An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments

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An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments is …
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scholarly articleQ13442814

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P356DOI10.1529/BIOPHYSJ.107.117689
P3181OpenCitations bibliographic resource ID1511513
P932PMC publication ID2242739
P698PubMed publication ID17921203
P5875ResearchGate publication ID5924267

P50authorColin Echeverría AitkenQ42130282
P2093author name stringJoseph D Puglisi
R Andrew Marshall
P2860cites workSingle-molecule enzymologyQ28144763
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Biochemistry without oxygenQ69010813
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Studies on Ascorbic Acid. II. Physical Changes in Catalase Following Incubation with Ascorbate or Ascorbate and Copper(II)*Q72305152
The use of protocatechuate dioxygenase for maintaining anaerobic conditions in biochemical experimentsQ73162677
Nonblinking and long-lasting single-molecule fluorescence imagingQ79168298
Blinking fluorophores: what do they tell us about protein dynamics?Q79276393
Direct observation of delayed fluorescence from a remarkable back-isomerization in Cy5Q81801076
DITHIOTHREITOL, A NEW PROTECTIVE REAGENT FOR SH GROUPSQ28198197
Reactive species formed on proteins exposed to singlet oxygenQ28240610
Strategies to improve photostabilities in ultrasensitive fluorescence spectroscopyQ28283684
tRNA dynamics on the ribosome during translationQ30832302
Fluorescence spectroscopy of single biomolecules.Q31221311
Spectral identification of specific photophysics of cy5 by means of ensemble and single molecule measurementsQ33230879
Analysis of photobleaching in single-molecule multicolor excitation and Förster resonance energy transfer measurementsQ33235128
Singlet oxygen and dye-triplet-state quenching in solid-state dye lasers consisting of pyrromethene 567-doped poly(methyl methacrylate).Q33322828
Combining optical trapping and single-molecule fluorescence spectroscopy: enhanced photobleaching of fluorophoresQ33379198
Force measurements by micromanipulation of a single actin filament by glass needlesQ34177181
Identification of an antioxidant, ethyl protocatechuate, in peanut seed testaQ34187534
Real-time observation of RecA filament dynamics with single monomer resolution.Q34556173
Single-molecule foldingQ35064536
Singlet oxygen induced DNA damageQ35341253
Measuring the folding transition time of single RNA moleculesQ35751455
Biophysical analyses of designed and selected mutants of protocatechuate 3,4-dioxygenase1.Q35919875
Single-molecule fluorescence spectroscopy of protein folding.Q36180888
Fluctuating enzymes: lessons from single-molecule studies.Q36344041
Rigidity of microtubules is increased by stabilizing agentsQ36382638
Identification of two distinct hybrid state intermediates on the ribosomeQ36462728
Single-molecule analysis of RNA polymerase transcriptionQ36475400
Purification and properties of protocatechuate 3,4-dioxygenase from Pseudomonas putida. A new iron to subunit stoichiometryQ36596004
Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallateQ36648121
A survey of single-molecule techniques in chemical biologyQ36715680
Comparison of anti-fading agents used in fluorescence microscopy: image analysis and laser confocal microscopy studyQ36756292
Analysis of antifading reagents for fluorescence microscopyQ39380328
A comparison between the sulfhydryl reductants tris(2-carboxyethyl)phosphine and dithiothreitol for use in protein biochemistryQ41685858
Studies on Ascorbic Acid. I. Factors Influencing the Ascorbate-Mediated Inhibition of Catalase*Q44099933
Initiation and re-initiation of DNA unwinding by the Escherichia coli Rep helicaseQ44175920
Spectroscopic study and evaluation of red-absorbing fluorescent dyesQ44277253
Hemoglobin-oxygen equilibrium curves measured during enzymatic oxygen consumptionQ44842762
tRNA selection and kinetic proofreading in translationQ45078350
Carbocyanine dyes as efficient reversible single-molecule optical switchQ46383352
Photodestruction intermediates probed by an adjacent reporter molecule.Q50351257
Antifading agents for confocal fluorescence microscopy.Q52573393
Single-Molecule Measurement of Protein Folding KineticsQ59332599
P433issue5
P407language of work or nameEnglishQ1860
P921main subjectoxygenQ629
biophysicsQ7100
P1104number of pages10
P304page(s)1826-1835
P577publication date2007-10-05
P1433published inBiophysical JournalQ2032955
P1476titleAn oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments
P478volume94

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Q50009840Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions.
Q34412426VASP is a processive actin polymerase that requires monomeric actin for barbed end association
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