Enrichment techniques employed in phosphoproteomics

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Enrichment techniques employed in phosphoproteomics is …
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scholarly articleQ13442814
review articleQ7318358

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P6179Dimensions Publication ID1017728901
P356DOI10.1007/S00726-011-1111-Z
P3181OpenCitations bibliographic resource ID964037
P932PMC publication ID3418503
P698PubMed publication ID22002794
P5875ResearchGate publication ID51719770

P2093author name stringDavid Honys
Jan Fíla
P2860cites workPrecipitation of calcium phosphate in the histochemical method for phosphataseQ73101535
Improved immobilized metal affinity chromatography for large-scale phosphoproteomics applicationsQ79192660
Optimization of phosphopeptide elution conditions in immobilized Fe(III) affinity chromatographyQ79412653
Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometryQ79491768
Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applicationsQ79830472
Immobilized zirconium ion affinity chromatography for specific enrichment of phosphopeptides in phosphoproteome analysisQ80492077
A quantitative investigation into the losses of proteins at different stages of a two-dimensional gel electrophoresis procedureQ81868524
Analysis of desiccation-induced candidate phosphoproteins from Craterostigma plantagineum isolated with a modified metal oxide affinity chromatography procedureQ81887144
Selective zirconium dioxide-based enrichment of phosphorylated peptides for mass spectrometric analysisQ82822194
Hydroxyapatite affinity chromatography for the highly selective enrichment of mono- and multi-phosphorylated peptides in phosphoproteome analysisQ84970306
Immunoaffinity profiling of tyrosine phosphorylation in cancer cellsQ21735929
Large-scale characterization of HeLa cell nuclear phosphoproteinsQ24300834
Phosphoproteome analysis of the human mitotic spindleQ24547339
A quantitative atlas of mitotic phosphorylationQ24646817
Analysis of receptor signaling pathways by mass spectrometry: identification of vav-2 as a substrate of the epidermal and platelet-derived growth factor receptorsQ24649799
Combining metal oxide affinity chromatography (MOAC) and selective mass spectrometry for robust identification of in vivo protein phosphorylation sitesQ24812383
Global, in vivo, and site-specific phosphorylation dynamics in signaling networksQ27864128
The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SWI5.Q27932153
A mass spectrometry-based proteomic approach for identification of serine/threonine-phosphorylated proteins by enrichment with phospho-specific antibodies: identification of a novel protein, Frigg, as a protein kinase A substrateQ28202635
Proteomics by mass spectrometry: approaches, advances, and applicationsQ28243072
Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometryQ28266730
Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compoundsQ28281478
Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiaeQ29618445
Enrichment analysis of phosphorylated proteins as a tool for probing the phosphoproteomeQ30660384
Immobilized gallium(III) affinity chromatography of phosphopeptidesQ30744823
Factors governing the solubilization of phosphopeptides retained on ferric NTA IMAC beads and their analysis by MALDI TOFMS.Q30855809
Global quantitative phosphoprotein analysis using Multiplexed Proteomics technologyQ30966244
Susceptibility of the hydroxyl groups in serine and threonine to β-elimination/Michael addition under commonly used moderately high-temperature conditionsQ31026703
Phosphoric acid enhances the performance of Fe(III) affinity chromatography and matrix-assisted laser desorption/ionization tandem mass spectrometry for recovery, detection and sequencing of phosphopeptidesQ31102375
Combined enzymatic and data mining approaches for comprehensive phosphoproteome analyses: application to cell signaling events of interferon-gamma-stimulated macrophagesQ31136566
Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns.Q31164900
Titania as a chemo-affinity support for the column-switching HPLC analysis of phosphopeptides: application to the characterization of phosphorylation sites in proteins by combination with protease digestion and electrospray ionization mass spectromeQ33203332
