Broad-host-range expression vectors with tightly regulated promoters and their use to examine the influence of TraR and TraM expression on Ti plasmid quorum sensing

scientific article published on 7 July 2008

Broad-host-range expression vectors with tightly regulated promoters and their use to examine the influence of TraR and TraM expression on Ti plasmid quorum sensing is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1128/AEM.01098-08
P932PMC publication ID2519271
P698PubMed publication ID18606801
P5875ResearchGate publication ID5245807

P2093author name stringStephen K Farrand
R Martin Roop
Sharik R Khan
Jennifer Gaines
P2860cites workThe tac promoter: a functional hybrid derived from the trp and lac promotersQ24602390
Structural basis for antiactivation in bacterial quorum sensingQ27648725
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoterQ27860697
Improved conditions for production of recombinant plant sesquiterpene synthases in Escherichia coli.Q54457738
R Factor Transfer in Rhizobium leguminosarumQ54634514
The use of lac-type promoters in control analysis.Q54663273
Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectorsQ70196959
Modulating quorum sensing by antiactivation: TraM interacts with TraR to inhibit activation of Ti plasmid conjugal transfer genesQ73189438
The antiactivator TraM interferes with the autoinducer-dependent binding of TraR to DNA by interacting with the C-terminal region of the quorum-sensing activatorQ73538356
Molecular basis of transcriptional antiactivation. TraM disrupts the TraR-DNA complex through stepwise interactionsQ80256815
Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoterQ27861016
beta-Glucuronidase from Escherichia coli as a gene-fusion markerQ28300102
Regulation of D-xylose metabolism in Caulobacter crescentus by a LacI-type repressorQ29346652
A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genesQ29547324
Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettesQ29615258
Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vectorQ29615289
Construction of a derivative of Agrobacterium tumefaciens C58 that does not mutate to tetracycline resistanceQ31980272
lac repressor forms loops with linear DNA carrying two suitably spaced lac operatorsQ33930312
The three operators of the lac operon cooperate in repressionQ34028027
Long-term and homogeneous regulation of the Escherichia coli araBAD promoter by use of a lactose transporter of relaxed specificityQ34063831
A broad-host-range expression vector series including a Ptac test plasmid and its application in the expression of the dod gene of Serratia marcescens (coding for ribulose-5-phosphate 3-epimerase) in Pseudomonas stutzeriQ34066604
17 Genetics of Caulobacter crescentusQ34508818
Characterization and genetic complementation of a Brucella abortus high-temperature-requirement A (htrA) deletion mutant.Q34539325
Agrobacterium tumefaciens DNA and PS8 Bacteriophage DNA Not Detected in Crown Gall TumorsQ35113723
Activity of the Agrobacterium Ti plasmid conjugal transfer regulator TraR is inhibited by the product of the traM geneQ35580586
Signal-dependent DNA binding and functional domains of the quorum-sensing activator TraR as identified by repressor activity.Q35592391
Isolation and characterization of a xylose-dependent promoter from Caulobacter crescentusQ35619172
A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumor metaboliteQ36107445
Controlled expression of the transcriptional activator gene virG in Agrobacterium tumefaciens by using the Escherichia coli lac promoterQ36129713
Vector for regulated expression of cloned genes in a wide range of gram-negative bacteriaQ36249220
Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations.Q36303609
18 Genetic Analysis of AgrobacteriumQ36439585
Plasmid required for virulence of Agrobacterium tumefaciensQ36605188
Cell-cell communication in the plant pathogen Agrobacterium tumefaciensQ36732525
Induction and loss of Ti plasmid conjugative competence in response to the acyl-homoserine lactone quorum-sensing signalQ36747386
Quorum sensing but not autoinduction of Ti plasmid conjugal transfer requires control by the opine regulon and the antiactivator TraM.Q39499089
