neurodegenerationQ37347086
Characterization of DsbC, a periplasmic protein of Erwinia chrysanthemi and Escherichia coli with disulfide isomerase activityQ37631345
Protein mobility in the cytoplasm of Escherichia coli.Q38330173
Metabolic buffering exerted by macromolecular crowding on DNA-DNA interactions: origin and physiological significanceQ38357394
Crowding and confinement effects on protein diffusion in vivoQ38501075
Differential dynamical effects of macromolecular crowding on an intrinsically disordered protein and a globular protein: implications for in-cell NMR spectroscopyQ39411140
Leaderless polypeptides efficiently extracted from whole cells by osmotic shockQ39846925
Reversible photobleaching of enhanced green fluorescent proteins.Q40428328
Macromolecular crowding: biochemical, biophysical, and physiological consequencesQ40488946
Periplasmic space in Salmonella typhimurium and Escherichia coliQ40820160
Diffusion of green fluorescent protein in three cell environments in Escherichia coliQ41673126
Quantifying green fluorescent protein diffusion in Escherichia coli by using continuous photobleaching with evanescent illuminationQ42569734
A fluorescence resonance energy transfer sensor based on maltose binding proteinQ44585946
A high-throughput capillary assay for bacterial chemotaxisQ44590421
Roles of cytoplasmic osmolytes, water, and crowding in the response of Escherichia coli to osmotic stress: biophysical basis of osmoprotection by glycine betaineQ44633080
Binding of Ca2+ and Zn2+ to human nuclear S100A2 and mutant proteins.Q47828552
A new method for purification of recombinant human alpha-synuclein in Escherichia coli.Q54484969
Macromolecular crowding increases structural content of folded proteinsQ57821854
Molecular characterisation of recombinant green fluorescent protein by fluorescence correlation microscopy.Q57902867
In-Cell NMR SpectroscopyQ57903789
P433issue23
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
P304page(s)5083-5089
P577publication date2009-06-01
P1433published inBiochemistryQ764876
P1476titleEffects of recombinant protein expression on green fluorescent protein diffusion in Escherichia coli
P478volume48

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cites work (P2860)
Q33637165A bioreactor for in-cell protein NMR.
Q51547363A microfluidic concentrator array for quantitative predation assays of predatory microbes.
Q50688189Cooperation and cheating in microbial exoenzyme production--theoretical analysis for biotechnological applications.
Q53263610Diffusion-limited encounter rate in a three-dimensional lattice of connected compartments studied by Brownian-dynamics simulations.
Q36419597Disordered Protein Diffusion under Crowded Conditions.
Q34043454Evaluation of pulsed-FRAP and conventional-FRAP for determination of protein mobility in prokaryotic cells
Q28535612In-cell NMR characterization of the secondary structure populations of a disordered conformation of α-synuclein within E. coli cells
Q84947879In-cell NMR spectroscopy
Q41652149In-cell protein NMR and protein leakage
Q33626348Protein (19)F NMR in Escherichia coli
Q41865675Size dependence of protein diffusion in the cytoplasm of Escherichia coli
Q33683505The STINT-NMR method for studying in-cell protein-protein interactions.

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