Quantitation of Na+/K(+)-ATPase and glucose transporter isoforms in rat adipocyte plasma membrane by immunogold labeling

scientific article published on 01 October 1993

Quantitation of Na+/K(+)-ATPase and glucose transporter isoforms in rat adipocyte plasma membrane by immunogold labeling is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1007/BF00241490
P2888exact matchhttps://scigraph.springernature.com/pub.10.1007/bf00241490
P698PubMed publication ID8271273

P2093author name stringJ Vinten
M Voldstedlund
J Tranum-Jensen
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A simplification of the protein assay method of Lowry et al. which is more generally applicableQ29615220
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Intracellular targeting of the insulin-regulatable glucose transporter (GLUT4) is isoform specific and independent of cell typeQ33235315
Histochemical localization of Na+,K+-ATPaseQ69826478
Ouabain sensitivity of the alpha 3 isozyme of rat Na,K-ATPaseQ69842828
Insulin affects the sodium affinity of the rat adipocyte (Na+,K+)-ATPaseQ70093144
Quantitation and characterization of the (Na+,K+)-adenosine triphosphatase in the rat adipocyte plasma membraneQ72951346
Potocytosis: Sequestration and Transport of Small Molecules by CaveolaeQ34233280
Differential expression of Na+,K+-ATPase alpha- and beta-subunit mRNAs in rat tissues and cell linesQ34378447
Preparation and characterization of a plasma membrane fraction from isolated fat cellsQ36191508
Three differentially expressed Na,K-ATPase alpha subunit isoforms: structural and functional implicationsQ36216926
Evidence that insulin causes translocation of glucose transport activity to the plasma membrane from an intracellular storage siteQ36387605
Functional significance of the oligomeric structure of the Na,K-pump from radiation inactivation and ligand bindingQ36422190
Immunoelectron microscopic demonstration of insulin-stimulated translocation of glucose transporters to the plasma membrane of isolated rat adipocytes and masking of the carboxyl-terminal epitope of intracellular GLUT4.Q37569047
Isozymes of the Na+/K+-ATPaseQ38270644
The Na,K-pumpQ39207055
Regulation of active Na+-K+ transport in skeletal muscleQ39503777
Digitonin-induced changes in subunit arrangement in relation to some in vitro activities of the (Na+,K+)-ATPaseQ39831865
The number of sodium ion pumping sites in skeletal muscle and its modification by insulinQ40009426
Colloidal gold : a cytochemical marker for light and fluorescent microscopy and for transmission and scanning electron microscopyQ40322848
Insulin activation of (sodium, potassium)-adenosine triphosphatase exhibits a temperature-dependent lag time. Comparison to activation of the glucose transporterQ41556719
Na(+)-K(+)-ATPase in adipocyte differentiation in cultureQ41712446
Characterization of the (Na+ (+) K+)-ATPase from 3T3-F442A fibroblasts and adipocytes. Isozymes and insulin sensitivityQ41730487
Insulin stimulates the translocation of Na+/K(+)-dependent ATPase molecules from intracellular stores to the plasma membrane in frog skeletal muscleQ42162013
Insulin stimulation of (Na+,K+)-adenosine triphosphatase-dependent 86Rb+ uptake in rat adipocytesQ42450975
Development of insulin responsiveness of the glucose transporter and the (Na+,K+)-adenosine triphosphatase during in vitro adipocyte differentiationQ42452136
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Fine specificity mapping and topography of an isozyme-specific epitope of the Na,K-ATPase catalytic subunit.Q44156453
A substantial part of GLUT-1 in crude membranes from muscle originates from perineurial sheathsQ44442170
Active Na-K transport and the rate of ouabain binding. The effect of insulin and other stimuli on skeletal muscle and adipocytesQ44587187
Exchange of 3-O-methylglucose in isolated fat cells. Concentration dependence and effect of insulinQ45038041
Estimation of Na,K-pump numbers and turnover in intact cells with [3H]ouabainQ45148607
Membrane-bound Na,K-ATPase: target size and radiation inactivation size of some of its enzymatic reactionsQ47176004
Developmental and thyroid hormone regulation of two molecular forms of Na+-K+-ATPase in brain.Q48311840
Identification of two molecular forms of (Na+,K+)-ATPase in rat adipocytes. Relation to insulin stimulation of the enzyme.Q48534787
Insulin activation of brain Na(+)-K(+)-ATPase is mediated by alpha 2-form of enzyme.Q49010378
A new method of preparing gold probes for multiple-labeling cytochemistry.Q49320313
Application of the nitrocellulose transfer technique and alkaline phosphatase conjugated anti-immunoglobulin for determination of the specificity of monoclonal antibodies to protein mixtures.Q50213024
Identification of three isozyme proteins of the catalytic subunit of the Na,K-ATPase in rat brain.Q52246333
Coupling polylysine to glass beads for plasma membrane isolationQ67248251
Effects of hypokalemia on the properties and expression of the (Na+,K+)-ATPase of rat skeletal muscleQ67678119
Insulin induces translocation of the alpha 2 and beta 1 subunits of the Na+/K(+)-ATPase from intracellular compartments to the plasma membrane in mammalian skeletal muscleQ67842358
Phosphate binding and ATP-binding sites coexist in Na+/K(+)-transporting ATPase, as demonstrated by the inactivating MgPO4 complex analogue Co(NH3)4PO4Q67913531
Electron microscopy: assays involving negative stainingQ68041286
Characterization of the adipocyte ghost (Na+,K+) pump. Insights into the insulin regulation of the adipocyte (Na+,K+) pumpQ68249840
Insulin stimulates both the alpha 1 and the alpha 2 isoforms of the rat adipocyte (Na+,K+) ATPase. Two mechanisms of stimulationQ68249844
Quantitative aspects of immunogold labeling in embedded and in nonembedded sectionsQ69097731
Identification and quantitation of Na+,K+-ATPase by back-door phosphorylationQ69826421
Determination of quaternary structure of an active enzyme using chemical cross-linking with glutaraldehydeQ69826461
Preparation and use of monoclonal antibodies to Na+,K+-ATPaseQ69826475
P433issue1
P921main subjectadipocyteQ357519
P1104number of pages11
P304page(s)63-73
P577publication date1993-10-01
P1433published inJournal of Membrane BiologyQ6295550
P1476titleQuantitation of Na+/K(+)-ATPase and glucose transporter isoforms in rat adipocyte plasma membrane by immunogold labeling
P478volume136