Specificity of immobilized metal affinity-based IMAC/C18 tip enrichment of phosphopeptides for protein phosphorylation analysisQ33221653
Enhanced phosphopeptide isolation by Fe(III)-IMAC using 1,1,1,3,3,3-hexafluoroisopropanolQ33227434
Use of detergents to increase selectivity of immunoprecipitation of tyrosine phosphorylated peptides prior to identification by MALDI quadrupole-TOF MS.Q33229116
Comprehensive identification of phosphorylation sites in postsynaptic density preparationsQ33232861
Phosphopeptide modification and enrichment by oxidation-reduction condensationQ33267367
Highly specific enrichment of phosphopeptides by zirconium dioxide nanoparticles for phosphoproteome analysis.Q33286289
Highly Efficient Phosphopeptide Enrichment by Calcium Phosphate Precipitation Combined with Subsequent IMAC EnrichmentQ33293056
Development of a titanium dioxide nanoparticle pipette-tip for the selective enrichment of phosphorylated peptidesQ33294937
Coupling of TiO(2)-mediated enrichment and on-bead guanidinoethanethiol labeling for effective phosphopeptide analysis by matrix-assisted laser desorption/ionization mass spectrometryQ33305456
SIMAC (sequential elution from IMAC), a phosphoproteomics strategy for the rapid separation of monophosphorylated from multiply phosphorylated peptidesQ33307225
The rate of nuclear cytoplasmic protein transport is determined by the casein kinase II site flanking the nuclear localization sequence of the SV40 T-antigenQ33334884
Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysisQ33352234
Enrichment of phosphoproteins and phosphopeptide derivatization identify universal stress proteins in elicitor-treated ArabidopsisQ33368793
Enrichment and analysis of phosphopeptides under different experimental conditions using titanium dioxide affinity chromatography and mass spectrometryQ33518768
In-depth analyses of kinase-dependent tyrosine phosphoproteomes based on metal ion-functionalized soluble nanopolymersQ33609440
Hydroxyapatite as a concentrating probe for phosphoproteomic analyses.Q33681634
Phosphorylation of the yeast ribosomal stalk. Functional effects and enzymes involved in the processQ33753454
Mitochondrial phosphoproteome revealed by an improved IMAC method and MS/MS/MS.Q33989191
Signaling through MAP kinase networks in plantsQ33997191
Comparison of metal and metal oxide media for phosphopeptide enrichment prior to mass spectrometric analysesQ34315142
Discontinuous pH gradient-mediated separation of TiO2-enriched phosphopeptidesQ34409726
Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column.Q34734854
Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatographyQ34758135
Large-scale phosphorylation analysis of mouse liverQ35616333
Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry.Q35629586
Evidence for phosphorylation of serine 753 in CFTR using a novel metal-ion affinity resin and matrix-assisted laser desorption mass spectrometryQ36280213
Improved enrichment strategies for phosphorylated peptides on titanium dioxide using methyl esterification and pH gradient elutionQ36777859
Optimizing TiO2-based phosphopeptide enrichment for automated multidimensional liquid chromatography coupled to tandem mass spectrometry.Q36873470
Phosphoproteomic analysis of human brain by calcium phosphate precipitation and mass spectrometryQ37146736
Phosphopeptide fragmentation and analysis by mass spectrometryQ37510777
Phosphorylation of Isocitrate Dehydrogenase of Escherichia coliQ39240952
Immobilized metal affinity chromatography revisited: pH/acid control toward high selectivity in phosphoproteomicsQ39950119
Immunoaffinity enrichments followed by mass spectrometric detection for studying global protein tyrosine phosphorylation.Q39973528
Global profiling of phosphopeptides by titania affinity enrichmentQ40069163
Enrichment of phosphorylated proteins from cell lysate using a novel phosphate-affinity chromatography at physiological pH.Q40238118
Efficient enrichment of intact phosphorylated proteins by modified immobilized metal-affinity chromatography.Q40357569
Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site databaseQ40489678
The substrate specificity and inhibition of alkaline phosphatases of cow's milk and calf intestinal mucosaQ41767014
Reaction of phosphorylated and O-glycosylated peptides by chemically targeted identification at ambient temperatureQ42073588
