Mutational analysis of polynucleotide phosphorylase from Escherichia coli.

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Mutational analysis of polynucleotide phosphorylase from Escherichia coli. is …
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scholarly articleQ13442814

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P356DOI10.1016/S0022-2836(02)00645-9
P698PubMed publication ID12162954

P2093author name stringNathalie Mathy
Dominique Bréchemier-Baey
Claude Portier
Ophélie Duché
Anne Jarrige
P2860cites workLocalized Bicaudal-C RNA encodes a protein containing a KH domain, the RNA binding motif of FMR1.Q37696957
Kinetics of Polymerization and Phosphorolysis Reactions of E. coli Polynucleotide Phosphorylase. Role of Oligonucleotides in PolymerizationQ38362986
Increased expression of Escherichia coli polynucleotide phosphorylase at low temperatures is linked to a decrease in the efficiency of autocontrolQ39503894
In vitro genetic analysis of the RNA binding site of vigilin, a multi-KH-domain proteinQ39575228
Guanosine pentaphosphate synthetase from Streptomyces antibioticus is also a polynucleotide phosphorylaseQ39842042
Low copy number plasmids for regulated low-level expression of cloned genes in Escherichia coli with blue/white insert screening capabilityQ40519280
A point mutation in the FMR-1 gene associated with fragile X mental retardationQ41579799
Isolation of a polynucleotide phosphorylase mutant using a kanamycin resistant determinantQ44087137
Kinetics of Polymerization and Phosphorolysis Reactions of Escherichia coli Polynucleotide Phosphorylase. Evidence for Multiple Binding of Polynucleotide in PhosphorolysisQ47767974
Quaternary Structure of Polynucleotide Phosphorylase from Escherichia coli: Evidence of a Complex between Two Types of Polypeptide ChainsQ52872640
Electron microscopy of Escherichia coli polynucleotide phosphorylase molecules and polyribonucleotide formationQ53692775
[Kinetics of reactions catalyzed by Escherichia coli polynucleotide phosphorylase. 2-deoxy-ADP as substrate and inhibitor].Q53790760
Copurification of E. coli RNAase E and PNPase: evidence for a specific association between two enzymes important in RNA processing and degradation.Q54637941
A novel mutation in the KH domain of polynucleotide phosphorylase affects autoregulation and mRNA decay in Escherichia coliQ57732836
Quaternary structure ofEscherichia colipolynucleotide phosphorylase: new evidence for a trimeric structureQ66891610
Essential role for KH domains in RNA binding: impaired RNA binding by a mutation in the KH domain of FMR1 that causes fragile X syndromeQ24310518
Polynucleotide phosphorylase functions as both an exonuclease and a poly(A) polymerase in spinach chloroplastsQ24550883
KH domain: one motif, two foldsQ24625767
A duplicated fold is the structural basis for polynucleotide phosphorylase catalytic activity, processivity, and regulationQ27628410
The solution structure of the S1 RNA binding domain: a member of an ancient nucleic acid-binding foldQ27734665
Rapid and efficient site-specific mutagenesis without phenotypic selectionQ27860608
The protein product of the fragile X gene, FMR1, has characteristics of an RNA-binding proteinQ28117885
The first step in the functional inactivation of the Escherichia coli polynucleotide phosphorylase messenger is a ribonuclease III processing at the 5' end.Q33929386
PNPase autocontrols its expression by degrading a double-stranded structure in the pnp mRNA leaderQ34103527
RNA degradosomes exist in vivo in Escherichia coli as multicomponent complexes associated with the cytoplasmic membrane via the N-terminal region of ribonuclease E.Q34583159
Polynucleotide phosphorylase functions both as a 3' right-arrow 5' exonuclease and a poly(A) polymerase in Escherichia coliQ35358676
Polynucleotide phosphorylase of Escherichia coli induces the degradation of its RNase III processed messenger by preventing its translationQ37585931
P433issue3
P407language of work or nameEnglishQ1860
P921main subjectmutational analysisQ1955810
Escherichia coliQ25419
P304page(s)397-409
P577publication date2002-08-01
P1433published inJournal of Molecular BiologyQ925779
P1476titleMutational analysis of polynucleotide phosphorylase from Escherichia coli.
P478volume321

