In vitro characterization of a recombinant Blh protein from an uncultured marine bacterium as a beta-carotene 15,15'-dioxygenase

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In vitro characterization of a recombinant Blh protein from an uncultured marine bacterium as a beta-carotene 15,15'-dioxygenase is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1074/JBC.M109.002618
P932PMC publication ID2708875
P698PubMed publication ID19366683

P50authorSoo-Jin YeomQ59194041
P2093author name stringYeong-Su Kim
Seon-Won Kim
Deok-Kun Oh
Nam-Hee Kim
P2860cites workGenome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead SeaQ22065753
Genome sequence of Halobacterium species NRC-1Q22066243
The genome of Salinibacter ruber: convergence and gene exchange among hyperhalophilic bacteria and archaeaQ22066350
Evolution in the laboratory: The genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1Q22162489
Cloning and characterization of a human beta,beta-carotene-15,15'-dioxygenase that is highly expressed in the retinal pigment epitheliumQ24291318
Proteorhodopsin photosystem gene expression enables photophosphorylation in a heterologous hostQ24674568
CHARMM: A program for macromolecular energy, minimization, and dynamics calculationsQ53340989
Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study.Q55048769
Stoichiometric Conversion ofall trans-β-Carotene to Retinal by Pig Intestinal ExtractQ60193569
In vitro and in vivo inhibition of beta-carotene dioxygenase activity by canthaxanthin in rat intestineQ74063263
The conversion of beta-carotene into vitamin AQ78850542
Effective production of retinal from beta-carotene using recombinant mouse beta-carotene 15,15'-monooxygenaseQ80769409
The Reaction Mechanism of the Enzyme-Catalyzed Central Cleavage of beta-Carotene to Retinal This research was supported by F. Hoffmann-La Roche AG and the Swiss National Science Foundation. We are grateful to F. Hoffmann-La Roche AG for a generous gQ95822678
New insights into metabolic properties of marine bacteria encoding proteorhodopsinsQ24811174
PROCHECK: a program to check the stereochemical quality of protein structuresQ26778411
Protein secondary structure prediction based on position-specific scoring matricesQ27860483
Predicting transmembrane protein topology with a hidden Markov model: application to complete genomesQ27860838
Comparative protein modelling by satisfaction of spatial restraintsQ27860866
The structure of a retinal-forming carotenoid oxygenaseQ28244367
Mechanism of the Biosynthesis of Vitamin A from β-CaroteneQ28257698
Expression pattern and localization of beta,beta-carotene 15,15'-dioxygenase in different tissuesQ28359602
Identification, expression, and substrate specificity of a mammalian beta-carotene 15,15'-dioxygenaseQ28592200
Cloning and expression of beta,beta-carotene 15,15'-dioxygenaseQ28592296
Provitamin A conversion to retinal via the beta,beta-carotene-15,15'-oxygenase (bcox) is essential for pattern formation and differentiation during zebrafish embryogenesisQ28645784
Prediction of protein side-chain rotamers from a backbone-dependent rotamer library: a new homology modeling toolQ30427274
Molecular analysis of vitamin A formation: cloning and characterization of beta-carotene 15,15'-dioxygenasesQ30652323
Expression and characterization of a murine enzyme able to cleave beta-carotene. The formation of retinoidsQ30679980
Cloning, expression and characterization of a functional cDNA clone encoding geranylgeranyl diphosphate synthase of Hevea brasiliensisQ30881651
Analogous enzymes: independent inventions in enzyme evolutionQ32028978
Filling the gap in vitamin A research. Molecular identification of an enzyme cleaving beta-carotene to retinalQ33898267
Codon bias and heterologous protein expressionQ33979754
Bacterial phytoene synthase: molecular cloning, expression, and characterization of Erwinia herbicola phytoene synthaseQ34184128
