Architecture, Function, and Substrates of the Type II Secretion System

scientific article published on 01 February 2019

Architecture, Function, and Substrates of the Type II Secretion System is …
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scholarly articleQ13442814

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P356DOI10.1128/ECOSALPLUS.ESP-0034-2018
P932PMC publication ID6638579
P698PubMed publication ID30767847

P50authorKonstantin V. KorotkovQ57001621
Maria SandkvistQ92503711
P2860cites workThe StcE metalloprotease of enterohaemorrhagic Escherichia coli reduces the inner mucus layer and promotes adherence to human colonic epithelium ex vivo.Q38774649
Identification of protein secretion systems in bacterial genomesQ39026658
Deciphering the Xcp Pseudomonas aeruginosa type II secretion machinery through multiple interactions with substrates.Q39241148
Bacterial surface association of heat-labile enterotoxin through lipopolysaccharide after secretion via the general secretory pathwayQ39455971
Direct interaction of the EpsL and EpsM proteins of the general secretion apparatus in Vibrio choleraeQ39495752
Influence of deletions within domain II of exotoxin A on its extracellular secretion from Pseudomonas aeruginosaQ39500274
Multiple interactions between pullulanase secreton components involved in stabilization and cytoplasmic membrane association of PulE.Q39587073
Legionella pneumophila contains a type II general secretion pathway required for growth in amoebae as well as for secretion of the Msp proteaseQ39613138
TagA is a secreted protease of Vibrio cholerae that specifically cleaves mucin glycoproteinsQ39641288
Identification of XcpZ domains required for assembly of the secreton of Pseudomonas aeruginosaQ39678505
Mutagenesis and isolation of Aeromonas hydrophila genes which are required for extracellular secretionQ39939077
Analysis of eight out genes in a cluster required for pectic enzyme secretion by Erwinia chrysanthemi: sequence comparison with secretion genes from other gram-negative bacteriaQ39939761
Extracellular secretion of pectate lyase by the Erwinia chrysanthemi out pathway is dependent upon Sec-mediated export across the inner membraneQ39942291
Transient entry of enterotoxin subunits into the periplasm occurs during their secretion from Vibrio choleraeQ39956011
Structural dynamics of RbmA governs plasticity of Vibrio cholerae biofilms.Q40111483
Type II Secretion Substrates of Legionella pneumophila Translocate Out of the Pathogen-Occupied Vacuole via a Semipermeable MembraneQ40164466
Alterations in Peptidoglycan Cross-Linking Suppress the Secretin Assembly Defect Caused by Mutation of GspA in the Type II Secretion System.Q40352641
DsbA plays a critical and multifaceted role in the production of secreted virulence factors by the phytopathogen Erwinia carotovora subsp. atroseptica.Q40452562
Txc, a new type II secretion system of Pseudomonas aeruginosa strain PA7, is regulated by the TtsS/TtsR two-component system and directs specific secretion of the CbpE chitin-binding protein.Q40599819
Pseudopilin residue E5 is essential for recruitment by the type 2 secretion system assembly platformQ40645960
Pilus formation and protein secretion by the same machinery in Escherichia coliQ40749053
Interaction between the autokinase EpsE and EpsL in the cytoplasmic membrane is required for extracellular secretion in Vibrio cholerae.Q40806384
Structural insights into the secretin PulD and its trypsin-resistant coreQ41626918
The XcpV/GspI pseudopilin has a central role in the assembly of a quaternary complex within the T2SS pseudopilus.Q41890924
Cysteine scanning mutagenesis and disulfide mapping analysis of arrangement of GspC and GspD protomers within the type 2 secretion systemQ42043696
Assembly of the Type II Secretion System: Identification of ExeA Residues Critical for Peptidoglycan Binding and Secretin MultimerizationQ42120531
Identification of a protein secretory pathway for the secretion of heat-labile enterotoxin by an enterotoxigenic strain of Escherichia coliQ24531356
Structural insights into RbmA, a biofilm scaffolding protein of V. choleraeQ27300114
Modulation of neutrophil function by a secreted mucinase of Escherichia coli O157:H7Q27317705
Type II protein secretion in gram-negative pathogenic bacteria: the study of the structure/secretion relationships of the cellulase Cel5 (formerly EGZ) from Erwinia chrysanthemiQ27634051
