Using Disruptive Insertional Mutagenesis to Identify the In Situ Structure-Function Landscape of the Shigella Translocator Protein IpaB. (scientific article published on 19 April 2018)

IpaD of Shigella flexneri is independently required for regulation of Ipa protein secretion and efficient insertion of IpaB and IpaC into host membranes.

Q40454544

Oligomeric states of the Shigella translocator protein IpaB provide structural insights into formation of the type III secretion translocon.

Q40564027

IpaC from Shigella and SipC from Salmonella possess similar biochemical properties but are functionally distinct

Q41474021

Extracellular association and cytoplasmic partitioning of the IpaB and IpaC invasins of S. flexneri

Q41497452

N-terminus of IpaB provides a potential anchor to the Shigella type III secretion system tip complex protein IpaD.

Q41785011

IpaD localizes to the tip of the type III secretion system needle of Shigella flexneri

Q41856079

Binding affects the tertiary and quaternary structures of the Shigella translocator protein IpaB and its chaperone IpgC.

Q41893547

Liposomes recruit IpaC to the Shigella flexneri type III secretion apparatus needle as a final step in secretion induction.

Q42128361

Three-dimensional electron microscopy reconstruction and cysteine-mediated crosslinking provide a model of the type III secretion system needle tip complex.

Q42566246

Genetic Dissection of the Signaling Cascade that Controls Activation of the Shigella Type III Secretion System from the Needle Tip.

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Bile salts stimulate recruitment of IpaB to the Shigella flexneri surface, where it colocalizes with IpaD at the tip of the type III secretion needle

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An optimized system for expression and purification of secreted bacterial proteins

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Inoculum size in shigellosis and implications for expected mode of transmission

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Recombinant Expression and Purification of the Shigella Translocator IpaB.

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Characterization of Type Three Secretion System Translocator Interactions with Phospholipid Membranes.

Q51330899

Preparation and characterization of translocator/chaperone complexes and their component proteins from Shigella flexneri.

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The type III secretion system needle tip complex mediates host cell sensing and translocon insertion.

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XtalPred: a web server for prediction of protein crystallizability

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High-throughput imaging of bacterial colonies grown on filter plates with application to serum bactericidal assays

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Biochemistry. Resolving some old problems in protein crystallography

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CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice

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PHENIX: a comprehensive Python-based system for macromolecular structure solution

Q24654617

Cellular Aspects of Shigella Pathogenesis: Focus on the Manipulation of Host Cell Processes

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The Many Faces of IpaB

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IpaB-IpgC interaction defines binding motif for type III secretion translocator

Q27655665

Combination of Two Separate Binding Domains Defines Stoichiometry between Type III Secretion System Chaperone IpgC and Translocator Protein IpaB

Q27664968

The Structures of Coiled-Coil Domains from Type III Secretion System Translocators Reveal Homology to Pore-Forming Toxins

Q27677178

Structure of bacteriophage T4 lysozyme refined at 1.7 A resolution

Q27728620

XDS

Q27860472

Coot: model-building tools for molecular graphics

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ESPript: analysis of multiple sequence alignments in PostScript

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Optimal description of a protein structure in terms of multiple groups undergoing TLS motion

Q27860825

Linking Crystallographic Model and Data Quality

Q27860887

Improved R-factors for diffraction data analysis in macromolecular crystallography

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PHENIX: building new software for automated crystallographic structure determination

Q27861044

Features and development of Coot

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Scaling and assessment of data quality

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An introduction to data reduction: space-group determination, scaling and intensity statistics

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Shigella flexneri effectors OspE1 and OspE2 mediate induced adherence to the colonic epithelium following bile salts exposure

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Characterization of the interaction partners of secreted proteins and chaperones of Shigella flexneri.

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Global burden of Shigella infections: implications for vaccine development and implementation of control strategies

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Structure-function analysis of the Shigella virulence factor IpaB.

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Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study

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Influence of oligomerization state on the structural properties of invasion plasmid antigen B from Shigella flexneri in the presence and absence of phospholipid membranes.

Q34389407

Decreasing Shigellosis-related Deaths withoutShigellaspp.–specific Interventions, Asia

Q35804868

Nonpolar mutagenesis of the ipa genes defines IpaB, IpaC, and IpaD as effectors of Shigella flexneri entry into epithelial cells

Q36122200

Molecular pathogenesis of Shigella spp.: controlling host cell signaling, invasion, and death by type III secretion.

Q36422508

Assembly, structure, function and regulation of type III secretion systems.

Q39230405

Deoxycholate interacts with IpaD of Shigella flexneri in inducing the recruitment of IpaB to the type III secretion apparatus needle tip.

Q39627554

The extreme C terminus of Shigella flexneri IpaB is required for regulation of type III secretion, needle tip composition, and binding.

Q39896038

P433

issue

P304

page(s)

1392-1406

P577

publication date

2018-05-03

P1433

published in

Protein Science

Q7251445

P1476

title

Using disruptive insertional mutagenesis to identify the in situ structure-function landscape of the Shigella translocator protein IpaB

P478

volume

Using Disruptive Insertional Mutagenesis to Identify the In Situ Structure-Function Landscape of the Shigella Translocator Protein IpaB.

scientific article published on 19 April 2018

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