Reverse relations

cites work (P2860)
Q35010401Caveolae and lipid trafficking in adipocytes
Q42814557Caveolin-associated filamentous actin (Cav-actin) defines a novel F-actin structure in adipocytes
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Q57364636Chapter 8 Insulin Signaling and Caveolae
Q24295215Colocalization of insulin receptor and insulin receptor substrate-1 to caveolae in primary human adipocytes. Cholesterol depletion blocks insulin signalling for metabolic and mitogenic control
Q34559663Compartmentalization of the exocyst complex in lipid rafts controls Glut4 vesicle tethering
Q42443596Differential protein expression in lens epithelial whole-mounts and lens epithelial cell cultures
Q36235656Expression and compartmentalization of caveolin in adipose cells: coordinate regulation with and structural segregation from GLUT4.
Q36436241Insulin stimulates transepithelial sodium transport by activation of a protein phosphatase that increases Na-K ATPase activity in endometrial epithelial cells
Q39127657Intense exercise up-regulates Na+,K+-ATPase isoform mRNA, but not protein expression in human skeletal muscle.
Q30441963Lipid raft microdomain compartmentalization of TC10 is required for insulin signaling and GLUT4 translocation
Q35541873Na+-K+Pump Regulation and Skeletal Muscle Contractility
Q28506688Spatial and temporal regulation of GLUT4 translocation by flotillin-1 and caveolin-3 in skeletal muscle cells
Q28573732Vp165 and GLUT4 share similar vesicle pools along their trafficking pathways in rat adipose cells

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