Is Research on “Synthetic Cells” Moving to the Next Level?

scientific article published on 26 December 2018

Is Research on “Synthetic Cells” Moving to the Next Level? is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.3390/LIFE9010003
P932PMC publication ID6463193
P698PubMed publication ID30587790

P50authorPasquale StanoQ59413847
P2093author name stringPasquale Stano
P2860cites workNon-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesisQ21131315
Towards synthesis of a minimal cellQ24672349
Constructive Approaches for Understanding the Origin of Self-Replication and EvolutionQ26740084
Octanol-assisted liposome assembly on chipQ27334711
Design and synthesis of a minimal bacterial genomeQ28005478
Artificial cells: prospects for biotechnologyQ28200982
Synthesizing lifeQ28201137
Approaches to semi-synthetic minimal cells: a reviewQ28282252
Creation of a bacterial cell controlled by a chemically synthesized genomeQ28283016
DNA-mediated self-assembly of artificial vesiclesQ28473431
Hierarchical unilamellar vesicles of controlled compositional heterogeneityQ28485251
RNA catalysis in model protocell vesiclesQ28821481
Organisms ≠ MachinesQ30432572
Crude phosphorylation mixtures containing racemic lipid amphiphiles self-assemble to give stable primitive compartments.Q47134655
A modular approach for multifunctional polymersomes with controlled adhesive propertiesQ47199164
Synthetic Cells Synthesize Therapeutic Proteins inside Tumors.Q47214708
Integrated Microfluidic System for Size-Based Selection and Trapping of Giant VesiclesQ47338353
Towards an in vivo biologically inspired nanofactoryQ47432729
An integrated self-assembled nanofluidic system for controlled biological chemistriesQ47585843
Organization of chemical reactions into dividing and metabolizing units: The chemotonsQ47679647
Protein expression in liposomesQ47709579
Experiment and mathematical modeling of gene expression dynamics in a cell-free systemQ47937082
Cogenerating Synthetic Parts toward a Self-Replicating SystemQ48044180
Gene-Mediated Chemical Communication in Synthetic Protocell CommunitiesQ48089013
Microfluidic Assembly of Monodisperse Vesosomes as Artificial Cell ModelsQ48149597
Synthetic cells produce a quorum sensing chemical signal perceived by Pseudomonas aeruginosaQ48244451
In Vitro Reconstitution and Optimization of the Entire Pathway to Convert Glucose into Fatty AcidQ48358865
Tailoring the appearance: what will synthetic cells look like?Q49572520
Do protocells preferentially retain macromolecular solutes upon division/fragmentation? A study based on the extrusion of POPC giant vesicles.Q49915521
Gene expression within cell-sized lipid vesiclesQ79287861
A relationship between membrane properties forms the basis of a selectivity mechanism for vesicle self-reproductionQ79955477
Population analysis of structural properties of giant liposomes by flow cytometryQ84385894
Oriented reconstitution of a membrane protein in a giant unilamellar vesicle: experimental verification with the potassium channel KcsAQ84439231
ENVIRONMENT: a computational platform to stochastically simulate reacting and self-reproducing lipid compartmentsQ84724550
Stochasticity in gene expression in a cell-sized compartmentQ85674095
Photosynthetic artificial organelles sustain and control ATP-dependent reactions in a protocellular systemQ88881608
Robustness of a Reconstituted Escherichia coli Protein Translation System Analyzed by Computational ModelingQ90030076
Droplet microfluidics for the construction of compartmentalised model membranesQ90677703
The Potential for Convergence between Synthetic Biology and BioelectronicsQ91650996
The lipid bilayer membrane and its protein constituentsQ91734502
On protein crowding and bilayer bulging in spontaneous vesicle formationQ95422738
Sustained-release morphine for epidural analgesia in rats.Q46057891
Encapsulation of bilayer vesicles by self-assemblyQ46185456
A synthetic biology approach to the construction of membrane proteins in semi-synthetic minimal cells.Q46232392
Growing Membranes In Vitro by Continuous Phospholipid Biosynthesis from Free Fatty Acids.Q46304922
Synthetic microbial consortia enable rapid assembly of pure translation machinery.Q46623597
A Simple Protein Synthesis Model for the PURE System Operation.Q46737747
Synthesis of functional protein in liposomeQ46759592
Using giant unilamellar lipid vesicle micro-patterns as ultrasmall reaction containers to observe reversible ATP synthesis/hydrolysis of F0F1-ATPase directlyQ46969241
In vitro gene expression within membrane-free coacervate protocells.Q50442552
Defined DNA-mediated assemblies of gene-expressing giant unilamellar vesicles.Q50467717
Protein expression, aggregation, and triggered release from polymersomes as artificial cell-like structures.Q50510058
Protein synthesis in liposomes with a minimal set of enzymes.Q50665797
Quantification of structural properties of cell-sized individual liposomes by flow cytometry.Q50711439
Femtoliter compartment in liposomes for in vitro selection of proteins.Q50720328
