Example of real-time quantitative reverse transcription-PCR (Q-RT-PCR) analysis of bacterial gene expression during mammalian infection: Borrelia burgdorferi in mouse tissues

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Example of real-time quantitative reverse transcription-PCR (Q-RT-PCR) analysis of bacterial gene expression during mammalian infection: Borrelia burgdorferi in mouse tissues is …
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scholarly articleQ13442814

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P356DOI10.1002/9780471729259.MC01D03S00
P698PubMed publication ID18770562

P2093author name stringJennifer C Miller
P2860cites workTemporal pattern of Borrelia burgdorferi p21 expression in ticks and the mammalian hostQ37364561
A new animal model for studying Lyme disease spirochetes in a mammalian host-adapted stateQ37382131
RpoS is not central to the general stress response in Borrelia burgdorferi but does control expression of one or more essential virulence determinantsQ37582867
Quantitative analysis of Borrelia burgdorferi gene expression in naturally (tick) infected mouse strainsQ47688868
Immunological and genetic characterization of Borrelia burgdorferi BapA and EppA proteinsQ47941528
Analysis of Borrelia burgdorferi gene expression during life cycle phases of the tick vector Ixodes scapularis.Q52591505
Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferiQ22122357
Induction of an outer surface protein on Borrelia burgdorferi during tick feedingQ24564198
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform ExtractionQ25938986
A bacterial genome in flux: the twelve linear and nine circular extrachromosomal DNAs in an infectious isolate of the Lyme disease spirochete Borrelia burgdorferiQ28144621
Identification of 11 pH-regulated genes in Borrelia burgdorferi localizing to linear plasmidsQ30964822
bdrF2 of Lyme disease spirochetes is coexpressed with a series of cytoplasmic proteins and is produced specifically during early infectionQ33553653
Expression and immunological analysis of the plasmid-borne mlp genes of Borrelia burgdorferi strain B31.Q33598074
A second allele of eppA in Borrelia burgdorferi strain B31 is located on the previously undetected circular plasmid cp9-2Q33994874
Regulation of OspE-related, OspF-related, and Elp lipoproteins of Borrelia burgdorferi strain 297 by mammalian host-specific signalsQ34007612
Distinct regulatory pathways control expression of Borrelia burgdorferi infection-associated OspC and Erp surface proteinsQ34008137
Demonstration of the genetic stability and temporal expression of select members of the lyme disease spirochete OspF protein family during infection in miceQ34008518
Borrelia burgdorferi RevA antigen is a surface-exposed outer membrane protein whose expression is regulated in response to environmental temperature and pHQ34008806
Borrelia burgdorferi population kinetics and selected gene expression at the host-vector interfaceQ34126051
Environmental regulation and differential production of members of the Bdr protein family of Borrelia burgdorferiQ34258625
Borrelia burgdorferi genes selectively expressed in the infected hostQ34317419
OspE-related, OspF-related, and Elp lipoproteins are immunogenic in baboons experimentally infected with Borrelia burgdorferi and in human lyme disease patientsQ34439525
Temporal analysis of Borrelia burgdorferi Erp protein expression throughout the mammal-tick infectious cycleQ34945715
Regulation of expression of the paralogous Mlp family in Borrelia burgdorferiQ35802176
Borrelia burgdorferi population dynamics and prototype gene expression during infection of immunocompetent and immunodeficient miceQ35802349
The plasmids of Borrelia burgdorferi: essential genetic elements of a pathogenQ35999642
Evidence for in vivo but not in vitro expression of a Borrelia burgdorferi outer surface protein F (OspF) homologueQ36812377
P921main subjectLyme diseaseQ201989
Borrelia burgdorferiQ2723634
P304page(s)Unit 1D.3
P577publication date2005-10-01
P1433published inCurrent Protocols in MicrobiologyQ26842441
P1476titleExample of real-time quantitative reverse transcription-PCR (Q-RT-PCR) analysis of bacterial gene expression during mammalian infection: Borrelia burgdorferi in mouse tissues
P478volumeChapter 1D

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cites work (P2860)
Q37256541Borrelia burgdorferi RevA antigen binds host fibronectin
Q88310828Borrelia burgdorferi SpoVG DNA- and RNA-Binding Protein Modulates the Physiology of the Lyme Disease Spirochete
Q36906444Borrelia burgdorferi complement regulator-acquiring surface proteins (BbCRASPs): Expression patterns during the mammal-tick infection cycle
Q36152815Borrelia burgdorferi cp32 BpaB modulates expression of the prophage NucP nuclease and SsbP single-stranded DNA-binding protein
Q35739170BpaB and EbfC DNA-binding proteins regulate production of the Lyme disease spirochete's infection-associated Erp surface proteins
Q36581174Changes in bacterial growth rate govern expression of the Borrelia burgdorferi OspC and Erp infection-associated surface proteins
Q35947203Coordinated expression of Borrelia burgdorferi complement regulator-acquiring surface proteins during the Lyme disease spirochete's mammal-tick infection cycle
Q47793653Detection of Bioluminescent Borrelia burgdorferi from In Vitro Cultivation and During Murine Infection
Q36211772EbfC (YbaB) is a new type of bacterial nucleoid-associated protein and a global regulator of gene expression in the Lyme disease spirochete
Q35612948Intracellular Concentrations of Borrelia burgdorferi Cyclic Di-AMP Are Not Changed by Altered Expression of the CdaA Synthase
Q37253168Posttranscriptional self-regulation by the Lyme disease bacterium's BpuR DNA/RNA-binding protein
Q36153900RNA-Seq of Borrelia burgdorferi in Multiple Phases of Growth Reveals Insights into the Dynamics of Gene Expression, Transcriptome Architecture, and Noncoding RNAs
Q93034876The Lyme disease spirochete's BpuR DNA/RNA-binding protein is differentially expressed during the mammal-tick infectious cycle, which affects translation of the SodA superoxide dismutase
Q34697832Transcriptional regulation of the Borrelia burgdorferi antigenically variable VlsE surface protein
Q64907378Transcriptomic insights on the virulence-controlling CsrA, BadR, RpoN, and RpoS regulatory networks in the Lyme disease spirochete.

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