Transposon Mutagenesis in Chlamydia trachomatis Identifies CT339 as a ComEC Homolog Important for DNA Uptake and Lateral Gene Transfer

scientific article published on 06 August 2019

Transposon Mutagenesis in Chlamydia trachomatis Identifies CT339 as a ComEC Homolog Important for DNA Uptake and Lateral Gene Transfer is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1128/MBIO.01343-19
P932PMC publication ID6686042
P698PubMed publication ID31387908

P50authorKelly HarrisonQ85989885
P2093author name stringP Scott Hefty
Robert J Suchland
Jason Wickstrum
Scott D LaBrie
Srishti Baid
Zoë E Dimond
P2860cites workGenome Sequence of an Obligate Intracellular Pathogen of Humans: Chlamydia trachomatisQ22065557
SMART, a simple modular architecture research tool: identification of signaling domainsQ24597380
A Coming of Age Story: Chlamydia in the Post-Genetic EraQ26775890
Genetic systems for studying obligate intracellular pathogens: an updateQ27005867
Conditional gene expression in Chlamydia trachomatis using the tet systemQ27304592
Predicting transmembrane protein topology with a hidden Markov model: application to complete genomesQ27860838
The COG database: a tool for genome-scale analysis of protein functions and evolutionQ27936664
The HMMTOP transmembrane topology prediction serverQ28190632
PSORTb v.2.0: expanded prediction of bacterial protein subcellular localization and insights gained from comparative proteome analysisQ28289386
ChlaDub1 of Chlamydia trachomatis suppresses NF-kappaB activation and inhibits IkappaBalpha ubiquitination and degradationQ28484738
Chlamydia trachomatis-derived deubiquitinating enzymes in mammalian cells during infectionQ28484743
Identification of OmpA, a Coxiella burnetii protein involved in host cell invasion, by multi-phenotypic high-content screeningQ28541251
Integrating chemical mutagenesis and whole-genome sequencing as a platform for forward and reverse genetic analysis of ChlamydiaQ28791052
Genetic requirements for mycobacterial survival during infectionQ29547599
TopPred II: an improved software for membrane protein structure predictionsQ29618438
Protein annotation and modelling servers at University College LondonQ30389763
Interplay of recombination and selection in the genomes of Chlamydia trachomatisQ30501790
Targeted and random mutagenesis of Ehrlichia chaffeensis for the identification of genes required for in vivo infectionQ31112080
Co-evolution of genomes and plasmids within Chlamydia trachomatis and the emergence in Sweden of a new variant strainQ33449883
Deep sequencing-based discovery of the Chlamydia trachomatis transcriptomeQ33633681
Identification of metabolic pathways essential for fitness of Salmonella Typhimurium in vivoQ33845728
Genome sequencing of recent clinical Chlamydia trachomatis strains identifies loci associated with tissue tropism and regions of apparent recombinationQ33877047
Development of a transformation system for Chlamydia trachomatis: restoration of glycogen biosynthesis by acquisition of a plasmid shuttle vectorQ34037667
Recent advances in large-scale transposon mutagenesisQ34134782
Evidence that CT694 is a novel Chlamydia trachomatis T3S substrate capable of functioning during invasion or early cycle developmentQ34383783
Bacterial transformation: distribution, shared mechanisms and divergent controlQ34403233
Recombination in the genome of Chlamydia trachomatis involving the polymorphic membrane protein C gene relative to ompA and evidence for horizontal gene transferQ34435137
Evidence for naturally occurring recombination in the gene encoding the major outer membrane protein of lymphogranuloma venereum isolates of Chlamydia trachomatis.Q34549416
Hyperactive Himar1 transposase mediates transposition in cell culture and enhances gene expression in vivoQ34567719
Genomic and phenotypic characterization of in vitro-generated Chlamydia trachomatis recombinantsQ34779267
Generation of targeted Chlamydia trachomatis null mutants.Q34879947
Global Stage-Specific Gene Regulation during the Developmental Cycle of Chlamydia trachomatisQ34977705
Protter: interactive protein feature visualization and integration with experimental proteomic dataQ35026816
Site-specific, insertional inactivation of incA in Chlamydia trachomatis using a group II intronQ35082707
Identification of type III secretion substrates of Chlamydia trachomatis using Yersinia enterocolitica as a heterologous systemQ35095777
Efficient inference of recombination hot regions in bacterial genomes.Q35105520
Genomic transcriptional profiling of the developmental cycle of Chlamydia trachomatisQ35168447
Transformation frequency of a mariner-based transposon in Rickettsia rickettsii.Q35192359
A screen of Coxiella burnetii mutants reveals important roles for Dot/Icm effectors and host autophagy in vacuole biogenesisQ35216620
A chemical mutagenesis approach to identify virulence determinants in the obligate intracellular pathogen Chlamydia trachomatisQ35235308
Transposable elementsQ35237512
Chlamydial type III secretion system is encoded on ten operons preceded by sigma 70-like promoter elementsQ35634235
Lateral gene transfer in vitro in the intracellular pathogen Chlamydia trachomatisQ35634466