The TOL plasmid pWW0 xylN gene product from Pseudomonas putida is involved in m-xylene uptake.Q39505238
Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control: I. DNA fragment insertion at the lacZ EcoRI restriction siteQ39656533
Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control. II. DNA fragment insertion at the vicinity of the lac UV5 promoterQ39656537
A new regulatory element modulates homoserine lactone-mediated autoinduction of Ti plasmid conjugal transferQ39835322
Construction and comparison of Escherichia coli whole-cell biosensors capable of detecting aromatic compoundsQ42471629
Isolation and molecular characterization of a novel broad-host-range plasmid from Bordetella bronchiseptica with sequence similarities to plasmids from gram-positive organisms.Q42599637
THE DEVELOPMENT OF THE BACTERIAL FLORA IN THE GASTROINTESTINAL TRACT OF MICE.Q42830078
Inhibition of the Agrobacterium tumefaciens TraR quorum-sensing regulator. Interactions with the TraM anti-activator.Q43785524
Electroporation of a suicide plasmid bearing a transposon into Brucella abortusQ44949619
Hierarchical gene regulatory systems arising from fortuitous gene associations: controlling quorum sensing by the opine regulon in AgrobacteriumQ47957710
Spacing of the -10 and -35 regions in the tac promoter. Effect on its in vivo activity.Q48380581
P433issue16
P407language of work or nameEnglishQ1860
P921main subjectquorum sensingQ905754
P304page(s)5053-5062
P577publication date2008-07-07
P1433published inApplied and Environmental MicrobiologyQ4781593
P1476titleBroad-host-range expression vectors with tightly regulated promoters and their use to examine the influence of TraR and TraM expression on Ti plasmid quorum sensing
P478volume74

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cites work (P2860)
Q99726743A High-efficacy CRISPRi System for Gene Function Discovery in Zymomonas mobilis
Q36504514A LuxR-type repressor of Burkholderia cenocepacia inhibits transcription via antiactivation and is inactivated by its cognate acylhomoserine lactone
Q99365552A central role for the transcriptional regulator VtlR in small RNA-mediated gene regulation in Agrobacterium tumefaciens
Q28607592A distinct pathway for tetrahymanol synthesis in bacteria
Q92709037A high-throughput system to identify inhibitors of Candidatus Liberibacter asiaticus transcription regulators
Q90330462A novel 3-oxoacyl-ACP reductase (FabG3) is involved in the xanthomonadin biosynthesis of Xanthomonas campestris pv. campestris
Q89951171A novel way to synthesize pantothenate in bacteria involves β-alanine synthase present in uracil degradation pathway
Q37643430A signaling pathway involving the diguanylate cyclase CelR and the response regulator DivK controls cellulose synthesis in Agrobacterium tumefaciens
Q36739341A solo luxI-type gene directs acylhomoserine lactone synthesis and contributes to motility control in the marine sponge symbiont Ruegeria sp. KLH11
Q33842724A unique regulator controls the activation threshold of quorum-regulated genes in Pseudomonas aeruginosa
Q45805866A widely conserved molecular switch controls quorum sensing and symbiosis island transfer in Mesorhizobium loti through expression of a novel antiactivator
Q38718421Absence of the Polar Organizing Protein PopZ Results in Reduced and Asymmetric Cell Division in Agrobacterium tumefaciens.
Q42094089Acetoacetyl-CoA synthetase activity is controlled by a protein acetyltransferase with unique domain organization in Streptomyces lividans
Q34056929Agrobacterium tumefaciens exoR controls acid response genes and impacts exopolysaccharide synthesis, horizontal gene transfer, and virulence gene expression
Q43050667An inner membrane cytochrome required only for reduction of high redox potential extracellular electron acceptors
Q30604477Analysis of a taurine-dependent promoter in Sinorhizobium meliloti that offers tight modulation of gene expression
Q35832790Analysis of the FnrL regulon in Rhodobacter capsulatus reveals limited regulon overlap with orthologues from Rhodobacter sphaeroides and Escherichia coli.
