Inactivation of Na,K-ATPase following Co(NH3)4ATP binding at a low affinity site in the protomeric enzyme unit

scientific article published on 18 February 2003

Inactivation of Na,K-ATPase following Co(NH3)4ATP binding at a low affinity site in the protomeric enzyme unit is …
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scholarly articleQ13442814

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P356DOI10.1074/JBC.M211128200
P698PubMed publication ID12591931

P50authorDouglas G. WardQ39062695
P2093author name stringJosé D Cavieres
P2860cites workMeasurement of protein using bicinchoninic acidQ26778491
Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 A resolutionQ27625023
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Solubilized alpha beta Na,K-ATPase remains protomeric during turnover yet shows apparent negative cooperativity toward ATPQ36350106
An evaluation of active enzyme centrifugation as a zonal and boundary technique by the analysis of simulated dataQ36652224
Replacement of several single amino acid side chains exposed to the inside of the ATP-binding pocket induces different extents of affinity change in the high and low affinity ATP-binding sites of rat Na/K-ATPaseQ38364012
Purification and characterization of (Na+, K+)-ATPase. V. Conformational changes in the enzyme. Transitions between the Na-form and the K-form studied with tryptic digestion as a toolQ39745573
Occlusion of rubidium ions by the sodium-potassium pump: its implications for the mechanism of potassium transportQ41554966
Purification and characterization of (Na+ plus K+ )-ATPase. 3. Purification from the outer medulla of mammalian kidney after selective removal of membrane components by sodium dodecylsulphateQ45249662
Competitive binding of ATP and the fluorescent substrate analogue 2',3'-O-(2,4,6-trinitrophenylcyclohexadienylidine) adenosine 5'-triphosphate to the gastric H+,K+-ATPase: evidence for two classes of nucleotide sitesQ45277289
Simultaneous binding of phosphate and TNP-ADP to FITC-modified NA+,K(+)-ATPaseQ46272494
Photoaffinity labeling of the active site of the Na+/K(+)-ATPase with 4-azido-2-nitrophenyl phosphateQ46536218
Identification of an amino acid in the ATP binding site of Na+/K(+)-ATPase after photochemical labeling with 8-azido-ATP.Q46782777
Photoinactivation of fluorescein isothiocyanate-modified Na,K-ATPase by 2'(3')-O-(2,4,6-trinitrophenyl)8-azidoadenosine 5'-diphosphate. Abolition of E1 and E2 partial reactions by sequential block of high and low affinity nucleotide sitesQ48034678
Fast reversal of the initial reaction steps of the plasma membrane (Ca2+ + Mg2+)-ATPase.Q52596469
Molecular characterization of proteins in detergent solutions.Q52907053
Soluble and enzymatically stable (Na+ + K+)-ATPase from mammalian kidney consisting predominantly of protomer alpha beta-units. Preparation, assay and reconstitution of active Na+, K+ transportQ67292318
Phosphate binding and ATP-binding sites coexist in Na+/K(+)-transporting ATPase, as demonstrated by the inactivating MgPO4 complex analogue Co(NH3)4PO4Q67913531
Properties of a phosphorylated protein as a reaction intermediate of Na+-K+ sensitive ATPaseQ68402600
Existence of a low-affinity ATP-binding site in the unphosphorylated Ca2(+)-ATPase of sarcoplasmic reticulum vesicles: evidence from binding of 2',3'-O-(2,4,6-trinitrocyclohexadienylidene)-[3H]AMP and -[3H]ATPQ68430470
How do MgATP analogues differentially modify high-affinity and low-affinity ATP binding sites of Na+/K(+)-ATPase?Q68472218
Shift to the Na+ form of Na+/K+-transporting ATPase due to modification of the low-affinity ATP-binding site by Co(NH3)4ATPQ69087324