Refined crystallographic structure of Pseudomonas aeruginosa exotoxin A and its implications for the molecular mechanism of toxicityQ27636598
Crystal Structure of the Extracellular Protein Secretion NTPase EpsE of Vibrio choleraeQ27642349
Structure of the Minor Pseudopilin EpsH from the Type 2 Secretion System of Vibrio choleraeQ27649752
Structure of the GspK-GspI-GspJ complex from the enterotoxigenic Escherichia coli type 2 secretion systemQ27650459
Crystal Structure of the N-Terminal Domain of the Secretin GspD from ETEC Determined with the Assistance of a NanobodyQ27653797
The three-dimensional structure of the cytoplasmic domains of EpsF from the type 2 secretion system of Vibrio choleraeQ27654465
Calcium Is Essential for the Major Pseudopilin in the Type 2 Secretion SystemQ27656748
The dimer formed by the periplasmic domain of EpsL from the Type 2 Secretion System of Vibrio parahaemolyticusQ27656812
Structural and Functional Studies on the Interaction of GspC and GspD in the Type II Secretion SystemQ27674304
Assembly of the Type II Secretion System such as Found in Vibrio cholerae Depends on the Novel Pilotin AspSQ27675923
Insights into FlaI Functions in Archaeal Motor Assembly and Motility from Structures, Conformations, and GeneticsQ27676405
Structural Basis for Biofilm Formation via the Vibrio cholerae Matrix Protein RbmAQ27678190
Structural insight into the bacterial mucinase StcE essential to adhesion and immune evasion during enterohemorrhagic E. coli infectionQ27678360
A dodecameric ring-like structure of the N0 domain of the type II secretin from enterotoxigenic Escherichia coliQ27678885
Hexamers of the Type II Secretion ATPase GspE from Vibrio cholerae with Increased ATPase ActivityQ27679707
Functional and Structural Characterization of Vibrio cholerae Extracellular Serine Protease B, VesBQ27681443
Crystal structure of the full-length ATPase GspE from the Vibrio vulnificus type II secretion system in complex with the cytoplasmic domain of GspLQ27684938
Structural Basis of Pullulanase Membrane Binding and Secretion Revealed by X-Ray Crystallography, Molecular Dynamics and Biochemical AnalysisQ27703207
Crystal Structure of a Type IV Pilus Assembly ATPase: Insights into the Molecular Mechanism of PilB from Thermus thermophilusQ27728112
Protein motifs. 3. The parallel beta helix and other coiled foldsQ27730429
Minor pseudopilin self-assembly primes type II secretion pseudopilus elongation.Q27939366
Biofilm Matrix ProteinsQ28085056
Involvement of the twin-arginine translocation system in protein secretion via the type II pathwayQ28365509
Crystal structures of an intrinsically active cholera toxin mutant yield insight into the toxin activation mechanismQ28485713
A novel type II secretion system in Pseudomonas aeruginosaQ28492615
Cleavage, methylation, and localization of the Pseudomonas aeruginosa export proteins XcpT, -U, -V, and -WQ28492647
LipC, a second lipase of Pseudomonas aeruginosa, is LipB and Xcp dependent and is transcriptionally regulated by pilus biogenesis componentsQ28492656
Type II protein secretion by Pseudomonas aeruginosa: genetic suppression of a conditional mutation in the pilin-like component XcpT by the cytoplasmic component XcpRQ28492755
Pseudomonas aeruginosa produces an extracellular deoxyribonuclease that is required for utilization of DNA as a nutrient sourceQ28492763
Identification of genes involved in swarming motility using a Pseudomonas aeruginosa PAO1 mini-Tn5-lux mutant libraryQ28492895
Medically Relevant Acinetobacter Species Require a Type II Secretion System and Specific Membrane-Associated Chaperones for the Export of Multiple Substrates and Full VirulenceQ28552441
Zinc coordination is essential for the function and activity of the type II secretion ATPase EpsEQ28822152
Structural insights into the secretin translocation channel in the type II secretion systemQ30152677
Prepore Stability Controls Productive Folding of the BAM-independent Multimeric Outer Membrane Secretin PulD.Q30152695
Assembly of the secretion pores GspD, Wza and CsgG into bacterial outer membranes does not require the Omp85 proteins BamA or TamA.Q30152931
YaeT-independent multimerization and outer membrane association of secretin PulD.Q30157956
Distinct docking and stabilization steps of the Pseudopilus conformational transition path suggest rotational assembly of type IV pilus-like fibersQ30405338