Q35096466Antiparallel and interlinked control of cellular iron levels by the Irr and RirA regulators of Agrobacterium tumefaciens
Q30152624AraC-like transcriptional activator CuxR binds c-di-GMP by a PilZ-like mechanism to regulate extracellular polysaccharide production
Q28822597Assembly and transfer of tripartite integrative and conjugative genetic elements
Q37257344Bacterial argonaute samples the transcriptome to identify foreign DNA
Q64448309Bacteriophage-associated genes responsible for the widely divergent phenotypes of variants of Burkholderia pseudomallei strain MSHR5848
Q34988036Bi-modal distribution of the second messenger c-di-GMP controls cell fate and asymmetry during the caulobacter cell cycle
Q28675545Brucella BioR regulator defines a complex regulatory mechanism for bacterial biotin metabolism
Q92766605Brucella Periplasmic Protein EipB Is a Molecular Determinant of Cell Envelope Integrity and Virulence
Q35644929Candidatus Frankia Datiscae Dg1, the Actinobacterial Microsymbiont of Datisca glomerata, Expresses the Canonical nod Genes nodABC in Symbiosis with Its Host Plant
Q92402114Cell Aggregation and Aerobic Respiration Are Important for Zymomonas mobilis ZM4 Survival in an Aerobic Minimal Medium
Q36229443Cell Cycle Constraints and Environmental Control of Local DNA Hypomethylation in α-Proteobacteria
Q35632794Cell Cycle Control by the Master Regulator CtrA in Sinorhizobium meliloti.
Q47095068Characterization of the CrbS/R Two-Component System in Pseudomonas fluorescens Reveals a New Set of Genes under Its Control and a DNA Motif Required for CrbR-Mediated Transcriptional Activation
Q64134484CitAB Two-Component System-Regulated Citrate Utilization Contributes to Vibrio cholerae Competitiveness with the Gut Microbiota
Q43928694Construction of a tightly-controlled expression system for use in Pseudomonas
Q92025938Control of nitrogen fixation in bacteria that associate with cereals
Q34599268Coordination of division and development influences complex multicellular behavior in Agrobacterium tumefaciens
Q40433323CtrA controls cell division and outer membrane composition of the pathogen Brucella abortus
Q36674888DTDP-rhamnosyl transferase RfbF, is a newfound receptor-related regulatory protein for phage phiYe-F10 specific for Yersinia enterocolitica serotype O:3.
Q35185420Determinants within the C-terminal domain of Streptomyces lividans acetyl-CoA synthetase that block acetylation of its active site lysine in vitro by the protein acetyltransferase (Pat) enzyme
Q36074478Development of an IPTG inducible expression vector adapted for Bacteroides fragilis
Q37430801Discovery of a cAMP deaminase that quenches cyclic AMP-dependent regulation
Q36736529Discrete Responses to Limitation for Iron and Manganese in Agrobacterium tumefaciens: Influence on Attachment and Biofilm Formation.
Q35178367Diversity and functional analysis of luxS genes in vibrios from marine sponges Mycale laxissima and Ircinia strobilina
Q36895722Dynamic FtsA and FtsZ localization and outer membrane alterations during polar growth and cell division in Agrobacterium tumefaciens
Q50110380Effective removal of a range of Ti/Ri plasmids using a pBBR1-type vector having a repABC operon and a lux reporter system.
Q36362469Effects of engineered Sinorhizobium meliloti on cytokinin synthesis and tolerance of alfalfa to extreme drought stress
Q37713231Enhanced interaction of Vibrio cholerae virulence regulators TcpP and ToxR under oxygen-limiting conditions
Q41023328Epigenetics knocks on synthetic biology's door.
