Reevaluating the effect of Brefeldin A (BFA) on ganglioside synthesis: the location of GM2 synthase cannot be deduced from the inhibition of GM2 synthesis by BFA

scientific article published on 01 July 1999

Reevaluating the effect of Brefeldin A (BFA) on ganglioside synthesis: the location of GM2 synthase cannot be deduced from the inhibition of GM2 synthesis by BFA is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1093/GLYCOB/9.7.689
P698PubMed publication ID10362838

P2093author name stringW W Young
M L Allende
E Jaskiewicz
P2860cites workBrefeldin A induced inhibition of de novo globo- and neolacto-series glycolipid core chain biosynthesis in human cells. Evidence for an effect on beta 1-->4galactosyltransferase activityQ45902699
Golgi proteins persist in the tubulovesicular remnants found in brefeldin A-treated pancreatic acinar cellsQ46485995
Two soluble glycosyltransferases glycosylate less efficiently in vivo than their membrane bound counterpartsQ47929132
Uncoupling of ganglioside biosynthesis by Brefeldin A.Q49108130
Topology of glucosylceramide synthesis in Golgi membranes from porcine submaxillary glands.Q52489131
De novo synthesis of type 1 lacto-series glycolipids in human colonic adenocarcinoma cells: efficient synthesis of the Le(a) antigen and absence of brefeldin A-induced inhibition of its synthesis in Colo 205 cells.Q52516733
Inhibition by brefeldin A of a Golgi membrane enzyme that catalyses exchange of guanine nucleotide bound to ARFQ59066677
Biosynthesis of brain gangliosidesQ67752863
Presence of Golgi remnant membranes in the cytoplasm of brefeldin A-treated cellsQ67922898
Sub-Golgi distribution in rat liver of CMP-NeuAc GM3- and CMP-NeuAc:GT1b alpha 2----8sialyltransferases and comparison with the distribution of the other glycosyltransferase activities involved in ganglioside biosynthesisQ68358980
pH-dependent changes of ganglioside biosynthesis in neuronal cell cultureQ68380499
Two glycosphingolipid sialyltransferases are localized in different sub-Golgi compartments in rat liverQ69723204
Differential subcompartmentation of terminal glycosylation in the Golgi apparatus of intestinal absorptive and goblet cellsQ70309397
Cloned beta 1,4N-acetylgalactosaminyltransferase: subcellular localization and formation of disulfide bonded speciesQ71338765
Effects of brefeldin A on synthesis and intracellular transport of ganglioside GT3 by chick embryo retina cellsQ71748817
Pathways of glycosphingolipid biosynthesis in SW13 cells in the presence and absence of vimentin intermediate filamentsQ71964088
Effect of brefeldin A on ganglioside metabolism in cultured neurons: implications for the intracellular traffic of gangliosidesQ72352077
Differential effect of brefeldin A on the biosynthesis of heparan sulfate and chondroitin/dermatan sulfate proteoglycans in rat ovarian granulosa cells in cultureQ72875773
Beta 1,4 N-acetylgalactosaminyltransferase (GM2/GD2/GA2 synthase) forms homodimers in the endoplasmic reticulum: a strategy to test for dimerization of Golgi membrane proteinsQ73861325
Functional organization of the Golgi apparatus in glycosphingolipid biosynthesis. Lactosylceramide and subsequent glycosphingolipids are formed in the lumen of the late GolgiQ74106011
The role of sphingolipids in the process of signal transductionQ74640255
Expression cloning of beta 1,4 N-acetylgalactosaminyltransferase cDNAs that determine the expression of GM2 and GD2 gangliosidesQ24307744
Measurement of protein using bicinchoninic acidQ26778491
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene productQ27860841
Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activityQ27935634
Assembly of asparagine-linked oligosaccharidesQ28131690
Localization of three human polypeptide GalNAc-transferases in HeLa cells suggests initiation of O-linked glycosylation throughout the Golgi apparatusQ28256097
ADP-ribosylation factor, a small GTP-binding protein, is required for binding of the coatomer protein beta-COP to Golgi membranesQ28609784
Use of brefeldin A to define sites of glycosphingolipid synthesis: GA2/GM2/GD2 synthase is trans to the brefeldin A blockQ30452579
