Engineering of polyhydroxyalkanoate (PHA) synthase PhaC2Ps of Pseudomonas stutzeri via site-specific mutation for efficient production of PHA copolymers.

scientific article published on 21 April 2011

Engineering of polyhydroxyalkanoate (PHA) synthase PhaC2Ps of Pseudomonas stutzeri via site-specific mutation for efficient production of PHA copolymers. is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1007/S00253-011-3274-7
P698PubMed publication ID21509565

P2093author name stringGuo-Qiang Chen
Ge Song
Xiao-Wen Shen
Zhen-Yu Shi
Zheng-Jun Li
P2860cites workFour new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettesQ29615258
Cloning and molecular analysis of the poly(3-hydroxyalkanoic acid) gene locus of Pseudomonas aeruginosa PAO1Q30992893
Enhanced synthesis of poly(3-hydroxybutyrate) in recombinant Escherichia coli by means of error-prone PCR mutagenesis, saturation mutagenesis, and in vitro recombination of the type II polyhydroxyalkanoate synthase geneQ31142999
Altering the substrate specificity of polyhydroxyalkanoate synthase 1 derived from Pseudomonas putida GPo1 by localized semirandom mutagenesisQ33203904
Biochemical and genetic analysis of PHA synthases and other proteins required for PHA synthesis.Q33693473
Enhanced accumulation and changed monomer composition in polyhydroxyalkanoate (PHA) copolyester by in vitro evolution of Aeromonas caviae PHA synthaseQ34114327
The application of polyhydroxyalkanoates as tissue engineering materialsQ34425192
Replacement of the catalytic nucleophile cysteine-296 by serine in class II polyhydroxyalkanoate synthase from Pseudomonas aeruginosa-mediated synthesis of a new polyester: identification of catalytic residuesQ34536317
Evolution of polyhydroxyalkanoate (PHA) production system by "enzyme evolution": successful case studies of directed evolutionQ35910665
PHA synthase engineering toward superbiocatalysts for custom-made biopolymers.Q36661632
Increased diversification of polyhydroxyalkanoates by modification reactions for industrial and medical applicationsQ36674029
Biotechnological approaches for the production of polyhydroxyalkanoates in microorganisms and plants - a reviewQ36707673
A microbial polyhydroxyalkanoates (PHA) based bio- and materials industryQ37558302
13C nuclear magnetic resonance studies of Pseudomonas putida fatty acid metabolic routes involved in poly(3-hydroxyalkanoate) synthesisQ39930994
Cloning of the Alcaligenes eutrophus genes for synthesis of poly-beta-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coliQ39955218
Poly-3-hydroxyalkanoate synthases from Pseudomonas putida U: substrate specificity and ultrastructural studies.Q41876828
Expression of 3-ketoacyl-acyl carrier protein reductase (fabG) genes enhances production of polyhydroxyalkanoate copolymer from glucose in recombinant Escherichia coli JM109Q42122544
In vivo evolution of the Aeromonas punctata polyhydroxyalkanoate (PHA) synthase: isolation and characterization of modified PHA synthases with enhanced activityQ44097857
Studies on comonomer compositional distribution of bacterial poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)s and thermal characteristics of their factionsQ44128829
Metabolic engineering for the production of copolyesters consisting of 3-hydroxybutyrate and 3-hydroxyhexanoate by Aeromonas hydrophilaQ45093145
Production and characterization of homopolymer poly(3-hydroxyvalerate) (PHV) accumulated by wild type and recombinant Aeromonas hydrophila strain 4AK4.Q46036269
Metabolic engineering of Aeromonas hydrophila for the enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate).Q46571164
Biosynthesis and characterization of poly(3-hydroxybutyrate-co-3- hydroxyhexanoate) from palm oil products in a Wautersia eutropha mutant.Q46744766
A lower specificity PhaC2 synthase from Pseudomonas stutzeri catalyses the production of copolyesters consisting of short-chain-length and medium-chain-length 3-hydroxyalkanoatesQ46958844
Alteration of substrate chain-length specificity of type II synthase for polyhydroxyalkanoate biosynthesis by in vitro evolution: in vivo and in vitro enzyme assaysQ47996536
Biosynthesis and properties of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) by recombinant strains of Pseudomonas sp. 61-3.Q52544575
P433issue3
P407language of work or nameEnglishQ1860
P921main subjectpolyhydroxyalkanoateQ425079
Pseudomonas stutzeriQ2888828
P304page(s)655-665
P577publication date2011-04-21
P1433published inApplied Microbiology and BiotechnologyQ13553694
P1476titleEngineering of polyhydroxyalkanoate (PHA) synthase PhaC2Ps of Pseudomonas stutzeri via site-specific mutation for efficient production of PHA copolymers.
P478volume91

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cites work (P2860)
Q47575486Modeling the bioconversion of starch to P(HB-co-HV) optimized by experimental design using Bacillus megaterium BBST4 strain.
Q36416683Production of copolyesters of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates by E. coli containing an optimized PHA synthase gene
Q92942472Role of PhaC Type I and Type II Enzymes during PHA Biosynthesis

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