Q37727586Functional Characterization and Conditional Regulation of the Type VI Secretion System in Vibrio fluvialis
Q42362561Functional Characterization of Triclosan-Resistant Enoyl-acyl-carrier Protein Reductase (FabV) in Pseudomonas aeruginosa
Q88985427Functional assignment of multiple catabolic pathways for D-apiose
Q36300722Functional metagenomics reveals novel β-galactosidases not predictable from gene sequences
Q33976662GIL, a new c-di-GMP-binding protein domain involved in regulation of cellulose synthesis in enterobacteria.
Q91996007GROWTH POLE RING protein forms a 200-nm-diameter ring structure essential for polar growth and rod shape in Agrobacterium tumefaciens
Q37237699Genetic analysis of Agrobacterium tumefaciens unipolar polysaccharide production reveals complex integrated control of the motile-to-sessile switch
Q38820564Genome sequencing-assisted identification and the first functional validation of N-acyl-homoserine-lactone synthases from the Sphingomonadaceae family.
Q35160857Genome-wide profiling of Hfq-binding RNAs uncovers extensive post-transcriptional rewiring of major stress response and symbiotic regulons in Sinorhizobium meliloti.
Q41965400Glucose uptake in Azotobacter vinelandii occurs through a GluP transporter that is under the control of the CbrA/CbrB and Hfq-Crc systems.
Q46458492Heme binding and peroxidase activity of a secreted minicatalase
Q42190199HmuP is a coactivator of Irr-dependent expression of heme utilization genes in Bradyrhizobium japonicum
Q28709564Identification and characterization of Rhodopseudomonas palustris TIE-1 hopanoid biosynthesis mutants
Q33847666Identification of the in vivo function of the high-efficiency D-mannonate dehydratase in Caulobacter crescentus NA1000 from the enolase superfamily.
Q34837547Independent activity of the homologous small regulatory RNAs AbcR1 and AbcR2 in the legume symbiont Sinorhizobium meliloti.
Q55044886Integration Host Factor Modulates the Expression and Function of T6SS2 in Vibrio fluvialis.
Q47245741Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth.
Q30771916Loss of PodJ in Agrobacterium tumefaciens Leads to Ectopic Polar Growth, Branching, and Reduced Cell Division
Q43620250Loss of PopZ At activity in Agrobacterium tumefaciens by Deletion or Depletion Leads to Multiple Growth Poles, Minicells, and Growth Defects
Q40949451Maf-dependent bacterial flagellin glycosylation occurs before chaperone binding and flagellar T3SS export
Q35866012Members of the PpaA/AerR Antirepressor Family Bind Cobalamin
Q38842114Membrane and core periplasmic Agrobacterium tumefaciens virulence Type IV secretion system components localize to multiple sites around the bacterial perimeter during lateral attachment to plant cells.
Q49678053Microsymbiont discrimination mediated by a host-secreted peptide in Medicago truncatula.
Q36048919Mini-Tn7 Insertion in an Artificial attTn7 Site Enables Depletion of the Essential Master Regulator CtrA in the Phytopathogen Agrobacterium tumefaciens
Q37435032Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp.
Q28818660Modularity and determinants of a (bi-)polarization control system from free-living and obligate intracellular bacteria
Q64891903Modulation of CrbS-Dependent Activation of the Acetate Switch in Vibrio cholerae.
Q27677279Molecular Structure and Function of the Novel BrnT/BrnA Toxin-Antitoxin System of Brucella abortus
Q42934570Molecular cloning of Reteplase and its expression in E. coli using tac promoter
Q47176722Mutations in Escherichia coli polyphosphate kinase that lead to dramatically increased in vivo polyphosphate levels
Q37512419Negative Regulation of Ectoine Uptake and Catabolism in Sinorhizobium meliloti: Characterization of the EhuR Gene
Q38848330New vectors for chromosomal integration enable high-level constitutive or inducible magnetosome expression of fusion proteins in Magnetospirillum gryphiswaldense
Q54203048Novel Xanthomonas campestris Long-Chain-Specific 3-Oxoacyl-Acyl Carrier Protein Reductase Involved in Diffusible Signal Factor Synthesis.
