Nanoscale flow cytometry reveals interpatient variability in HIV protease activity that correlates with viral infectivity and identifies drug-resistant viruses

scientific article published on 22 October 2020

Nanoscale flow cytometry reveals interpatient variability in HIV protease activity that correlates with viral infectivity and identifies drug-resistant viruses is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1038/S41598-020-75118-1
P698PubMed publication ID33093566

P2093author name stringMiguel E Quiñones-Mateu
John C Tilton
Aiman A Haqqani
Caroline O Tabler
Jake A Galiatsos
Lauren E Lapointe
Michał M Bonar
Samira Joussef-Piña
P2860cites workTerminal Interface Conformations Modulate Dimer Stability Prior to Amino Terminal Autoprocessing of HIV-1 ProteaseQ27676717
A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening AssaysQ28145712
Contribution of human immunodeficiency virus type 1 minority variants to reduced drug susceptibility in patients on an integrase strand transfer inhibitor-based therapyQ28541754
Identification and characterization of transmitted and early founder virus envelopes in primary HIV-1 infectionQ29619510
Inhibition of autoprocessing of natural variants and multidrug resistant mutant precursors of HIV-1 protease by clinical inhibitorsQ35022049
Sorting of small infectious virus particles by flow virometry reveals distinct infectivity profiles.Q35043212
???Q57904706
High sensitivity detection and sorting of infectious human immunodeficiency virus (HIV-1) particles by flow virometryQ36290494
Partial inhibition of the human immunodeficiency virus type 1 protease results in aberrant virus assembly and the formation of noninfectious particles.Q36650046
Incorporation of Vpr into human immunodeficiency virus type 1 virions: requirement for the p6 region of gag and mutational analysis.Q36655127
Nanoparticle-based flow virometry for the analysis of individual virionsQ37123872
Prototypical Recombinant Multi-Protease-Inhibitor-Resistant Infectious Molecular Clones of Human Immunodeficiency Virus Type 1Q37124113
Antiretroviral Drugs for Treatment and Prevention of HIV Infection in Adults: 2016 Recommendations of the International Antiviral Society-USA PanelQ37234729
Genetic identity, biological phenotype, and evolutionary pathways of transmitted/founder viruses in acute and early HIV-1 infectionQ37273158
Noninvasive high-throughput single-cell analysis of HIV protease activity using ratiometric flow cytometryQ37482762
Visualization of human immunodeficiency virus protease inhibition using a novel Förster resonance energy transfer molecular probeQ38267082
Novel single-cell-level phenotypic assay for residual drug susceptibility and reduced replication capacity of drug-resistant human immunodeficiency virus type 1.Q38449098
Recent Progress in the Development of HIV-1 Protease Inhibitors for the Treatment of HIV/AIDSQ38708006
Quantitative Evaluation of Protein Heterogeneity within Herpes Simplex Virus 1 Particles.Q38919636
Nipah virion entry kinetics, composition, and conformational changes determined by enzymatic virus-like particles and new flow virometry toolsQ38951339
Flexible catalytic site conformations implicated in modulation of HIV-1 protease autoprocessing reactionsQ38972969
Cell-based fluorescence assay for human immunodeficiency virus type 1 protease activityQ39478324
Importance of protease cleavage sites within and flanking human immunodeficiency virus type 1 transframe protein p6* for spatiotemporal regulation of protease activation.Q40005589
Use of a novel assay based on intact recombinant viruses expressing green (EGFP) or red (DsRed2) fluorescent proteins to examine the contribution of pol and env genes to overall HIV-1 replicative fitnessQ40281163
T-cell line for HIV drug screening using EGFP as a quantitative marker of HIV-1 replicationQ40321706
Bis-anthracycline antibiotics inhibit human immunodeficiency virus type 1 transcriptionQ40563544
Initial cleavage of the human immunodeficiency virus type 1 GagPol precursor by its activated protease occurs by an intramolecular mechanismQ41275889
Ordered processing of the human immunodeficiency virus type 1 GagPol precursor is influenced by the context of the embedded viral proteaseQ41997191
Novel method for simultaneous quantification of phenotypic resistance to maturation, protease, reverse transcriptase, and integrase HIV inhibitors based on 3'Gag(p2/p7/p1/p6)/PR/RT/INT-recombinant viruses: a useful tool in the multitarget era of antQ42148219
Quantitative assessment of in vivo HIV protease activity using genetically engineered QD-based FRET probesQ42240414
Western blots versus selected reaction monitoring assays: time to turn the tables?Q42595907
CD4+ T cell kinetics and activation in human immunodeficiency virus-infected patients who remain viremic despite long-term treatment with protease inhibitor-based therapyQ43864378
Single-Particle Discrimination of Retroviruses from Extracellular Vesicles by Nanoscale Flow Cytometry.Q47126636
Misleading Westerns: Common Quantification Mistakes in Western Blot Densitometry and Proposed Corrective MeasuresQ64230716
P433issue1
P921main subjectflow cytometryQ1141429
infectivityQ1662346
P304page(s)18101
P577publication date2020-10-22
P1433published inScientific ReportsQ2261792
P1476titleNanoscale flow cytometry reveals interpatient variability in HIV protease activity that correlates with viral infectivity and identifies drug-resistant viruses
P478volume10

Search more.