5 Studying Essential Genes: Generating and Using Promoter Fusions and Conditional Alleles

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5 Studying Essential Genes: Generating and Using Promoter Fusions and Conditional Alleles is …
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scholarly articleQ13442814

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P356DOI10.1016/S0580-9517(06)36005-9

P2093author name stringMichael JR Stark
P2860cites workUninterrupted MCM2-7 function required for DNA replication fork progressionQ27929772
Budding yeast SKP1 encodes an evolutionarily conserved kinetochore protein required for cell cycle progressionQ27931347
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New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sitesQ28131597
A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettesQ28131622
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Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expressionQ28131772
Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiaeQ29546523
Sequences That Regulate the Divergent GAL1-GAL10 Promoter in Saccharomyces cerevisiaeQ29547797
In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant yeastQ29618550
Evidence that the Ipl1-Sli15 (Aurora kinase-INCENP) complex promotes chromosome bi-orientation by altering kinetochore-spindle pole connectionsQ29619579
A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiaeQ29620316
Use of polymerase chain reaction epitope tagging for protein tagging in Saccharomyces cerevisiaeQ29620841
Box C/D small nucleolar RNA trafficking involves small nucleolar RNP proteins, nucleolar factors and a novel nuclear domainQ34077876
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Closing the cell cycle circle in yeast: G2 cyclin proteolysis initiated at mitosis persists until the activation of G1 cyclins in the next cycle.Q34329439
Functions and functional domains of the GTPase Cdc42pQ34674425
An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeastQ39723005
The Saccharomyces cerevisiae gene SDS22 encodes a potential regulator of the mitotic function of yeast type 1 protein phosphataseQ40016672
The new yeast geneticsQ40158613
The Cdc31p-binding protein Kar1p is a component of the half bridge of the yeast spindle pole bodyQ42771276
The degradation signal in a short-lived proteinQ45180905
Four major transcriptional responses in the methionine/threonine biosynthetic pathway of Saccharomyces cerevisiae.Q45287576
Heat-inducible degron: a method for constructing temperature-sensitive mutants.Q45930863
Doxycycline, the drug used to control the tet-regulatable promoter system, has no effect on global gene expression in Saccharomyces cerevisiaeQ46534208
Rapid depletion of budding yeast proteins by fusion to a heat-inducible degronQ47976542
The relative merits of the tetO2 and tetO7 promoter systems for the functional analysis of heterologous genes in yeast and a compilation of essential yeast genes with tetO2 promoter substitutions.Q49169000
A regulatedMET3-GLC7 gene fusion provides evidence of a mitotic role forSaccharomyces cerevisiae protein phosphatase 1Q58197792
P304page(s)79-102
P577publication date2007-01-01
P1433published inMethods in MicrobiologyQ15767395
P1476title5 Studying Essential Genes: Generating and Using Promoter Fusions and Conditional Alleles

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