Q35172326Novel pseudotaxis mechanisms improve migration of straight-swimming bacterial mutants through a porous environment.
Q42621381Novel toxin-antitoxin system composed of serine protease and AAA-ATPase homologues determines the high level of stability and incompatibility of the tumor-inducing plasmid pTiC58.
Q90929491Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus
Q46546817OxyR-regulated catalase activity is critical for oxidative stress resistance, nodulation and nitrogen fixation in Azorhizobium caulinodans
Q33709570Peptidoglycan synthesis machinery in Agrobacterium tumefaciens during unipolar growth and cell division
Q90402689Periplasmic protein EipA determines envelope stress resistance and virulence in Brucella abortus
Q41355922Polar Organizing Protein PopZ Is Required for Chromosome Segregation in Agrobacterium tumefaciens
Q36079170PopZ identifies the new pole, and PodJ identifies the old pole during polar growth in Agrobacterium tumefaciens
Q47243718Primary Characterization of Small RNAs in Symbiotic Nitrogen-Fixing Bacteria.
Q36718100Profligate biotin synthesis in α-proteobacteria - a developing or degenerating regulatory system?
Q64945550Programmable DNA cleavage by Ago nucleases from mesophilic bacteria Clostridium butyricum and Limnothrix rosea.
Q33860256Pseudomonas putida F1 has multiple chemoreceptors with overlapping specificity for organic acids
Q89494979Quorum Sensing-Independent Cellulase-Sensitive Pellicle Formation Is Critical for Colonization of Burkholderia glumae in Rice Plants
Q37643373Quorum-dependent mannopine-inducible conjugative transfer of an Agrobacterium opine-catabolic plasmid
Q38850761RNase E affects the expression of the acyl-homoserine lactone synthase gene sinI in Sinorhizobium meliloti
Q58698346RNase III: Catalytic Features and Impact on Symbiosis
Q35754601Ralstonia solanacearum RSp0194 Encodes a Novel 3-Keto-Acyl Carrier Protein Synthase III
Q36277152Regulatable vectors for environmental gene expression in Alphaproteobacteria
Q41674655Regulation of Polyhydroxybutyrate Accumulation in Sinorhizobium meliloti by the Trans-Encoded Small RNA MmgR.
Q46264177Regulation of cysteine residues in LsrB proteins from Sinorhizobium meliloti under free-living and symbiotic oxidative stress
Q34632437Regulation of the phototrophic iron oxidation (pio) genes in Rhodopseudomonas palustris TIE-1 is mediated by the global regulator, FixK.
Q37837167Regulatory (pan-)genome of an obligate intracellular pathogen in the PVC superphylum.
Q40317191Restoring virulence to mutants lacking subunits of multiprotein machines: functional complementation of a Brucella virB5 mutant.
Q30363384Role and Regulation of ACC Deaminase Gene in Sinorhizobium meliloti: Is It a Symbiotic, Rhizospheric or Endophytic Gene?
Q34388787Role of specific quorum-sensing signals in the regulation of exopolysaccharide II production within Sinorhizobium meliloti spreading colonies
Q40646972Scarless Genome Editing and Stable Inducible Expression Vectors for Geobacter sulfurreducens
Q36296785Seeing red; the development of pON.mCherry, a broad-host range constitutive expression plasmid for Gram-negative bacteria
Q52346317Sequential induction of three recombination directionality factors directs assembly of tripartite integrative and conjugative elements.
Q58785287Seven-transmembrane receptor protein RgsP and cell wall-binding protein RgsM promote unipolar growth in Rhizobiales
Q47336510Sinorhizobium meliloti glutathione reductase is required for both redox homeostasis and symbiosis
Q46599290Spatiotemporal choreography of chromosome and megaplasmids in the Sinorhizobium meliloti cell cycle.
Q37248302Spermidine Inversely Influences Surface Interactions and Planktonic Growth in Agrobacterium tumefaciens.