Proteorhodopsin photosystem gene clusters exhibit co-evolutionary trends and shared ancestry among diverse marine microbial phylaQ34577632
Intestinal absorption and metabolism of carotenoids: insights from cell cultureQ35869697
Isolation, sequence, and characterization of the Cercospora nicotianae phytoene dehydrogenase gene.Q36057556
bop gene cluster expression in bacteriorhodopsin-overproducing mutants of Halobacterium halobiumQ36106002
The enzymatic cleavage of beta-carotene into vitamin A by soluble enzymes of rat liver and intestineQ36380605
Genetic and topological analyses of the bop promoter of Halobacterium halobium: stimulation by DNA supercoiling and non-B-DNA structureQ39840175
The Enzymatic Cleavage of β-Carotene: Still ControversialQ40477825
Identification of beta-carotene 15, 15'-monooxygenase as a peroxisome proliferator-activated receptor target geneQ40646216
Biochemical properties of purified recombinant human beta-carotene 15,15'-monooxygenaseQ42051753
A new isopentenyl diphosphate isomerase gene from Camptotheca acuminata: cloning, characterization and functional expression in Escherichia coliQ42646276
Molecular cloning, characterization, and differential expression of a farnesyl-diphosphate synthase gene from the basidiomycetous fungus Ganoderma lucidumQ42658469
Vitamin A and carotenoids. The enzymic conversion of β-carotene into retinal in hog intestinal mucosaQ42934436
brp and blh are required for synthesis of the retinal cofactor of bacteriorhodopsin in Halobacterium salinarumQ43510596
Key role of conserved histidines in recombinant mouse beta-carotene 15,15'-monooxygenase-1 activityQ46542183
In vitro characterization of a carotenoid cleavage dioxygenase from Nostoc sp. PCC 7120 reveals a novel cleavage pattern, cytosolic localization and induction by highlightQ46591806
Isolation and characterization of a stress-inducible Dunaliella salina Lcy-beta gene encoding a functional lycopene beta-cyclaseQ46608799
Purification and properties of carotene 15,15'-dioxygenase of rabbit intestineQ46616280
Reaction mechanism of apocarotenoid oxygenase (ACO): a DFT study.Q46818368
Assay of beta-carotene 15,15'-dioxygenase activity by reverse-phase high-pressure liquid chromatographyQ48889363
The Reaction Mechanism of the Enzyme-Catalyzed Central Cleavage of β-Carotene to Retinal.Q52715042
P433issue23
P407language of work or nameEnglishQ1860
P921main subjectcell biologyQ7141
P304page(s)15781-15793
P577publication date2009-04-14
P1433published inJournal of Biological ChemistryQ867727
P1476titleIn vitro characterization of a recombinant Blh protein from an uncultured marine bacterium as a beta-carotene 15,15'-dioxygenase
P478volume284

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cites work (P2860)
Q42717408Characterization of an apo-carotenoid 13,14-dioxygenase from Novosphingobium aromaticivorans that converts β-apo-8'-carotenal to β-apo-13-carotenone
Q64113933Formation and Cleavage of C–C Bonds by Enzymatic Oxidation–Reduction Reactions
Q33946099Metagenomic identification of a novel salt tolerance gene from the human gut microbiome which encodes a membrane protein with homology to a brp/blh-family β-carotene 15,15'-monooxygenase.
Q24339437Production of β-apo-10'-carotenal from β-carotene by human β-carotene-9',10'-oxygenase expressed in E. coli
Q36048640Purification and characterization of a novel β-carotene-9',10'-oxygenase from Saccharomyces cerevisiae ULI3.
Q48095607Solar-panel and parasol strategies shape the proteorhodopsin distribution pattern in marine Flavobacteriia
Q58103908Species widely distributed in halophilic Archaea exhibit opsin-mediated inhibition of bacterioruberin biosynthesis
Q33676794The human enzyme that converts dietary provitamin A carotenoids to vitamin A is a dioxygenase
Q57470158acI Actinobacteria Assemble a Functional Actinorhodopsin with Natively-synthesized Retinal