The three-dimensional structure of in vitro reconstituted Xenopus laevis chromosomes by EM tomography

scientific article published on 28 February 2007

The three-dimensional structure of in vitro reconstituted Xenopus laevis chromosomes by EM tomography is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1007/S00412-007-0101-0
P698PubMed publication ID17333236

P50authorPeter KönigQ41048973
P2093author name stringDavid A Agard
John W Sedat
Michael B Braunfeld
P2860cites workCrystal structure of the nucleosome core particle at 2.8 A resolutionQ22122355
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Differential contributions of condensin I and condensin II to mitotic chromosome architecture in vertebrate cellsQ24297107
EM measurements define the dimensions of the "30-nm" chromatin fiber: evidence for a compact, interdigitated structureQ24542512
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Nucleosomes, linker DNA, and linker histone form a unique structural motif that directs the higher-order folding and compaction of chromatinQ24652372
Histone H1 is essential for mitotic chromosome architecture and segregation in Xenopus laevis egg extractsQ24678815
Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatinQ24681606
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Crystal structure of the SMC head domain: an ABC ATPase with 900 residues antiparallel coiled-coil insertedQ27629557
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Condensin binding at distinct and specific chromosomal sites in the Saccharomyces cerevisiae genomeQ27930572
Structural maintenance of chromosomes protein C-terminal domains bind preferentially to DNA with secondary structureQ27937303
Condensin and cohesin display different arm conformations with characteristic hinge anglesQ28216842
Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren proteinQ28238784
Chromosome structure: improved immunolabeling for electron microscopyQ28273312
Phosphorylation and activation of 13S condensin by Cdc2 in vitroQ28285478
ELECTRON STAINS: I. Chemical Studies on the Interaction of DNA with Uranyl SaltsQ29041571
Reorganization of chromatin in Xenopus egg extracts: electron microscopic studiesQ33183235
Direct detection of linker DNA bending in defined-length oligomers of chromatinQ33821984
Cryo-electron microscopy of vitrified chromosomes in situ.Q33879953
Structures and interactions of the core histone tail domainsQ34187133
A model for chromosome structure during the mitotic and meiotic cell cyclesQ34237078
Assembly of chromatin fibers into metaphase chromosomes analyzed by transmission electron microscopy and scanning electron microscopyQ34258032
Proteolysis of mitotic chromosomes induces gradual and anisotropic decondensation correlated with a reduction of elastic modulus and structural sensitivity to rarely cutting restriction enzymesQ34298302
Automated electron microscope tomography of frozen-hydrated chromatin: the irregular three-dimensional zigzag architecture persists in compact, isolated fibersQ39459139
Condensin but not cohesin SMC heterodimer induces DNA reannealing through protein-protein assemblyQ39758831
Deoxyribonucleic acid loop domain tertiary structure in mammalian spermatozoaQ40819404
The distribution of topoisomerase II on mammalian chromosomesQ41252778
Use of surface affinity enrichment and cryo-embedding to prepare in vitro reconstituted mitotic chromosomes for EM tomographyQ41627285
Histone H1 and chromatin higher-order structureQ41661744
Modulation of the higher-order folding of chromatin by deletion of histone H3 and H4 terminal domains.Q42982373
Nucleoplasmin remodels sperm chromatin in Xenopus egg extractsQ43443780
Efficient supercoiling of DNA by a single condensin complex as revealed by electron spectroscopic imagingQ44042955
A Two-Step Scaffolding Model for Mitotic Chromosome AssemblyQ44399616
Chromatin assemblyQ44405396
Multiple chromosomal populations of topoisomerase II detected in vivo by time-lapse, three-dimensional wide-field microscopyQ46125653
Large-scale chromatin structural domains within mitotic and interphase chromosomes in vivo and in vitroQ46871282
Toward fully automated high-resolution electron tomographyQ46892441
The mitotic chromosome is an assembly of rigid elastic axes organized by structural maintenance of chromosomes (SMC) proteins and surrounded by a soft chromatin envelopeQ47336365
Chromosome length and DNA loop size during early embryonic development of Xenopus laevis.Q47391022
Dinucleosomes show compaction by ionic strength, consistent with bending of linker DNA.Q47708879
Chromosome condensation in Xenopus mitotic extracts without histone H1.Q52220944
