Use of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin-Microtubule Complexes

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Use of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin-Microtubule Complexes is …
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scholarly articleQ13442814

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P356DOI10.1007/978-1-4939-7271-5_11
P932PMC publication ID5760212
P698PubMed publication ID28940071

P2093author name stringHernando Sosa
Matthieu P M H Benoit
P2860cites workPreparation of microtubule protein and purified tubulin from bovine brain by cycles of assembly and disassembly and phosphocellulose chromatographyQ37751160
Processive cytoskeletal motors studied with single-molecule fluorescence techniques.Q38216570
Fluorescence polarization microscopyQ38611092
ADP-induced rocking of the kinesin motor domain revealed by single-molecule fluorescence polarization microscopyQ43617704
Three-dimensional structural dynamics of myosin V by single-molecule fluorescence polarizationQ44379280
Configuration of the two kinesin motor domains during ATP hydrolysisQ44582779
Nucleotide binding and hydrolysis induces a disorder-order transition in the kinesin neck-linker regionQ79749645
An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experimentsQ24647395
Polarized fluorescence microscopy of individual and many kinesin motors bound to axonemal microtubulesQ28362015
Dynamic measurement of myosin light-chain-domain tilt and twist in muscle contraction.Q30322844
Myosin conformational states determined by single fluorophore polarizationQ32057541
Assays for kinesin microtubule-stimulated ATPase activityQ32057726
Kinesin moving through the spotlight: single-motor fluorescence microscopy with submillisecond time resolution.Q33269738
Structure and dynamics of the kinesin-microtubule interaction revealed by fluorescence polarization microscopyQ33577618
Future challenges in single-molecule fluorescence and laser trap approaches to studies of molecular motorsQ34511756
Stepping rotation of F1-ATPase visualized through angle-resolved single-fluorophore imagingQ35155735
Single-molecule fluorescence spectroscopy and microscopy of biomolecular motors.Q35760214
Distinct Interaction Modes of the Kinesin-13 Motor Domain with the MicrotubuleQ36804148
Elongation factor G initiates translocation through a power strokeQ37086449
A mobile kinesin-head intermediate during the ATP-waiting stateQ37153671
P407language of work or nameEnglishQ1860
P304page(s)199-216
P577publication date2018-01-01
P1433published inMethods in Molecular BiologyQ15752859
P1476titleUse of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin-Microtubule Complexes
P478volume1665

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Q90009145Fluorescence microscopy applied to intracellular transport by microtubule motorscites workP2860

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