First moves towards photoautotrophic synthetic cells: In vitro study of photosynthetic reaction centre and cytochrome bc1 complex interactions.Q50901705
An E. coli cell-free expression toolbox: application to synthetic gene circuits and artificial cells.Q51014018
Using a Redox Modality to Connect Synthetic Biology to Electronics: Hydrogel-Based Chemo-Electro Signal Transduction for Molecular Communication.Q51097647
On-chip microfluidic production of cell-sized liposomes.Q51415377
Expression of a cascading genetic network within liposomes.Q51595632
Coassembly of Photosystem II and ATPase as Artificial Chloroplast for Light-Driven ATP Synthesis.Q51626138
Question 7: new aspects of interactions among vesicles.Q51705999
Autopoietic and (M,R) systemsQ52017319
Mastering Complexity: Towards Bottom-up Construction of Multifunctional Eukaryotic Synthetic Cells.Q52312197
De Novo Synthesis of Basal Bacterial Cell Division Proteins FtsZ, FtsA, and ZipA Inside Giant Vesicles.Q52728482
Co-variation of tRNA abundance and codon usage in Escherichia coli at different growth rates.Q52888301
Polyamine/Nucleotide Coacervates Provide Strong Compartmentalization of Mg²⁺, Nucleotides, and RNA.Q53173152
Self-replication of DNA by its encoded proteins in liposome-based synthetic cells.Q54231286
Functional analysis of membranous Fo-a subunit of F1Fo-ATP synthase by in vitro protein synthesis.Q54346375
Coarse-grained dynamics of protein synthesis in a cell-free system.Q54365364
Protein synthesis by pure translation systems.Q54481387
Machine metaphors and ethics in synthetic biology.Q55065668
Chloroplast-containing coacervate micro-droplets as a step towards photosynthetically active membrane-free protocells.Q55106498
Reverse Transcription Polymerase Chain Reaction in Giant Unilamellar Vesicles.Q55462189
MaxSynBio: Avenues Towards Creating Cells from the Bottom UpQ57180365
The imitation game—a computational chemical approach to recognizing lifeQ57383455
An synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway designQ57825426
Production of RNA by a polymerase protein encapsulated within phospholipid vesiclesQ58001497
Cell-free transcription-translation: engineering biology from the nanometer to the millimeter scaleQ58615997
Liposomes and polymersomes: a comparative review towards cell mimickingQ59368150
Systems chemistryQ59413747
Partitioning Variability of a Compartmentalized In Vitro Transcriptional Thresholding CircuitQ59558011
Plural Embodiment(s) of Mind. Genealogy and Guidelines for a Radically Embodied Approach to Mind and ConsciousnessQ59800620
Communication and quorum sensing in non-living mimics of eukaryotic cellsQ59806187
Minimizing Context Dependency of Gene Networks Using Artificial CellsQ60548756
Gene-Expressing Liposomes as Synthetic Cells for Molecular Communication StudiesQ61814252
Giant Vesicles “Colonies”: A Model for Primitive Cell CommunitiesQ62629904
Achievements and open questions in the self-reproduction of vesicles and synthetic minimal cellsQ62629924
Spontaneous Protein Crowding in Liposomes: A New Vista for the Origin of Cellular MetabolismQ62629936
Charge-controlled microfluidic formation of lipid-based single- and multicompartment systemsQ63446602
Enzymatic RNA replication in self-reproducing vesicles: an approach to a minimal cellQ71673859
Autopoiesis: a review and a reappraisalQ73010497
Polymerase chain reaction in liposomesQ73909530
Triggerable liposomal fusion by enzyme cleavage of a novel peptide-lipid conjugateQ74746687
Reconstitution of membrane proteins into liposomesQ79281001
How many genes can make a cell: the minimal-gene-set conceptQ31029180
A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-basedQ33355845
A vesicle bioreactor as a step toward an artificial cell assemblyQ33582123
Competition between model protocells driven by an encapsulated catalystQ33696471
Designs for life: protocell models in the laboratory.Q34219780
Stochastic simulations of minimal cells: the Ribocell model.Q34247938
Characterization of the emergent properties of a synthetic quasi-cellular system.Q34248087
Comparative properties and methods of preparation of lipid vesicles (liposomes).Q34275078
The calculation of information and organismal complexityQ34279504
Biomimetic liposome- and polymersome-based multicompartmentalized assembliesQ34290199
The emergence of competition between model protocellsQ34346506
Autopoiesis with or without cognition: defining life at its edgeQ34548690
Autopoiesis and cognitionQ34549178
Autopoiesis: the organization of living systems, its characterization and a model.Q34698869
Physical effects underlying the transition from primitive to modern cell membranesQ34750165
Quasi-cellular systems: stochastic simulation analysis at nanoscale rangeQ34788012
Toward the engineering of minimal living cellsQ34968920
Reconstructing the emergence of cellular life through the synthesis of model protocells.Q35001425
Liposome display for in vitro selection and evolution of membrane proteins.Q35182231
Sensing-applications of surface-based single vesicle arraysQ35593723
The vesosome-- a multicompartment drug delivery vehicleQ35644398
A renewal model for the emergence of anomalous solute crowding in liposomesQ35655698