Reassessing the role of the secreted protease CPAF in Chlamydia trachomatis infection through genetic approachesQ30405911
Legionella pneumophila type II secretion dampens the cytokine response of infected macrophages and epitheliaQ30429958
Virulence determinants in the obligate intracellular pathogen Chlamydia trachomatis revealed by forward genetic approachesQ30465552
Secretin PulD: association with pilot PulS, structure, and ion-conducting channel formationQ30727915
The molecular mechanism of the type IVa pilus motorsQ30848174
Proteins secreted via the type II secretion system: smart strategies of Vibrio cholerae to maintain fitness in different ecological nichesQ31111599
The type IV leader peptidase/N-methyltransferase of Vibrio vulnificus controls factors required for adherence to HEp-2 cells and virulence in iron-overloaded miceQ31968540
Molecular analysis of the Vibrio cholerae type II secretion ATPase EpsEQ33553183
Coordinated assembly of multisubunit proteins: oligomerization of bacterial enterotoxins in vivo and in vitroQ33650535
Bacterial type II protein export and pilus biogenesis: more than just homologies?Q33730388
Two regions of EpsL involved in species-specific protein-protein interactions with EpsE and EpsM of the general secretion pathway in Vibrio choleraeQ33993698
Association of the cytoplasmic membrane protein XpsN with the outer membrane protein XpsD in the type II protein secretion apparatus of Xanthomonas campestris pv. campestrisQ33994020
Legionella pneumophila major acid phosphatase and its role in intracellular infectionQ34005553
Type II protein secretion is a subset of the PilD-dependent processes that facilitate intracellular infection by Legionella pneumophilaQ34006686
GlyGly-CTERM and rhombosortase: a C-terminal protein processing signal in a many-to-one pairing with a rhomboid family intramembrane serine proteaseQ34110477
Structural and functional insights into the pilotin-secretin complex of the type II secretion systemQ34162292
Structure of the cholera toxin secretion channel in its closed stateQ34180072
Dissimilatory Fe(III) and Mn(IV) reduction by Shewanella putrefaciens requires ferE, a homolog of the pulE (gspE) type II protein secretion geneQ34304011
Conformation of protein secreted across bacterial outer membranes: a study of enterotoxin translocation from Vibrio choleraeQ34357011
A colonization factor links Vibrio cholerae environmental survival and human infectionQ34474848
The Type II secretion system delivers matrix proteins for biofilm formation by Vibrio choleraeQ34593413
In vivo cross-linking of EpsG to EpsL suggests a role for EpsL as an ATPase-pseudopilin coupling protein in the Type II secretion system of Vibrio choleraeQ34605983
Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion.Q34685284
Proteomic analysis of the Vibrio cholerae type II secretome reveals new proteins, including three related serine proteasesQ34947350
A type II secreted RNase of Legionella pneumophila facilitates optimal intracellular infection of Hartmannella vermiformisQ34953095
Type II Protein Secretion in Pseudomonas aeruginosa : the Pseudopilus Is a Multifibrillar and Adhesive StructureQ34984280
Dynamic interplay between the periplasmic and transmembrane domains of GspL and GspM in the type II secretion system.Q35041385
Lcl of Legionella pneumophila is an immunogenic GAG binding adhesin that promotes interactions with lung epithelial cells and plays a crucial role in biofilm formation.Q35075799
Involvement of the GspAB complex in assembly of the type II secretion system secretin of Aeromonas and Vibrio speciesQ35095900
A metalloprotease secreted by the type II secretion system links Vibrio cholerae with collagen.Q35107296
VE-cadherin cleavage by LasB protease from Pseudomonas aeruginosa facilitates type III secretion system toxicity in endothelial cells.Q35120184
The binding of cholera toxin to the periplasmic vestibule of the type II secretion channelQ35577741
Synergistic stimulation of EpsE ATP hydrolysis by EpsL and acidic phospholipidsQ35610613
General secretion pathway (eps) genes required for toxin secretion and outer membrane biogenesis in Vibrio choleraeQ35632337
The type II secretion system and its ubiquitous lipoprotein substrate, SslE, are required for biofilm formation and virulence of enteropathogenic Escherichia coliQ36018127
