Partial purification of the yeast U2 snRNP reveals a novel yeast pre-mRNA splicing factor required for pre-spliceosome assembly

scientific article

Partial purification of the yeast U2 snRNP reveals a novel yeast pre-mRNA splicing factor required for pre-spliceosome assembly is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1093/EMBOJ/18.12.3463
P932PMC publication ID1171425
P698PubMed publication ID10369685
P5875ResearchGate publication ID12928751

P50authorBertrand SéraphinQ28354304
P2093author name stringWilm M
Shevchenko A
Caspary F
P2860cites workSm and Sm-like proteins assemble in two related complexes of deep evolutionary originQ22009995
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis toolsQ24248165
Cloning and domain structure of the mammalian splicing factor U2AFQ24305104
The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formationQ24314710
Phosphorylation of spliceosomal protein SAP 155 coupled with splicing catalysisQ24316022
Correspondence between a mammalian spliceosome component and an essential yeast splicing factorQ24317159
Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex AQ24317598
The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch siteQ24336717
A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch siteQ24522607
Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of Saccharomyces cerevisiae.Q24539048
Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolutionQ24539173
Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114Q24561878
Cleavage and polyadenylation factor CPF specifically interacts with the pre-mRNA 3' processing signal AAUAAAQ24564625
Purification of splicing factor SF1, a heat-stable protein that functions in the assembly of a presplicing complexQ24601157
A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognitionQ24605710
Cloning of cDNAs encoding the 160 kDa subunit of the bovine cleavage and polyadenylation specificity factorQ24626483
A comprehensive set of sequence analysis programs for the VAXQ26778432
Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide GelsQ27860531
Conserved structures and diversity of functions of RNA-binding proteinsQ27861066
Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometryQ27930452
The yeast U2A'/U2B complex is required for pre-spliceosome formationQ27933005
The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP additionQ27933542
A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins.Q27934040
Identification and characterization of a yeast gene encoding the U2 small nuclear ribonucleoprotein particle B" proteinQ27934149
Conservation of structure and subunit interactions in yeast homologues of splicing factor 3b (SF3b) subunitsQ27934190
CUS1, a suppressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145.Q27935463
Cross-intron bridging interactions in the yeast commitment complex are conserved in mammalsQ27936705
Mechanical devices of the spliceosome: motors, clocks, springs, and thingsQ27938081
A link between secretion and pre-mRNA processing defects in Saccharomyces cerevisiae and the identification of a novel splicing gene, RSE1Q27939993
Toward a functional analysis of the yeast genome through exhaustive two-hybrid screensQ28131836
Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding proteinQ28271155
Protein functions in pre-mRNA splicingQ29620262
The structure and function of proteins involved in mammalian pre-mRNA splicingQ29620286
Sm and Sm-like proteins belong to a large family: identification of proteins of the U6 as well as the U1, U2, U4 and U5 snRNPsQ29620439
Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicingQ29620837
Dependence of yeast pre-mRNA 3'-end processing on CFT1: a sequence homolog of the mammalian AAUAAA binding factorQ34063744
Analytical properties of the nanoelectrospray ion sourceQ34394381
repE--the Dictyostelium homolog of the human xeroderma pigmentosum group E gene is developmentally regulated and contains a leucine zipper motifQ34600522
A 127 kDa component of a UV-damaged DNA-binding complex, which is defective in some xeroderma pigmentosum group E patients, is homologous to a slime mold proteinQ34999430
Requirements for U2 snRNP addition to yeast pre-mRNA.Q35925510
Evidence that the 60-kDa protein of 17S U2 small nuclear ribonucleoprotein is immunologically and functionally related to the yeast PRP9 splicing factor and is required for the efficient formation of prespliceosomesQ36518245
Direct interactions between pre-mRNA and six U2 small nuclear ribonucleoproteins during spliceosome assemblyQ36651864
Small nuclear ribonucleoprotein (RNP) U2 contains numerous additional proteins and has a bipartite RNP structure under splicing conditionsQ36659057
Molecular characterization of a novel, widespread nuclear protein that colocalizes with spliceosome componentsQ36849408
Who's on first? The U1 snRNP-5' splice site interaction and splicingQ37281956
Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeastQ37342774
One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteinsQ39717268
RNA splicing. U2 fulfils a commitmentQ40629848
Initial splice-site recognition and pairing during pre-mRNA splicingQ41044111
The yeast branchpoint sequence is not required for the formation of a stable U1 snRNA-pre-mRNA complex and is recognized in the absence of U2 snRNA.Q41080746
Three protein factors (SF1, SF3 and U2AF) function in pre-splicing complex formation in addition to snRNPsQ41081059
A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5' cleavage site.Q41107145
Drosophila SNF/D25 combines the functions of the two snRNP proteins U1A and U2B' that are encoded separately in human, potato, and yeastQ41907964
An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathwayQ44540184
Efficient association of U2 snRNPs with pre-mRNA requires an essential U2 RNA structural elementQ45351813
In vitro assembly of yeast U6 snRNP: a functional assayQ46978210
New constructs and strategies for efficient PCR-based gene manipulations in yeast.Q52532237
Protein components specifically associated with prespliceosome and spliceosome complexesQ54261211
Comparative analysis of binding of human damaged DNA-binding protein (XPE) and Escherichia coli damage recognition protein (UvrA) to the major ultraviolet photoproducts: T[c,s]T, T[t,s]T, T[6-4]T, and T[Dewar]T.Q54649893
Presplicing complex formation requires two proteins and U2 snRNPQ67970038
Mutational analysis of the interactions between U1 small nuclear RNA and pre-mRNA of yeastQ69554872
mRNA splicing efficiency in yeast and the contribution of nonconserved sequencesQ69891962
Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNAQ70495958
Interaction of mammalian splicing factor SF3a with U2 snRNP and relation of its 60-kD subunit to yeast PRP9Q72535904
Interaction between PRP11 and SPP91 yeast splicing factors and characterization of a PRP9-PRP11-SPP91 complexQ72535907
Parameters affecting lithium acetate-mediated transformation of Saccharomyces cerevisiae and development of a rapid and simplified procedureQ72752191
Separation of splicing factor SF3 into two components and purification of SF3a activityQ72875831
A shortcut to interesting human genes: peptide sequence tags, expressed-sequence tags and computersQ73015810
P433issue12
P407language of work or nameEnglishQ1860
P921main subjectspliceosomeQ915868
U2 snRNP complex subunit RSE1 YML049CQ27549663
P304page(s)3463-3474
P577publication date1999-06-01
P1433published inThe EMBO JournalQ1278554
P1476titlePartial purification of the yeast U2 snRNP reveals a novel yeast pre-mRNA splicing factor required for pre-spliceosome assembly
P478volume18

Reverse relations

cites work (P2860)
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