Hyperactive transposase mutants of the Himar1 mariner transposon.Q35650481
Region-specific insertion of transposons in combination with selection for high plasmid transferability and stability accounts for the structural similarity of IncP-1 plasmidsQ35759677
Chlamydia trachomatis: genome sequence analysis of lymphogranuloma venereum isolatesQ36259301
Interstrain gene transfer in Chlamydia trachomatis in vitro: mechanism and significanceQ36483212
Gene Deletion by Fluorescence-Reported Allelic Exchange Mutagenesis in Chlamydia trachomatisQ36496540
Chlamydia trachomatis plasmid-encoded Pgp4 is a transcriptional regulator of virulence-associated genesQ36646643
Emancipating Chlamydia: Advances in the Genetic Manipulation of a Recalcitrant Intracellular PathogenQ36902486
CD4+ T cells are necessary and sufficient to confer protection against Chlamydia trachomatis infection in the murine upper genital tract.Q36950621
Excision of Tn10 from the donor site during transposition occurs by flush double-strand cleavages at the transposon terminiQ37021318
Transformation and isolation of allelic exchange mutants of Chlamydia psittaci using recombinant DNA introduced by electroporationQ37068255
Characterization of a Coxiella burnetii ftsZ mutant generated by Himar1 transposon mutagenesisQ37110501
Animal models for studying female genital tract infection with Chlamydia trachomatisQ37123840
Characterization of Chlamydia trachomatis plasmid-encoded open reading framesQ37124992
Chlamydia trachomatis diversity viewed as a tissue-specific coevolutionary arms raceQ37384964
Horizontal transfer of tetracycline resistance among Chlamydia spp. in vitroQ37409961
Transposition of fly mariner elements into bacteria as a genetic tool for mutagenesisQ37598102
Tet(C) Gene Transfer between Chlamydia suis Strains Occurs by Homologous Recombination after Co-infection: Implications for Spread of Tetracycline-Resistance among Chlamydiaceae.Q37627187
Expression and targeting of secreted proteins from Chlamydia trachomatis.Q37713167
Chlamydia trachomatis-containing vacuole serves as deubiquitination platform to stabilize Mcl-1 and to interfere with host defense.Q37725770
Summary: The natural history and immunobiology of Chlamydia trachomatis genital infection and implications for Chlamydia controlQ37762768
Hypothetical protein CT398 (CdsZ) interacts with σ(54) (RpoN)-holoenzyme and the type III secretion export apparatus in Chlamydia trachomatisQ37839872
The mouse model of Chlamydia genital tract infection: a review of infection, disease, immunity and vaccine development.Q37845742
Rifampin-resistant RNA polymerase mutants of Chlamydia trachomatis remain susceptible to the ansamycin rifalazilQ37865419
Detailed protocol for purification of Chlamydia pneumoniae elementary bodiesQ37866780
Glucose metabolism in Chlamydia trachomatis: the 'energy parasite' hypothesis revisitedQ37878110
NF-κB control of T cell developmentQ38172394
Potential DNA binding and nuclease functions of ComEC domains characterized in silicoQ38291985
Uptake of extracellular DNA: competence induced pili in natural transformation of Streptococcus pneumoniaeQ39839466
A purified mariner transposase is sufficient to mediate transposition in vitroQ41077576
Genetic elements involved in Tn21 site-specific integration, a novel mechanism for the dissemination of antibiotic resistance genes.Q41207440
Loss of RNase R induces competence development in Legionella pneumophila.Q41365469
Biogenesis of a putative channel protein, ComEC, required for DNA uptake: membrane topology, oligomerization and formation of disulphide bondsQ42113898
RF-Cloning.org: an online tool for the design of restriction-free cloning projects.Q42232925
Natural transformation competence in Helicobacter pylori is mediated by the basic components of a type IV secretion systemQ42656308
Genome sequencing of Chlamydia trachomatis serovars E and F reveals substantial genetic variationQ46251957
Basic charge clusters and predictions of membrane protein topology.Q52037726
Feasibility of a Conditional Knockout System for Chlamydia Based on CRISPR Interference.Q52430804
Floxed-Cassette Allelic Exchange Mutagenesis Enables Markerless Gene Deletion in Chlamydia trachomatis and Can Reverse Cassette-Induced Polar EffectsQ91493267
P433issue4
P921main subjectChlamydia trachomatisQ131065
P577publication date2019-08-06
P1433published inmBioQ15817061
P1476titleTransposon Mutagenesis in Chlamydia trachomatis Identifies CT339 as a ComEC Homolog Important for DNA Uptake and Lateral Gene Transfer
P478volume10

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cites work (P2860)
Q98224831An Ancient Molecular Arms Race: Chlamydia vs. Membrane Attack Complex/Perforin (MACPF) Domain Proteins
Q90007875Bacterial Transformation Buffers Environmental Fluctuations through the Reversible Integration of Mobile Genetic Elements
Q90001380Chromosomal Recombination Targets in Chlamydia Interspecies Lateral Gene Transfer
Q90001369Development of Transposon Mutagenesis for Chlamydia muridarum
Q89741295Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

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