Quantification of G protein Gaalphas subunit splice variants in different human tissues and cells using pyrosequencing

scientific article published in January 2005

Quantification of G protein Gaalphas subunit splice variants in different human tissues and cells using pyrosequencing is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.3727/000000005783992124
P932PMC publication ID6009110
P698PubMed publication ID15892449

P2093author name stringHans Hauner
Dobromir Dobrev
Holger Nückel
Andreas Eisenhardt
Winfried Siffert
Ulrich H Frey
I E Sandalcioglu
Iris Manthey
Karl Worm
P2860cites workDifferential localization and development-dependent expression of G-protein subunits, Go alpha and G beta, in rabbit heart.Q52200771
Developmental changes in adenylyl cyclase and GTP binding proteins in brown fat.Q52236070
Alteration in the protein components of catecholamine-sensitive adenylate cyclase during maturation of rat reticulocytesQ52292372
A simplified ribonuclease protection assayQ67804729
Age-related alterations in adenylyl cyclase system of rat heartsQ68251183
Purification of the major GTP-binding proteins from human placental membranesQ70284484
Differences in transcription and translation of long and short Gs alpha, the stimulatory G-protein, in human atriumQ70867082
Differential coupling of glucagon and beta-adrenergic receptors with the small and large forms of the stimulatory G proteinQ71522829
Prolonged exposure of hamsters to cold changes the levels of G proteins in brown adipose tissue plasma membranesQ71852386
Single-nucleotide polymorphism analysis by pyrosequencingQ73775865
G-Protein beta(3)-subunit 825T allele is associated with enhanced human atrial inward rectifier potassium currentsQ74131870
Assessing allele frequencies of single nucleotide polymorphisms in DNA pools by pyrosequencing technologyQ74133504
Estimation of single nucleotide polymorphism allele frequency in DNA pools by using PyrosequencingQ74320712
The long (Gs(alpha)-L) and short (Gs(alpha)-S) variants of the stimulatory guanine nucleotide-binding protein. Do they behave in an identical way?Q74538366
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodQ25938999
A sequencing method based on real-time pyrophosphateQ28279786
Isolation and characterization of the human Gs alpha geneQ28646194
Alternative pre-mRNA splicing: the logic of combinatorial controlQ29615087
Characterization of Gs alpha mRNA transcripts in primary cultures of rat brain astrocytesQ30838378
The regulatory component of adenylate cyclase. Purification and propertiesQ34252831
An overview of real-time quantitative PCR: applications to quantify cytokine gene expressionQ34532446
Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteinsQ35585507
Molecular basis for two forms of the G protein that stimulates adenylate cyclaseQ36425371
Human cDNA clones for four species of G alpha s signal transduction proteinQ36426004
Alternative splicing of the adenylyl cyclase stimulatory G-protein G alpha(s) is regulated by SF2/ASF and heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) and involves the use of an unusual TG 3'-splice SiteQ38292377
Human myometrial G alpha s-small (with serine) and Gs-large (with serine) messenger ribonucleic acid splice variants promote the increased expression of 46- and 54-kilodalton G alpha s protein isoforms in pregnancy and their down-regulation during lQ38360919
Quantification of splice variants using real-time PCRQ39095908
Preterm labour: a pharmacological challengeQ40443938
G-protein betagamma-subunits contribute to the coupling specificity of the beta2-adrenergic receptor to G(s).Q40746352
Enhanced G protein activation in immortalized lymphoblasts from patients with essential hypertension.Q41318399
A faster ribonuclease protection assayQ41593318
Increase in Gs and cyclic AMP generation in HIT cells. Evidence that the 45-kDa alpha-subunit of Gs has greater functional activity than the 52-kDa alpha-subunitQ41758220
Phosphorylation of the spliced variant forms of the recombinant stimulatory guanine-nucleotide-binding regulatory protein (Gs alpha) by protein kinase C.Q41911508
Different effects of Gsalpha splice variants on beta2-adrenoreceptor-mediated signaling. The Beta2-adrenoreceptor coupled to the long splice variant of Gsalpha has properties of a constitutively active receptorQ42060564
Selective stabilization of the high affinity binding conformation of glucagon receptor by the long splice variant of Galpha(s).Q42487424
Effects of the G-protein beta3 subunit 825T allele on adipogenesis and lipolysis in cultured human preadipocytes and adipocytesQ42526979
Attenuation of Gs alpha coupling efficiency in brown-adipose-tissue plasma membranes from cold-acclimated hamstersQ42812822
Monovalent anions differentially modulate coupling of the beta2-adrenoceptor to G(s)alpha splice variantsQ43675618
Differential expression of low molecular weight form of Gs-alpha in neostriatum and cerebellum: correlation with expression of calmodulin-independent adenylyl cyclaseQ47444288
Phosphorylation of the recombinant spliced variants of the alpha-sub-unit of the stimulatory guanine-nucleotide binding regulatory protein (Gs) by the catalytic sub-unit of protein kinase AQ48453749
Splicing Pattern of Gs? mRNA in Human and Rat BrainQ48662412
P4510describes a project that usespyrosequencingQ2342248
P433issue2
P921main subjectpyrosequencingQ2342248
P304page(s)69-81
P577publication date2005-01-01
P1433published inGene ExpressionQ15755770
P1476titleQuantification of G protein Gaalphas subunit splice variants in different human tissues and cells using pyrosequencing
P478volume12

Reverse relations

cites work (P2860)
Q51710260Amelogenin sex determination by pyrosequencing of short PCR products.
Q41949383Analysis of splicing patterns by pyrosequencing.
Q43253649Comparison of methods for quantification of subtle splice variants
Q42594172High resolution analysis of the human transcriptome: detection of extensive alternative splicing independent of transcriptional activity
Q36062526Skeletal progenitors and the GNAS gene: fibrous dysplasia of bone read through stem cells
Q21184116Violating the splicing rules: TG dinucleotides function as alternative 3' splice sites in U2-dependent introns

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