Q41357740Stand-Alone EAL Domain Proteins Form a Distinct Subclass of EAL Proteins Involved in Regulation of Cell Motility and Biofilm Formation in Enterobacteria.
Q35865584Structural asymmetry in a conserved signaling system that regulates division, replication, and virulence of an intracellular pathogen
Q90597981Structure of ribosome-bound azole-modified peptide phazolicin rationalizes its species-specific mode of bacterial translation inhibition
Q55376074Successful Transfer of a Model T-DNA Plasmid to E. coli Revealed Its Dependence on Recipient RecA and the Preference of VirD2 Relaxase for Eukaryotes Rather Than Bacteria as Recipients.
Q37589897Synthesis of arborane triterpenols by a bacterial oxidosqualene cyclase
Q35139186TdaA regulates Tropodithietic acid synthesis by binding to the tdaC promoter region
Q39671652The Aeromonas caviae AHA0618 gene modulates cell length and influences swimming and swarming motility.
Q47606354The Agrobacterium tumefaciens CheY-like protein ClaR regulates biofilm formation
Q43192635The BlcC (AttM) lactonase of Agrobacterium tumefaciens does not quench the quorum-sensing system that regulates Ti plasmid conjugative transfer
Q34794056The CckA-ChpT-CtrA phosphorelay system is regulated by quorum sensing and controls flagellar motility in the marine sponge symbiont Ruegeria sp. KLH11.
Q34056658The Ctp type IVb pilus locus of Agrobacterium tumefaciens directs formation of the common pili and contributes to reversible surface attachment
Q42142682The DivJ, CbrA and PleC system controls DivK phosphorylation and symbiosis in Sinorhizobium meliloti
Q96431442The Leader Peptide peTrpL Forms Antibiotic-Containing Ribonucleoprotein Complexes for Posttranscriptional Regulation of Multiresistance Genes
Q38933574The Ralstonia solanacearum effector RipAK suppresses plant hypersensitive response by inhibiting the activity of host catalases.
Q42315045The RegA regulon exhibits variability in response to altered growth conditions and differs markedly between Rhodobacter species
Q37335354The Sinorhizobium meliloti ntrX gene is involved in succinoglycan production, motility, and symbiotic nodulation on alfalfa
Q37715985The Vitamin B12-Dependent Photoreceptor AerR Relieves Photosystem Gene Repression by Extending the Interaction of CrtJ with Photosystem Promoters
Q28681751The general stress response factor EcfG regulates expression of the C-2 hopanoid methylase HpnP in Rhodopseudomonas palustris TIE-1
Q40562890The identification of an integral membrane, cytochrome c urate oxidase completes the catalytic repertoire of a therapeutic enzyme
Q42177017The quorum-sensing protein TraR of Agrobacterium tumefaciens is susceptible to intrinsic and TraM-mediated proteolytic instability
Q36429437The repABC Plasmids with Quorum-Regulated Transfer Systems in Members of the Rhizobiales Divide into Two Structurally and Separately Evolving Groups
Q47216710The ropAe gene encodes a porin-like protein involved in copper transit in Rhizobium etli CFN42.
Q90006426The transcription factors ActR and SoxR differentially affect the phenazine tolerance of Agrobacterium tumefaciens
Q42362248Toward the identification of a type I toxin-antitoxin system in the plasmid DNA of dairy Lactobacillus rhamnosus.
Q93143179Transcription attenuation-derived small RNA rnTrpL regulates tryptophan biosynthesis gene expression in trans
Q34058418Transcriptional regulator LsrB of Sinorhizobium meliloti positively regulates the expression of genes involved in lipopolysaccharide biosynthesis
Q37232899Xanthomonas campestris FabH is required for branched-chain fatty acid and DSF-family quorum sensing signal biosynthesis
Q101403017α-proteobacteria synthesize biotin precursor pimeloyl-ACP using BioZ 3-ketoacyl-ACP synthase and lysine catabolism

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