Minimum enzyme unit for Na+/K+-ATPase is the alpha beta-protomer. Determination by low-angle laser light scattering photometry coupled with high-performance gel chromatography for substantially simultaneous measurement of ATPase activity and moleculQ69353692
Demonstration of cooperating alpha subunits in working (Na+ + K+)-ATPase by the use of the MgATP complex analogue cobalt tetrammine ATPQ69809285
Substrate sites of the (Na+ + K+)-ATPase: pertinence of the adenine and fluorescein binding sitesQ69818778
Characteristics of 3-O-methylfluorescein phosphate hydrolysis by the (Na+ + K+)-ATPaseQ69847341
Thermoinactivation and aggregation of alpha beta units in soluble and membrane-bound (Na,K)-ATPaseQ70141857
Binding of 2'(3')-O-(2,4-6-trinitrophenyl) ADP to soluble alpha beta protomers of Na, K-ATPase modified with fluorescein isothiocyanate. Evidence for two distinct nucleotide sitesQ71119838
Na+/K(+)-ATPase with a blocked E1ATP site still allows backdoor phosphorylation of the E2ATP siteQ71735147
ADP supports ouabain-sensitive K-K exchange in human red blood cellsQ72066947
The role of (alpha beta) protomer interaction in determining functional characteristics of red cell Na,K-ATPaseQ72076960
Identification of a labelled peptide after stoicheiometric reaction of fluorescein isothiocyanate with the Ca2+ -dependent adenosine triphosphatase of sarcoplasmic reticulumQ72769416
Characterization of conformational changes in (Na,K) ATPase labeled with fluorescein at the active siteQ72887395
Soluble and active renal Na,K-ATPase with maximum protein molecular mass 170,000 ± 9,000 daltons; formation of larger units by secondary aggregationQ72901189
Na+, K+-ATPase: evidence for the binding of ATP to the phosphoenzymeQ72928676
Combined effects of ATP and phosphate on rubidium exchange mediated by Na-K-ATPase reconstituted into phospholipid vesiclesQ72960176
Modification of the E1ATP binding site of Na+/K(+)-ATPase by the chromium complex of adenosine 5'-[beta,gamma-methylene]triphosphate blocks the overall reaction but not the partial activities of the E2 conformationQ72966401
Ligands presumed to label high affinity and low affinity ATP binding sites do not interact in an (alpha beta)2 diprotomer in duck nasal gland Na+,K+-ATPase, nor Do the sites coexist in native enzymeQ73834581
Oligomeric structure of solubilized Na+/K(+)-ATPase linked to E1/E2 conformationQ73956816
Erythrosin isothiocyanate selectively labels lysine464 within an ATP-protectable binding site on the Ca-ATPase in skeletal sarcoplasmic reticulum membranesQ74523844
Affinity labeling of two nucleotide sites on Na,K-ATPase using 2'(3')-O-(2,4,6-trinitrophenyl)8-azidoadenosine 5'-[alpha-32P]diphosphate (TNP-8N3-[alpha-32P]ADP) as a photoactivatable probe. Label incorporation before and after blocking the high affQ77631075
Preparation of Na+,K+-ATPase with near maximal specific activity and phosphorylation capacity: evidence that the reaction mechanism involves all of the sitesQ77849922
ATP hydrolysis associated with an uncoupled sodium flux through the sodium pump: evidence for allosteric effects of intracellular ATP and extracellular sodiumQ80320175
P433issue17
P407language of work or nameEnglishQ1860
P1104number of pages10
P304page(s)14688-14697
P577publication date2003-02-18
P1433published inJournal of Biological ChemistryQ867727
P1476titleInactivation of Na,K-ATPase following Co(NH3)4ATP binding at a low affinity site in the protomeric enzyme unit
P478volume278

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cites work (P2860)
Q37395857Mechanism of allosteric effects of ATP on the kinetics of P-type ATPases
Q44605200The Amino Acid Sequence 442GDASE446 in Na/K-ATPase Is an Important Motif in Forming the High and Low Affinity ATP Binding Pockets

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