Thirty years of computational autopoiesis: a reviewQ35831900
Determination of the core of a minimal bacterial gene setQ35880926
Engineering asymmetric vesiclesQ35977706
A recursive vesicle-based model protocell with a primitive model cell cycleQ36138480
Cell-Free Phospholipid Biosynthesis by Gene-Encoded Enzymes Reconstituted in LiposomesQ36155383
Towards self-assembled hybrid artificial cells: novel bottom-up approaches to functional synthetic membranesQ36199094
Construction of an in Vitro Gene Screening System of the E. coli EmrE Transporter Using Liposome DisplayQ36278913
Synthetic biology projects in vitroQ36675907
Chemical aspects of synthetic biologyQ36795464
Detergent-mediated incorporation of transmembrane proteins in giant unilamellar vesicles with controlled physiological contentsQ36820230
A simple physical mechanism enables homeostasis in primitive cellsQ37058222
Coupled Growth and Division of Model Protocell MembranesQ37160273
Bacteria as computers making computersQ37328409
Reaction dynamics analysis of a reconstituted Escherichia coli protein translation system by computational modeling.Q37682293
Giant vesicles: preparations and applications.Q37718731
Constructing partial models of cells.Q37762071
Interaction of vesicles: adhesion, fusion and multicompartment systems formationQ37845290
Minimal cells: relevance and interplay of physical and biochemical factors.Q37885887
Giant vesicles as cell modelsQ38029268
Microfluidic methods for forming liposomesQ38071877
The use of liposomes for constructing cell modelsQ38076405
Evolving protocells to prototissues: rational design of a missing linkQ38141058
Progress toward synthetic cellsQ38194195
Membrane protein synthesis in cell-free systems: from bio-mimetic systems to bio-membranesQ38220437
Synthetic cellularity based on non-lipid micro-compartments and protocell models.Q38222139
Toward the assembly of a minimal divisomeQ38241198
Microstructured liposome arrayQ38316729
In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translationQ38461761
Artificial cell mimics as simplified models for the study of cell biologyQ38674809
Two-Way Chemical Communication between Artificial and Natural CellsQ38747821
Membrane protein reconstitution into giant unilamellar vesicles: a review on current techniquesQ38903125
DNA amplification in neutral liposomes for safe and efficient gene deliveryQ39002094
Semi-synthetic minimal cells as a tool for biochemical ICT.Q39662066
Direct formation of proteo-liposomes by in vitro synthesis and cellular cytosolic delivery with connexin-expressing liposomes.Q39855332
Light-activated communication in synthetic tissuesQ39857856
Synthetic organisms and living machines : Positioning the products of synthetic biology at the borderline between living and non-living matterQ39940229
Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomesQ40093485
Integrating artificial with natural cells to translate chemical messages that direct E. coli behaviourQ40469827
In vitro membrane protein synthesis inside Sec translocon-reconstituted cell-sized liposomes.Q41835944
Piecing Together Cell-like SystemsQ41851361
Giant vesicles functionally expressing membrane receptors for an insect pheromoneQ42004811
A mechanical Turing machine: blueprint for a biomolecular computerQ42181018
Engineering genetic circuit interactions within and between synthetic minimal cellsQ42290368
Highly oriented photosynthetic reaction centers generate a proton gradient in synthetic protocellsQ42291312
Synthetic biology of minimal living cells: primitive cell models and semi-synthetic cellsQ42409831
Sugar synthesis in a protocellular model leads to a cell signalling response in bacteriaQ42747290
In vitro membrane protein synthesis inside cell-sized vesicles reveals the dependence of membrane protein integration on vesicle volume.Q43483976
Cell-free translation reconstituted with purified componentsQ43692142
Gene circuit performance characterization and resource usage in a cell-free "breadboard".Q44092139
A simple and versatile method for the formation of arrays of giant vesicles with controlled size and compositionQ44465464
Controlled vesicle self-assembly in microfluidic channels with hydrodynamic focusingQ44783153
Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription-translation system.Q45764642
The minimal size of liposome-based model cells brings about a remarkably enhanced entrapment and protein synthesis.Q45772369
Artificial organelle: ATP synthesis from cellular mimetic polymersomesQ46047340
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue1
P304page(s)3
P577publication date2018-12-26
P1433published inLifeQ17064586
P1476titleIs Research on "Synthetic Cells" Moving to the Next Level?
P478volume9

Reverse relations

cites work (P2860)
Q92636031Investigating Prebiotic Protocells for A Comprehensive Understanding of the Origins of Life: A Prebiotic Systems Chemistry Perspective
Q92290391Modelling Bacteria-Inspired Dynamics with Networks of Interacting Chemicals
Q89790947Primary cell wall inspired micro containers as a step towards a synthetic plant cell

Search more.