Cloning and nucleotide sequence of the Vibrio cholerae hemagglutinin/protease (HA/protease) gene and construction of an HA/protease-negative strainQ36146458
YghG (GspSβ) is a novel pilot protein required for localization of the GspSβ type II secretion system secretin of enterotoxigenic Escherichia coliQ36211182
Bacterial type two secretion system secreted proteins: double-edged swords for plant pathogensQ36259452
Extracellular pullulanase of Klebsiella pneumoniae is a lipoproteinQ36297046
Genetic mapping and characterization of Pseudomonas aeruginosa mutants defective in the formation of extracellular proteinsQ36300592
Compromised outer membrane integrity in Vibrio cholerae Type II secretion mutantsQ36315378
Activation of a bacterial lipase by its chaperoneQ36370146
The Type II Secreted Lipase/Esterase LesA is a Key Virulence Factor Required for Xylella fastidiosa Pathogenesis in Grapevines.Q36453438
Lipopolysaccharide 3-deoxy-D-manno-octulosonic acid (Kdo) core determines bacterial association of secreted toxinsQ36475473
Acinetobacter baumannii Is Dependent on the Type II Secretion System and Its Substrate LipA for Lipid Utilization and In Vivo FitnessQ36575078
Direct involvement of type II secretion system in extracellular translocation of Shewanella oneidensis outer membrane cytochromes MtrC and OmcA.Q36804370
Contribution of the Twin Arginine Translocation system to the exoproteome of Pseudomonas aeruginosa.Q36986196
The type II secretion system: biogenesis, molecular architecture and mechanism.Q36995073
Docking and assembly of the type II secretion complex of Vibrio choleraeQ37191521
Translocation of a folded protein across the outer membrane in Escherichia coliQ37349168
Domain structure of secretin PulD revealed by limited proteolysis and electron microscopy.Q37350291
Enterotoxigenic Escherichia coli secretes a highly conserved mucin-degrading metalloprotease to effectively engage intestinal epithelial cells.Q37546507
Expanding Role of Type II Secretion in Bacterial Pathogenesis and BeyondQ37834796
Type II secretion system: a magic beanstalk or a protein escalatorQ38175754
Secretion of bacterial lipoproteins: through the cytoplasmic membrane, the periplasm and beyondQ38208070
Stable periplasmic secretion intermediate in the general secretory pathway of Escherichia coli.Q38322777
The Type II Secretion System of Legionella pneumophila Dampens the MyD88 and Toll-Like Receptor 2 Signaling Pathway in Infected Human MacrophagesQ38718759
An inner membrane platform in the type II secretion machinery of Gram-negative bacteriaQ42125635
Structural similarity of secretins from type II and type III secretion systemsQ42197991
Structural Basis of Type 2 Secretion System Engagement between the Inner and Outer Bacterial MembranesQ42379907
Structural and functional studies of EpsC, a crucial component of the type 2 secretion system from Vibrio choleraeQ42598405
Specific interaction between OutD, an Erwinia chrysanthemi outer membrane protein of the general secretory pathway, and secreted proteins.Q42619538
The PDZ domain of OutC and the N-terminal region of OutD determine the secretion specificity of the type II out pathway of Erwinia chrysanthemi.Q43591523
The general protein-export pathway is directly required for extracellular pullulanase secretion in Escherichia coli K12.Q43814377
Burkholderia cenocepacia zinc metalloproteases influence resistance to antimicrobial peptidesQ43828136
StcE, a metalloprotease secreted by Escherichia coli O157:H7, specifically cleaves C1 esterase inhibitor.Q44067982
Haemagglutinin/protease expression and mucin gel penetration in El Tor biotype Vibrio choleraeQ44509831
Structure of the calcium-dependent type 2 secretion pseudopilusQ45069622
Defining the interaction of the protease CpaA with its type II secretion chaperone CpaB and its contribution to virulence in Acinetobacter speciesQ46293492
The prepilin peptidase is required for protein secretion by and the virulence of the intracellular pathogen Legionella pneumophilaQ46308706
Novel mechanism of outer membrane targeting of proteins in Gram-negative bacteriaQ46471583
Export and secretion of the lipoprotein pullulanase by Klebsiella pneumoniaeQ46948520
The structure and membrane topography of the Vibrio-type secretin complex from the T2SS of enteropathogenic Escherichia coli (EPEC).Q47586784