Tyrosine phosphorylation of RNA polymerase II carboxyl-terminal domain by the Abl-related gene product.Q42441560
Purification of phosphoproteins by immobilized metal affinity chromatography and its application to phosphoproteome analysisQ42828634
Optimized IMAC-IMAC protocol for phosphopeptide recovery from complex biological samplesQ43070131
The phosphoproteome of Fusarium graminearum at the onset of nitrogen starvation.Q43245934
Effect of peptide-to-TiO2 beads ratio on phosphopeptide enrichment selectivityQ43275362
A survey of the Arabidopsis thaliana mitochondrial phosphoproteomeQ43290333
A new acid mix enhances phosphopeptide enrichment on titanium- and zirconium dioxide for mapping of phosphorylation sites on protein complexesQ43469140
A systematic approach to the analysis of protein phosphorylationQ43563012
Subproteomics analysis of phosphorylated proteins: application to the study of B-lymphoblasts from a patient with Scott syndromeQ44068979
A new approach to phosphoserine and phosphothreonine analysis in peptides and proteins: chemical modification, enrichment via solid-phase reversible binding, and analysis by mass spectrometryQ44432267
Successive and selective release of phosphorylated peptides captured by hydroxy acid-modified metal oxide chromatographyQ44645118
Proteomic analysis of in vivo phosphorylated synaptic proteins.Q45168689
Quantitative phosphoproteomics applied to the yeast pheromone signaling pathwayQ45234630
Reduction of non-specific binding in Ga(III) immobilized metal affinity chromatography for phosphopeptides by using endoproteinase glu-C as the digestive enzymeQ45244395
Novel immobilized zinc(II) affinity chromatography for phosphopeptides and phosphorylated proteinsQ45309919
Nucleic acids in protein samples interfere with phosphopeptide identification by immobilized-metal-ion affinity chromatography and mass spectrometry.Q46027042
Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichmentQ46050662
Increasing phosphoproteome coverage and identification of phosphorylation motifs through combination of different HPLC fractionation methodsQ46431514
Actin depolymerizing factor is essential for viability in plants, and its phosphoregulation is important for tip growth.Q46734827
Enrichment of phosphorylated proteins and peptides from complex mixtures using metal oxide/hydroxide affinity chromatography (MOAC).Q46750738
Hydrophilic interaction chromatography reduces the complexity of the phosphoproteome and improves global phosphopeptide isolation and detectionQ46796552
Development of mesoporous TiO(2) microspheres with high specific surface area for selective enrichment of phosphopeptides by mass spectrometric analysisQ48277232
Improved method of phosphopeptides enrichment using biphasic phosphate-binding tag/C18 tip for versatile analysis of phosphorylation dynamicsQ48432077
Plant protein phosphorylation monitored by capillary liquid chromatography--element mass spectrometry.Q50697956
Comprehensive profiling of phosphopeptides based on anion exchange followed by flow-through enrichment with titanium dioxide (AFET).Q53071888
Quantitative analysis of a multicomponent analgesic product containing butalbital, using high speed reverse-phase liquid chromatography.Q54372887
Proteome mapping of mature pollen of Arabidopsis thaliana.Q54634229
Reproducible isolation of distinct, overlapping segments of the phosphoproteomeQ57205837
Evaluation of the impact of some experimental procedures on different phosphopeptide enrichment techniquesQ57279153
Selective Isolation at the Femtomole Level of Phosphopeptides from Proteolytic Digests Using 2D-NanoLC-ESI-MS/MS and Titanium Oxide PrecolumnsQ57666311
Titanium dioxide as a chemo-affinity solid phase in offline phosphopeptide chromatography prior to HPLC-MS/MS analysisQ57887340
Fractionation of whole casein on hydroxyapatite. Application to a study of buffalo kappa-caseinQ67568264
Isolation of phosphoproteins by immobilized metal (Fe3+) affinity chromatographyQ70287638
P433issue3
P407language of work or nameEnglishQ1860
P921main subjectpeptideQ172847
phosphoproteinQ423934
proteomeQ860947
plant proteinsQ74708517
P304page(s)1025-47
P577publication date2012-09-01
P1433published inAmino AcidsQ15757015
P1476titleEnrichment techniques employed in phosphoproteomics
P478volume43

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cites work (P2860)
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