Protein Digestion for DIGE Analysis

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Protein Digestion for DIGE Analysis is …
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scholarly articleQ13442814

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P356DOI10.1007/978-1-4939-7268-5_17
P698PubMed publication ID29019136

P50authorKay OhlendieckQ59822544
P2093author name stringSandra Murphy
P2860cites workCurrent two-dimensional electrophoresis technology for proteomicsQ22252855
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Difference gel electrophoresis: a single gel method for detecting changes in protein extractsQ28258266
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Improvement of in-gel digestion protocol for peptide mass fingerprinting by matrix-assisted laser desorption/ionization time-of-flight mass spectrometryQ31002939
Peptide mass fingerprintingQ33212134
Comparative study of three proteomic quantitative methods, DIGE, cICAT, and iTRAQ, using 2D gel- or LC-MALDI TOF/TOF.Q33235253
Proteomic analyses using Grifola frondosa metalloendoprotease Lys-N.Q33559849
Value of using multiple proteases for large-scale mass spectrometry-based proteomicsQ33707969
Confetti: a multiprotease map of the HeLa proteome for comprehensive proteomicsQ33718729
Identification of proteins found to be significantly altered when comparing the serum proteome from Multiple Myeloma patients with varying degrees of bone diseaseQ34423112
Overcoming key technological challenges in using mass spectrometry for mapping cell surfaces in tissuesQ34551892
Straightforward ladder sequencing of peptides using a Lys-N metalloendopeptidase.Q34772041
The development of the DIGE system: 2D fluorescence difference gel analysis technologyQ36131099
Enhanced sequence coverage of proteins in human cerebrospinal fluid using multiple enzymatic digestion and linear ion trap LC-MS/MS.Q36505988
Comparative study of workflows optimized for in-gel, in-solution, and on-filter proteolysis in the analysis of plasma membrane proteinsQ39364931
Multiple enzymatic digestion for enhanced sequence coverage of proteins in complex proteomic mixtures using capillary LC with ion trap MS/MS.Q39644537
Six alternative proteases for mass spectrometry-based proteomics beyond trypsin.Q39814223
Two-dimensional fluorescence difference gel electrophoresis for comparative proteomics profilingQ41137577
Prediction of missed cleavage sites in tryptic peptides aids protein identification in proteomicsQ41884528
Informatics and statistics for analyzing 2-d gel electrophoresis imagesQ42078915
Trypsin cleaves exclusively C-terminal to arginine and lysine residuesQ44807551
New protein isoforms identified within Arabidopsis thaliana seed oil bodies combining chymotrypsin/trypsin digestion and peptide fragmentation analysisQ45101067
On-line multi-enzymatic approach for improved sequence coverage in protein analysis.Q46081229
Comparative Label-Free Mass Spectrometric Analysis of Mildly versus Severely Affected mdx Mouse Skeletal Muscles Identifies Annexin, Lamin, and Vimentin as Universal Dystrophic Markers.Q53457776
Large-Scale Quantitative Assessment of Different In-Solution Protein Digestion Protocols Reveals Superior Cleavage Efficiency of Tandem Lys-C/Trypsin Proteolysis over Trypsin DigestionQ57666105
P407language of work or nameEnglishQ1860
P304page(s)223-232
P577publication date2018-01-01
P1433published inMethods in Molecular BiologyQ15752859
P1476titleProtein Digestion for DIGE Analysis
P478volume1664

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Q104617025Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleencites workP2860

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