Membrane directed expression in Escherichia coli of BBA57 and other virulence factors from the Lyme disease agent Borrelia burgdorferi

scientific article published on 26 November 2019

Membrane directed expression in Escherichia coli of BBA57 and other virulence factors from the Lyme disease agent Borrelia burgdorferi is …
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scholarly articleQ13442814

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P356DOI10.1038/S41598-019-53830-X
P932PMC publication ID6879480
P698PubMed publication ID31772280

P50authorDebra Tumbula-HansenQ56878997
Po-Lin ChiuQ41604527
P2093author name stringPetra Fromme
Felicia M Craciunescu
Po-Lin Chiu
Chloe D Truong
Karie E Robertson
P2860cites workProtection of mice against the Lyme disease agent by immunizing with recombinant OspAQ68598312
Borrelia burgdorferi surface-located Lmp1 protein processed into region-specific polypeptides that are critical for microbial persistenceQ88635477
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Crystal structure of the N-terminal domain of the major virulence factor BB0323 from the Lyme disease agent Borrelia burgdorferiQ93071002
Elimination of channel-forming activity by insertional inactivation of thep66gene inBorrelia burgdorferiQ57792715
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Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferiQ22122357
Borrelia burgdorferi OspC protein required exclusively in a crucial early stage of mammalian infectionQ24548272
A chromosomally encoded virulence factor protects the Lyme disease pathogen against host-adaptive immunityQ27317412
Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein exportQ27625163
Structure of a potentially open state of a proton-activated pentameric ligand-gated ion channelQ27652787
Structural basis for iron piracy by pathogenic NeisseriaQ27677223
Structural basis for the antifolding activity of a molecular chaperoneQ27726706
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoterQ27860697
Predicting transmembrane protein topology with a hidden Markov model: application to complete genomesQ27860838
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The Lyme disease agent exploits a tick protein to infect the mammalian hostQ28264022
Principles governing amino acid composition of integral membrane proteins: application to topology predictionQ28285162
TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferiQ28292578
An RND-type efflux system in Borrelia burgdorferi is involved in virulence and resistance to antimicrobial compoundsQ28472526
Lipid exchange between Borrelia burgdorferi and host cellsQ28484938
Lyme borreliosisQ29013670
Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levelsQ29547813
The modular nature of the β-barrel assembly machinery, illustrated in Borrelia burgdorferiQ30152728
The TamB ortholog of Borrelia burgdorferi interacts with the β-barrel assembly machine (BAM) complex protein BamAQ30152732
Consensus computational network analysis for identifying candidate outer membrane proteins from Borrelia spirochetesQ30152759
Outer membrane lipoprotein biogenesis: Lol is not the end.Q30152837
Characterization of the β-barrel assembly machine accessory lipoproteins from Borrelia burgdorferiQ30152946
Structural and mechanistic insights into the bacterial amyloid secretion channel CsgGQ30153336
Structural modeling and physicochemical characterization provide evidence that P66 forms a β-barrel in the Borrelia burgdorferi outer membraneQ30153471
A tag at the carboxy terminus prevents membrane integration of VDAC1 in mammalian mitochondriaQ30156973
Structure of the membrane protein FhaC: a member of the Omp85-TpsB transporter superfamily.Q30157890
Intact flagellar motor of Borrelia burgdorferi revealed by cryo-electron tomography: evidence for stator ring curvature and rotor/C-ring assembly flexion.Q30489481
Characterization of a candidate Borrelia burgdorferi beta3-chain integrin ligand identified using a phage display libraryQ30826852
Pleiotropic effects of inactivating a carboxyl-terminal protease, CtpA, in Borrelia burgdorferiQ33199752
Detergent binding explains anomalous SDS-PAGE migration of membrane proteinsQ33404282
P13, an integral membrane protein of Borrelia burgdorferi, is C-terminally processed and contains surface-exposed domainsQ34007376
Targeted mutation of the outer membrane protein P66 disrupts attachment of the Lyme disease agent, Borrelia burgdorferi , to integrin α v β 3Q34197056
DipA, a pore-forming protein in the outer membrane of Lyme disease spirochetes exhibits specificity for the permeation of dicarboxylatesQ34270800
Identification of Borrelia burgdorferi outer surface proteinsQ34300931
Cholesterol lipids of Borrelia burgdorferi form lipid rafts and are required for the bactericidal activity of a complement-independent antibody.Q34443882
ActA promotes Listeria monocytogenes aggregation, intestinal colonization and carriageQ34574836
Wza the translocon for E. coli capsular polysaccharides defines a new class of membrane proteinQ34579482
BB0323 and novel virulence determinant BB0238: Borrelia burgdorferi proteins that interact with and stabilize each other and are critical for infectivityQ35152795
Characterization of multiprotein complexes of the Borrelia burgdorferi outer membrane vesicles.Q35291688
Porin activity of the native and recombinant outer membrane protein Oms28 of Borrelia burgdorferiQ35611029
Virulent strain associated outer membrane proteins of Borrelia burgdorferiQ35766458
A rapid and highly efficient method for preparation of competent Escherichia coli cellsQ35883764
OspC is potent plasminogen receptor on surface of Borrelia burgdorferi.Q35956516
The role of Borrelia burgdorferi outer surface proteinsQ36179733
