The effect of primer-template mismatches on the detection and quantification of nucleic acids using the 5' nuclease assay

scientific article published on 30 November 2009

The effect of primer-template mismatches on the detection and quantification of nucleic acids using the 5' nuclease assay is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.2353/JMOLDX.2010.090035
P932PMC publication ID2797725
P698PubMed publication ID19948821
P5875ResearchGate publication ID40041920

P50authorSuzan D PasQ56871934
Martin SchuttenQ115215207
P2093author name stringTed H M Mes
Jolanda Voermans
Ralph Stadhouders
Jeer Anber
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The effects of sequence length and oligonucleotide mismatches on 5' exonuclease assay efficiencyQ34368727
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The effects of internal primer-template mismatches on RT-PCR: HIV-1 model studiesQ34625250
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Polymerase chain reaction strategyQ35330788
Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studiesQ35829749
Extension of base mispairs by Taq DNA polymerase: implications for single nucleotide discrimination in PCRQ35927120
The use of real-time PCR methods in DNA sequence variation analysisQ36267022
Misincorporation and mispaired primer extension by human immunodeficiency virus reverse transcriptaseQ38304218
Influence of preassay and sequence variations on viral load determination by a multiplex real-time reverse transcriptase-polymerase chain reaction for feline immunodeficiency virusQ38304269
Reactions at the polymerase active site that contribute to the fidelity of Escherichia coli DNA polymerase I (Klenow fragment).Q38323757
Ultrasensitive monitoring of HIV-1 viral load by a low-cost real-time reverse transcription-PCR assay with internal control for the 5' long terminal repeat domainQ38978220
Nucleotide analogs facilitate base conversion with 3' mismatch primersQ39727783
Advances in quantitative PCR technology: 5' nuclease assaysQ41721416
Base mispair extension kinetics. Binding of avian myeloblastosis reverse transcriptase to matched and mismatched base pair terminiQ41787453
Quantification of the detrimental effect of a single primer-template mismatch by real-time PCR using the 16S rRNA gene as an example.Q43064023
Quantification of the newly detected lamivudine resistant YSDD variants of Hepatitis B virus using molecular beaconsQ44288679
Base mispair extension kinetics. Comparison of DNA polymerase alpha and reverse transcriptaseQ46193418
Influence of DNA structure on DNA polymerase beta active site function: extension of mutagenic DNA intermediates.Q53639781
Sequence variation in primer targets affects the accuracy of viral quantitative PCR.Q54644048
Specificity, efficiency, and fidelity of PCRQ72268477
P433issue1
P304page(s)109-117
P577publication date2009-11-30
P1433published inThe Journal of Molecular DiagnosticsQ7743603
P1476titleThe effect of primer-template mismatches on the detection and quantification of nucleic acids using the 5' nuclease assay
P478volume12

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