Three-dimensional reconstruction of a human metaphase chromosome from electron micrographs.Q52609938
Disassembly of the mammalian metaphase chromosome into its subunits: studies with ultraviolet light and repair synthesis inhibitors.Q53531369
Radial loops and helical coils coexist in metaphase chromosomes.Q53703047
High-order structure of metaphase chromosomes: evidence for a multiple coiling modelQ69567736
Metaphase chromosome structure. Involvement of topoisomerase IIQ70144641
Transitions between in situ and isolated chromatinQ70501386
Subdivision of the mitotic cycle into eleven stages, on the basis of the chromosomal changes observed in mouse duodenal crypt cells stained by the DNA-specific Feulgen reactionQ71622963
Globular and fibrous structure in barley chromosomes revealed by high-resolution scanning electron microscopyQ73685817
Resolving the role of topoisomerase II in chromatin structure and functionQ75294399
Spatial and temporal regulation of Condensins I and II in mitotic chromosome assembly in human cells.Q34321072
Nucleosome arrays reveal the two-start organization of the chromatin fiberQ34371762
Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffoldQ34386361
X-ray structure of a tetranucleosome and its implications for the chromatin fibreQ34431881
DNA renaturation activity of the SMC complex implicated in chromosome condensationQ34438003
ATP-dependent positive supercoiling of DNA by 13S condensin: a biochemical implication for chromosome condensationQ34438428
13S condensin actively reconfigures DNA by introducing global positive writhe: implications for chromosome condensationQ34504401
The making of the mitotic chromosome: modern insights into classical questionsQ34532415
Condensin is required for nonhistone protein assembly and structural integrity of vertebrate mitotic chromosomesQ34536233
Visualization of early chromosome condensation: a hierarchical folding, axial glue model of chromosome structureQ34550665
Cell cycle extractsQ34590228
A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitroQ34725116
Mitotic chromosome condensation.Q34737023
Phosphorylation of serine 10 in histone H3, what for?Q35199083
From DNA structure to gene expression: mediators of nuclear compartmentalization and dynamics.Q35217751
Dual roles of the 11S regulatory subcomplex in condensin functionsQ35359850
Intercalary heterochromatin and genetic silencingQ35568150
Multiple roles of Condensins: a complex story.Q35795403
Visualization of nucleosomes in thin sections by stereo electron microscopy.Q36202003
Localization of topoisomerase II in mitotic chromosomesQ36213345
A three-dimensional approach to mitotic chromosome structure: evidence for a complex hierarchical organizationQ36218032
Topoisomerase II does not play a scaffolding role in the organization of mitotic chromosomes assembled in Xenopus egg extractsQ36232430
The three-dimensional architecture of chromatin in situ: electron tomography reveals fibers composed of a continuously variable zig-zag nucleosomal ribbonQ36234105
Elasticity measurements show the existence of thin rigid cores inside mitotic chromosomes.Q36256431
A role of topoisomerase II in linking DNA replication to chromosome condensationQ36324263
Role of linker histone in chromatin structure and function: H1 stoichiometry and nucleosome repeat lengthQ36408581
Stereoscopic scanning electron microscopy of the chromosomes in Vicia faba (broad beans).Q36632995
Nucleosome arcs and helicesQ36638552
Topoisomerase II forms multimers in vitro: effects of metals, beta-glycerophosphate, and phosphorylation of its C-terminal domainQ36668278
DNA changes involved in the formation of metaphase chromosomes, as observed in mouse duodenal crypt cells stained by osmium-ammine. I. New structures arise during the S phase and condense at prophase into "chromomeres," which fuse at prometaphase inQ36670730
IVE (Image Visualization Environment): a software platform for all three-dimensional microscopy applicationsQ36811607
Nucleosome positioning is determined by the (H3-H4)2 tetramerQ37631358
Mammalian SMC3 C-terminal and coiled-coil protein domains specifically bind palindromic DNA, do not block DNA ends, and prevent DNA bendingQ38317132
Structure of nucleosome core particles of chromatinQ38560180
P433issue4
P921main subjectAfrican clawed frogQ654718
tomographyQ841267
P304page(s)349-372
P577publication date2007-02-28
P1433published inChromosomaQ15765851
P1476titleThe three-dimensional structure of in vitro reconstituted Xenopus laevis chromosomes by EM tomography
P478volume116

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cites work (P2860)
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