Expression and characterization of human proinsulin fused to thioredoxin in Escherichia coli.

scientific article

Expression and characterization of human proinsulin fused to thioredoxin in Escherichia coli. is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1007/S00253-011-3721-5
P698PubMed publication ID22139017
P5875ResearchGate publication ID51851189

P2093author name stringEdgardo Poskus
Aldana Trabucchi
Luciano Lucas Guerra
Natalia Inés Faccinetti
Ruben Francisco Iacono
Silvina Noemí Valdez
P2860cites workTricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDaQ27861105
Periplasmic production of native human proinsulin as a fusion to E. coli ecotinQ30319826
Increased production of human proinsulin in the periplasmic space of Escherichia coli by fusion to DsbA.Q31613031
Recombinant human insulin. VIII. Isolation of fusion protein--S-sulfonate, biotechnological precursor of human insulin, from the biomass of transformed Escherichia coli cellsQ31854679
Following the leader: bacterial protein export through the Sec pathway.Q33702236
Recombinant protein expression in Escherichia coliQ33744852
Expression and secretion of proteins in E. coli.Q33770572
The net charge of the first 18 residues of the mature sequence affects protein translocation across the cytoplasmic membrane of gram-negative bacteriaQ33789148
Yeast secretory expression of insulin precursorsQ33921303
Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion.Q34685284
Short synthetic oligodeoxyribonucleotide leader sequences enhance accumulation of human proinsulin synthesized in Escherichia coliQ35587703
Getting out: protein traffic in prokaryotesQ35720976
Cellular location affects protein stability in Escherichia coliQ36287012
Biosynthesis and periplasmic segregation of human proinsulin in Escherichia coliQ36366881
Secretion and processing of insulin precursors in yeastQ37398799
Multiple joined genes prevent product degradation in Escherichia coliQ37560053
Mature high-affinity immune responses to (pro)insulin anticipate the autoimmune cascade that leads to type 1 diabetesQ39676482
Expression of properly folded human glutamate decarboxylase 65 as a fusion protein in Escherichia coli.Q40892850
Expression of correctly folded proteins in Escherichia coliQ41099605
Evaluation of bottlenecks in proinsulin secretion by Escherichia coli.Q47822866
An 'internal' signal sequence directs secretion and processing or proinsulin in bacteriaQ48407502
Improved synthesis of Salmonella typhimurium enterotoxin using gene fusion expression systemsQ50148022
Purification by immobilized metal affinity chromatography of human atrial natriuretic peptide expressed in a novel thioredoxin fusion protein.Q54613288
A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm.Q54663005
Bacillusamyloliquefaciens α -amylase signal sequence fused in frame with human proinsulin is properly processed by Bacillussubtilis cellsQ57167819
P433issue6
P407language of work or nameEnglishQ1860
P921main subjectEscherichia coliQ25419
P304page(s)1565-1576
P577publication date2011-12-03
P1433published inApplied Microbiology and BiotechnologyQ13553694
P1476titleExpression and characterization of human proinsulin fused to thioredoxin in Escherichia coli.
P478volume94

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cites work (P2860)
Q61443107Expression of recombinant glutamic acid decarboxylase in insect larvae and its application in an immunoassay for the diagnosis of autoimmune diabetes mellitus
Q53562750Flow cytometric microsphere-based immunoassay as a novel non-radiometric method for the detection of glutamic acid decarboxylase autoantibodies in type 1 diabetes mellitus.
Q36202941Novel prokaryotic expression of thioredoxin-fused insulinoma associated protein tyrosine phosphatase 2 (IA-2), its characterization and immunodiagnostic application
Q46556379Prokaryotic expression and characterization of the heterodimeric construction of ZnT8 and its application for autoantibodies detection in diabetes mellitus
Q35839894Surface plasmon resonance reveals a different pattern of proinsulin autoantibodies concentration and affinity in diabetic patients
Q40165245The 3D protein of duck hepatitis A virus type 1 binds to a viral genomic 3' UTR and shows RNA-dependent RNA polymerase activity.
Q40990880Use of cross-reactivity immunoassay to orient insulin replacement in diabetic patients with high levels of insulin antibodies

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