Digitonin-permeabilization of astrocytes in culture monitored by trypan blue exclusion and loss of S100B by ELISA.

scientific article published in November 2000

Digitonin-permeabilization of astrocytes in culture monitored by trypan blue exclusion and loss of S100B by ELISA. is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1016/S1385-299X(00)00041-6
P698PubMed publication ID11086267

P2093author name stringKarl J
Mendez A
Gonçalves CA
Gottfried C
Tramontina F
Gonçalves D
Portela LV
P2860cites workThe use of permeabilized cells to assay protein phosphorylation and catecholamine releaseQ34004664
A practical guide to the preparation of Ca2+ buffersQ40761762
Cell membrane resealing by a vesicular mechanism similar to neurotransmitter releaseQ42802526
Permeabilizing cells: some methods and applications for the study of intracellular processesQ45026832
Astrocytes possess prostaglandin F2α receptors coupled to phospholipase CQ45099515
Regulation of protein phosphorylation in astrocyte cultures by external calcium ions: specific effects on the phosphorylation of glial fibrillary acidic protein (GFAP), vimentin and heat shock protein 27 (HSP27).Q48182314
Calcium-modulated proteins change their immunoreactivity in the presence of Ca2+: a study of antibody recognition in a dot immunoassay for calmodulin, calcineurin (beta-subunit), and S100B.Q48598938
Impaired Ca-signaling in astrocytes from the Ts16 mouse model of Down syndromeQ48796690
GFAP phosphorylation studied in digitonin-permeabilized astrocytes: standardization of conditions.Q50514801
Simultaneous measurement of tyrosine hydroxylase activity and phosphorylation in bovine adrenal chromaffin cellsQ63435619
Loss and Ca(2+)-dependent retention of scinderin in digitonin-permeabilized chromaffin cells: correlation with Ca(2+)-evoked catecholamine releaseQ67558705
Regulated exocytotic secretion from permeabilized cellsQ67994061
Calcium homeostasis in digitonin-permeabilized bovine chromaffin cellsQ69919243
A specific and sensitive ELISA for measuring S-100b in cerebrospinal fluidQ73543859
P433issue1-2
P921main subjectastrocyteQ502961
P304page(s)86-90
P577publication date2000-11-01
P1433published inBrain Research ProtocolsQ16039923
P1476titleDigitonin-permeabilization of astrocytes in culture monitored by trypan blue exclusion and loss of S100B by ELISA.
P478volume6

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cites work (P2860)
Q42528512Developmental changes in S100B content in brain tissue, cerebrospinal fluid, and astrocyte cultures of rats
Q46821646Effects of early-life LiCl-pilocarpine-induced status epilepticus on memory and anxiety in adult rats are associated with mossy fiber sprouting and elevated CSF S100B protein
Q47161864Encapsulation of Piper cabralanum (Piperaceae) nonpolar extract in poly(methyl methacrylate) by miniemulsion and evaluation of increase in the effectiveness of antileukemic activity in K562 cells
Q40037989Epicatechin gallate increases glutamate uptake and S100B secretion in C6 cell lineage
Q42526281Glutamate uptake in cultured astrocytes depends on age: a study about the effect of guanosine and the sensitivity to oxidative stress induced by H(2)O(2).
Q48570923Glutamate uptake is stimulated by extracellular S100B in hippocampal astrocytes
Q48489255High glutamate decreases S100B secretion by a mechanism dependent on the glutamate transporter
Q64098095Nuclear import of Xenopus egg extract components into cultured cells for reprogramming purposes: a case study on goldfish fin cells
Q48124985Protective effects of resveratrol on hydrogen peroxide induced toxicity in primary cortical astrocyte cultures
Q48143955Resveratrol increases glutamate uptake, glutathione content, and S100B secretion in cortical astrocyte cultures.
Q46955122Transitory gliosis in the CA3 hippocampal region in rats fed on a ketogenic diet.

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