Phospholipase D is present on Golgi-enriched membranes and its activation by ADP ribosylation factor is sensitive to brefeldin AQ34277362
Purification and characterization of UDP-N-acetylgalactosamine GM3/GD3 N-acetylgalactosaminyltransferase from mouse liverQ34345028
Beta1,4-N-acetylgalactosaminyltransferase (GM2 synthase) is released from Golgi membranes as a neuraminidase-sensitive, disulfide-bonded dimer by a cathepsin D-like proteaseQ34398985
Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteinsQ34470955
Regulation of glycoprotein biosynthesis by formation of specific glycosyltransferase complexesQ35392928
Compartmentation of asparagine-linked oligosaccharide processing in the Golgi apparatusQ36207471
Compartmentation of the Golgi complex: brefeldin-A distinguishes trans-Golgi cisternae from the trans-Golgi networkQ36223809
Overlapping distribution of two glycosyltransferases in the Golgi apparatus of HeLa cellsQ36232261
Retention of p63 in an ER-Golgi intermediate compartment depends on the presence of all three of its domains and on its ability to form oligomersQ36234357
Golgi tubule traffic and the effects of brefeldin A visualized in living cellsQ36275025
Brefeldin A and the endocytic pathway. Possible implications for membrane traffic and sorting.Q36360101
O-glycosylation of intact and truncated ribophorins in brefeldin A-treated cells: newly synthesized intact ribophorins are only transiently accessible to the relocated glycosyltransferasesQ36531451
Subcellular organization of glycosylation in mammalian cellsQ39668642
Compartmental organization of the Golgi stackQ39847933
Molecular approaches to studying the intracellular trafficking of glycosphingolipidsQ40850149
Effects of brefeldin A on galactosphingolipid synthesis in an immortalized Schwann cell line: evidence for different intracellular locations of galactosylceramide sulfotransferase and ceramide galactosyltransferase activitiesQ41290900
Localization of O-glycan initiation, sphingomyelin synthesis, and glucosylceramide synthesis in Vero cells with respect to the endoplasmic reticulum-Golgi intermediate compartmentQ41487976
The trans-Golgi network can be dissected structurally and functionally from the cisternae of the Golgi complex by brefeldin AQ41599650
Mechanism for the biosynthesis of Forssman glycolipid from trihexosylceramideQ41599658
O-glycosylation of the coronavirus M protein. Differential localization of sialyltransferases in N- and O-linked glycosylationQ41613514
Post-Golgi membrane traffic: brefeldin A inhibits export from distal Golgi compartments to the cell surface but not recyclingQ41614909
Glucosylceramide is synthesized at the cytosolic surface of various Golgi subfractionsQ41632006
Differential effects of brefeldin A on sialylation of N- and O-linked oligosaccharides in low density lipoprotein receptor and epidermal growth factor receptor.Q41718086
Determination of the intracellular sites and topology of glucosylceramide synthesis in rat liverQ41950312
A Golgi-related structure remains after the brefeldin A-induced formation of an ER-Golgi hybrid compartmentQ41996274
Isolation and characterization of mutant CHO cell lines with compartment-specific resistance to brefeldin A.Q42770736
The spectrum of incomplete N-linked oligosaccharides synthesized by endothelial cells in the presence of brefeldin A.Q43503848
Biosynthesis of sphingolipids: dihydroceramide and not sphinganine is desaturated by cultured cellsQ44313132
The synthesis of complex carbohydrates by multiglycosyltransferase systems and their potential function in intercellular adhesionQ44473935
Subcellular localization and membrane topology of serine palmitoyltransferase, 3-dehydrosphinganine reductase, and sphinganine N-acyltransferase in mouse liverQ45365052
P433issue7
P304page(s)689-695
P577publication date1999-07-01
P1433published inGlycobiologyQ5572596
P1476titleReevaluating the effect of Brefeldin A (BFA) on ganglioside synthesis: the location of GM2 synthase cannot be deduced from the inhibition of GM2 synthesis by BFA
P478volume9