The β₃-integrin ligand of Borrelia burgdorferi is critical for infection of mice but not ticksQ36223580
Outer Membrane BiogenesisQ47833019
Decorin-binding adhesins from Borrelia burgdorferiQ47977071
Structural basis for chitin acquisition by marine Vibrio speciesQ48240135
Borrelia burgdorferi surface protein Lmp1 facilitates pathogen dissemination through ticks as studied by an artificial membrane feeding systemQ49182258
A multiplex serologic platform for diagnosis of tick-borne diseasesQ49912320
Plasticity in early immune evasion strategies of a bacterial pathogen.Q52611917
Extracellular HtrA serine proteases: An emerging new strategy in bacterial pathogenesis.Q52690250
Optimizing membrane protein overexpression in the Escherichia coli strain Lemo21(DE3).Q54331016
Enhancing the secretory yields of leech carboxypeptidase inhibitor in Escherichia coli: influence of trigger factor and signal recognition particle.Q54383219
Lyme borreliosisQ56764150
Characterization of recombinant OspA in two different Borrelia vaccines with respect to immunological response and its relationship to functional parametersQ57456083
Comprehensive Spatial Analysis of the Borrelia burgdorferi Lipoproteome Reveals a Compartmentalization Bias toward the Bacterial SurfaceQ36245018
A high-throughput genetic screen identifies previously uncharacterized Borrelia burgdorferi genes important for resistance against reactive oxygen and nitrogen species.Q36284155
Essential role for OspA/B in the life cycle of the Lyme disease spirocheteQ36403858
Middle region of the Borrelia burgdorferi surface-located protein 1 (Lmp1) interacts with host chondroitin-6-sulfate and independently facilitates infectionQ36480061
Novel microbial virulence factor triggers murine lyme arthritisQ36607282
Solubilization of the Cytoplasmic Membrane of Escherichia coli by the Ionic Detergent Sodium-Lauryl SarcosinateQ36765064
Proteolysis of BB0323 results in two polypeptides that impact physiologic and infectious phenotypes in Borrelia burgdorferiQ36788005
A genome-wide proteome array reveals a limited set of immunogens in natural infections of humans and white-footed mice with Borrelia burgdorferiQ36804305
A Borrelia burgdorferi Surface-Exposed Transmembrane Protein Lacking Detectable Immune Responses Supports Pathogen Persistence and Constitutes a Vaccine TargetQ36871934
Absence of lipopolysaccharide in the Lyme disease spirochete, Borrelia burgdorferiQ37023391
Borrelia burgdorferi HtrA: evidence for twofold proteolysis of outer membrane protein p66.Q37217348
The bactericidal effect of a complement-independent antibody is osmolytic and specific to BorreliaQ37249986
Borrelia burgdorferi RevA antigen binds host fibronectinQ37256541
Protein oligomerization in the bacterial outer membrane (Review).Q37395930
Control of the negative IRES trans-acting factor KHSRP by ubiquitinationQ37577374
Outer Membrane Proteins BB0405 and BB0406 Are Immunogenic, but Only BB0405 Is Required for Borrelia burgdorferi InfectionQ37611969
Purification and biophysical characterization of the CapA membrane protein FTT0807 from Francisella tularensisQ37701342
Protein quality control in the bacterial periplasmQ37884601
A phylum level analysis reveals lipoprotein biosynthesis to be a fundamental property of bacteriaQ37992840
Protein translocation across the inner membrane of Gram-negative bacteria: the Sec and Tat dependent protein transport pathways.Q38097261
Outer surface proteins of Borrelia: peerless immune evasion toolsQ38189871
Secretion of bacterial lipoproteins: through the cytoplasmic membrane, the periplasm and beyondQ38208070
Epidemiology of Lyme diseaseQ38496878
Surface-Exposed Lipoproteins: An Emerging Secretion Phenomenon in Gram-Negative BacteriaQ38681733
Cholesterol lipids and cholesterol-containing lipid rafts in bacteriaQ38768859
Integrin binding by Borrelia burgdorferi P66 facilitates dissemination but is not required for infectivity.Q38918279
Targeting and Insertion of Membrane ProteinsQ39170020
Neuroborreliosis during relapsing fever: review of the clinical manifestations, pathology, and treatment of infections in humans and experimental animals.Q40642526
The presence of N-terminal secretion signal sequences leads to strong stimulation of the total expression levels of three tested medically important proteins during high-cell-density cultivations of Escherichia coliQ41810017
Both decorin-binding proteins A and B are critical for the overall virulence of Borrelia burgdorferiQ41960261
The major outer sheath protein forms distinct conformers and multimeric complexes in the outer membrane and periplasm of Treponema denticolaQ42378801
Analysis of the RpoS regulon in Borrelia burgdorferi in response to mammalian host signals provides insight into RpoS function during the enzootic cycleQ42392366
Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator-repressor interactionsQ42643060
Study of the protein complex, pore diameter, and pore-forming activity of the Borrelia burgdorferi P13 porinQ42720743
Pulling the trigger on lyme arthritisQ43248458
Translocation of group 1 capsular polysaccharide in Escherichia coli serotype K30. Structural and functional analysis of the outer membrane lipoprotein Wza.Q44605184
Structural basis for maintenance of bacterial outer membrane lipid asymmetryQ47643300
P2507corrigendum / erratumAuthor Correction: Membrane directed expression in Escherichia coli of BBA57 and other virulence factors from the Lyme disease agent Borrelia burgdorferiQ89983710
P433issue1
P921main subjectBorrelia burgdorferiQ2723634
Lyme diseaseQ201989
Escherichia coliQ25419
P304page(s)17606
P577publication date2019-11-26
P1433published inScientific ReportsQ2261792
P1476titleMembrane directed expression in Escherichia coli of BBA57 and other virulence factors from the Lyme disease agent Borrelia burgdorferi
P478volume9