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cites work (P2860)
Q50165023"A high-throughput and rapid computational method for screening of RNA post-transcriptional modifications that can be recognized by target proteins".
Q40695776A mutation in polynucleotide phosphorylase from Escherichia coli impairing RNA binding and degradosome stability
Q24303834Analysis of the human polynucleotide phosphorylase (PNPase) reveals differences in RNA binding and response to phosphate compared to its bacterial and chloroplast counterparts
Q43173275Autogenous regulation of Escherichia coli polynucleotide phosphorylase expression revisited
Q92976778Bacterial ribonucleases and their roles in RNA metabolism
Q92980877Computational evolution of an RNA-binding protein towards enhanced oxidized-RNA binding
Q35813310Cooperation of endo- and exoribonucleases in chloroplast mRNA turnover
Q27654474Crystal Structure of Escherichia coli Polynucleotide Phosphorylase Core Bound to RNase E, RNA and Manganese: Implications for Catalytic Mechanism and RNA Degradosome Assembly
Q27676507Crystal structure of human polynucleotide phosphorylase: insights into its domain function in RNA binding and degradation
Q27641246Crystal structure of the tRNA processing enzyme RNase PH from Aquifex aeolicus
Q37909123Current perspectives of the Escherichia coli RNA degradosome
Q37653293CvfA protein and polynucleotide phosphorylase act in an opposing manner to regulate Staphylococcus aureus virulence
Q24311687Defining the domains of human polynucleotide phosphorylase (hPNPaseOLD-35) mediating cellular senescence
Q41948055Discrimination of RNA from DNA by polynucleotide phosphorylase
Q41887991Distinctive effects of domain deletions on the manganese-dependent DNA polymerase and DNA phosphorylase activities of Mycobacterium smegmatis polynucleotide phosphorylase
Q24682917Domain analysis of the chloroplast polynucleotide phosphorylase reveals discrete functions in RNA degradation, polyadenylation, and sequence homology with exosome proteins
Q39362009Function of the conserved S1 and KH domains in polynucleotide phosphorylase
Q89985668Functions of Conserved Domains of Human Polynucleotide Phosphorylase on RNA Oxidation
Q47385179Genomic analysis in Escherichia coli demonstrates differential roles for polynucleotide phosphorylase and RNase II in mRNA abundance and decay
Q37114470Human polynucleotide phosphorylase (hPNPase(old-35)): an evolutionary conserved gene with an expanding repertoire of RNA degradation functions
Q33635838Inhibition of homologous phosphorolytic ribonucleases by citrate may represent an evolutionarily conserved communicative link between RNA degradation and central metabolism
Q36727333Interaction of Bacillus subtilis Polynucleotide Phosphorylase and RNase Y: STRUCTURAL MAPPING AND EFFECT ON mRNA TURNOVER.
Q41460882Modulation of yersinia type three secretion system by the S1 domain of polynucleotide phosphorylase
Q54598720Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3'-end maturation and intron degradation.
Q35705486PNPASE and RNA trafficking into mitochondria
Q90341372PNPase knockout results in mtDNA loss and an altered metabolic gene expression program
Q36637903Polynucleotide Phosphorylase Regulates Multiple Virulence Factors and the Stabilities of Small RNAs RsmY/Z in Pseudomonas aeruginosa
Q39888660Polynucleotide phosphorylase exonuclease and polymerase activities on single-stranded DNA ends are modulated by RecN, SsbA and RecA proteins
Q42075370Polynucleotide phosphorylase hinders mRNA degradation upon ribosomal protein S1 overexpression in Escherichia coli
Q27642395Probing the Functional Importance of the Hexameric Ring Structure of RNase PH
Q37392521RNA polyadenylation and decay in mitochondria and chloroplasts
Q39794684RNase E-based degradosome modulates polyadenylation of mRNAs after Rho-independent transcription terminators in Escherichia coli
Q40310390RNase III-Independent Autogenous Regulation of Escherichia coli Polynucleotide Phosphorylase via Translational Repression
Q33349686RNase activity of polynucleotide phosphorylase is critical at low temperature in Escherichia coli and is complemented by RNase II
Q26770015Regulation and functions of bacterial PNPase
Q35069589Ribonucleases and bacterial virulence
Q34985832Role of polynucleotide phosphorylase in sRNA function in Escherichia coli
Q39799322S1 and KH domains of polynucleotide phosphorylase determine the efficiency of RNA binding and autoregulation
Q42930083Streptomyces coelicolor polynucleotide phosphorylase can polymerize nucleoside diphosphates under phosphorolysis conditions, with implications for the degradation of structured RNAs
Q35112323Structural components and architectures of RNA exosomes
Q36444236The Protein Interaction of RNA Helicase B (RhlB) and Polynucleotide Phosphorylase (PNPase) Contributes to the Homeostatic Control of Cysteine in Escherichia coli
Q28256090The archaeal exosome core is a hexameric ring structure with three catalytic subunits
Q37775698The critical role of RNA processing and degradation in the control of gene expression.
Q37392505The role of 3'-5' exoribonucleases in RNA degradation
Q42952287The role of the S1 domain in exoribonucleolytic activity: substrate specificity and multimerization
Q42552263Thioflavin T as a fluorescence probe for monitoring RNA metabolism at molecular and cellular levels