The crystal structure of the periplasmic domain of the type II secretion system protein EpsM from Vibrio cholerae: the simplest version of the ferredoxin foldQ47772184
Stenotrophomonas maltophilia Serine Protease StmPr1 Induces Matrilysis, Anoikis, and Protease-Activated Receptor 2 Activation in Human Lung Epithelial CellsQ47820779
Genes required for extracellular secretion of enterotoxin are clustered in Vibrio choleraeQ48100741
The extracellular matrix of the oleolytic biofilms of Marinobacter hydrocarbonoclasticus comprises cytoplasmic proteins and T2SS effectors that promote growth on hydrocarbons and lipids.Q51406791
Structural insight into the assembly of the type II secretion system pilotin-secretin complex from enterotoxigenic Escherichia coli.Q52327414
Secretins revealed: structural insights into the giant gated outer membrane portals of bacteria.Q52341527
The elastase propeptide functions as an intramolecular chaperone required for elastase activity and secretion in Pseudomonas aeruginosa.Q52521406
Type II Secretion-Dependent Aminopeptidase LapA and Acyltransferase PlaC Are Redundant for Nutrient Acquisition during Legionella pneumophila Intracellular Infection of Amoebas.Q52582150
Substrate recognition by the bacterial type II secretion system: more than a simple interaction.Q53057262
CpaA a novel protease from Acinetobacter baumannii clinical isolates deregulates blood coagulation.Q53530929
Vibrio spp. secrete proaerolysin as a folded dimer without the need for disulphide bond formation.Q54604510
An enzyme with type IV prepilin peptidase activity is required to process components of the general extracellular protein secretion pathway of Klebsiella oxytocaQ54682441
C-terminal processing of GlyGly-CTERM containing proteins by rhombosortase in Vibrio choleraeQ57799425
Structure-guided disruption of the pseudopilus tip complex inhibits the Type II secretion in Pseudomonas aeruginosaQ57805452
Direct interactions between the secreted effector and the T2SS components GspL and GspM reveal a new effector-sensing step during type 2 secretionQ58597689
Cryo-EM analysis of the T3S injectisome reveals the structure of the needle and open secretinQ58705479
Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosaQ59329063
CpaA Is a Glycan-Specific Adamalysin-like Protease Secreted by Acinetobacter baumannii That Inactivates Coagulation Factor XIIQ60301194
Secreted Phosphatase and Deoxyribonuclease Are Required by Pseudomonas aeruginosa To Defend against Neutrophil Extracellular Traps.Q64996420
Processing and methylation of PuIG, a pilin-like component of the general secretory pathway of Klebsiella oxytocaQ70511675
Role of the propeptide in folding and secretion of elastase of Pseudomonas aeruginosaQ71577074
Mutual stabilization of the XcpZ and XcpY components of the secretory apparatus in Pseudomonas aeruginosaQ77781128
Interaction domains in the Pseudomonas aeruginosa type II secretory apparatus component XcpS (GspF)Q80227664
The type II secretion arrowhead: the structure of GspI-GspJ-GspKQ81227715
The X-ray structure of the type II secretion system complex formed by the N-terminal domain of EpsE and the cytoplasmic domain of EpsL of Vibrio choleraeQ81671356
Structure of a membrane-based steric chaperone in complex with its lipase substrateQ82735395
Sorting of an integral outer membrane protein via the lipoprotein-specific Lol pathway and a dedicated lipoprotein pilotinQ83454751
The type II secretion system (Xcp) of Pseudomonas putida is active and involved in the secretion of phosphatasesQ86492748
The Dynamic Structures of the Type IV PilusQ92066449
P433issue2
P407language of work or nameEnglishQ1860
P577publication date2019-02-01
P1433published inEcoSal PlusQ27725548
P1476titleArchitecture, Function, and Substrates of the Type II Secretion System
P478volume8

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cites work (P2860)
Q92540723Assessing the impact, genomics and evolution of type II secretion across a large, medically important genus: the Legionella type II secretion paradigm
Q90297938Monoderm bacteria: the new frontier for type IV pilus biology
Q97529112Structure, Dynamics and Cellular Insight Into Novel Substrates of the Legionella pneumophila Type II Secretion System
Q92576306Type II Secretion Promotes Bacterial Growth within the Legionella-Containing Vacuole in Infected Amoebae

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