Proteolytic Digestion and TiO2 Phosphopeptide Enrichment Microreactor for Fast MS Identification of Proteins

scientific article published on 16 February 2016

Proteolytic Digestion and TiO2 Phosphopeptide Enrichment Microreactor for Fast MS Identification of Proteins is …
instance of (P31):
scholarly articleQ13442814

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P6179Dimensions Publication ID1006302619
P356DOI10.1007/S13361-015-1332-6
P698PubMed publication ID26883530

P50authorIulia M. LazarQ37839393
P2093author name stringJingren Deng
P2860cites workExploring the human leukocyte phosphoproteome using a microfluidic reversed-phase-TiO2-reversed-phase high-performance liquid chromatography phosphochip coupled to a quadrupole time-of-flight mass spectrometerQ39754465
Chip-Based Enrichment and NanoLC-MS/MS Analysis of Phosphopeptides from Whole Lysates.Q40007888
Proteome profile of the MCF7 cancer cell line: a mass spectrometric evaluationQ40229711
Mass spectrometric study of the effects of hydrophobic surface chemistry and morphology on the digestion of surface-bound proteinsQ44369988
Integrated sample preparation methodology for proteomics: analysis of native proteinsQ47797164
Efficient protein digestion with peptide separation in a micro-device interfaced to electrospray mass spectrometry.Q51220090
Titania and alumina sol-gel-derived microfluidics enzymatic-reactors for peptide mapping: design, characterization, and performance.Q51568020
Controlled proteolysis of normal and pathological prion protein in a microfluidic chip.Q51575758
On-chip proteolytic digestion and analysis using "wrong-way-round" electrospray time-of-flight mass spectrometry.Q51603292
High-Speed TOFMS Detection for Capillary ElectrophoresisQ60976853
Microwave-assisted reactions in organic synthesis--are there any nonthermal microwave effects?Q84553581
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A microchip-based proteolytic digestion system driven by electroosmotic pumpingQ30312173
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Nanopore-based proteolytic reactor for sensitive and comprehensive proteomic analysesQ30819581
Microwave-enhanced enzyme reaction for protein mapping by mass spectrometry: a new approach to protein digestion in minutesQ30863794
Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns.Q31164900
Microfluidic enzymatic-reactors for peptide mapping: strategy, characterization, and performanceQ33209423
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Highly efficient enrichment and subsequent digestion of proteins in the mesoporous molecular sieve silicate SBA-15 for matrix-assisted laser desorption/ionization mass spectrometry with time-of-flight/time-of-flight analyzer peptide mappingQ33258134
Gold nanoparticle assembly microfluidic reactor for efficient on-line proteolysisQ33285458
Enhanced protein digestion through the confinement of nanozeolite-assembled microchip reactorsQ33322595
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Digestion of native proteins for proteomics using a thermocycler.Q33346570
Accelerated proteolysis in alternating electric fields for peptide mappingQ33370600
Highly efficient enzyme reactors containing trypsin and endoproteinase LysC immobilized on porous polymer monolith coupled to MS suitable for analysis of antibodiesQ33404826
Proteomic snapshot of breast cancer cell cycle: G1/S transition point.Q34010320
Nanobiocatalysis for protein digestion in proteomic analysisQ34011515
Protein digestion: an overview of the available techniques and recent developmentsQ34569268
Online nanoflow multidimensional fractionation for high efficiency phosphopeptide analysisQ35579649
Rapid and reproducible single-stage phosphopeptide enrichment of complex peptide mixtures: application to general and phosphotyrosine-specific phosphoproteomics experimentsQ35650675
Optimizing TiO2-based phosphopeptide enrichment for automated multidimensional liquid chromatography coupled to tandem mass spectrometry.Q36873470
Effects of modified digestion schemes on the identification of proteins from complex mixturesQ36874088
Application of pressurized solvents for ultrafast trypsin hydrolysis in proteomics: proteomics on the fly.Q37347394
Techniques for phosphopeptide enrichment prior to analysis by mass spectrometry.Q37407214
Overview on modern approaches to speed up protein identification workflows relying on enzymatic cleavage and mass spectrometry-based techniquesQ37589829
Enzyme-immobilized microfluidic process reactors.Q37902865
P433issue4
P304page(s)686-698
P577publication date2016-02-16
P1433published inJournal of the American Society for Mass SpectrometryQ3020250
P1476titleProteolytic Digestion and TiO2 Phosphopeptide Enrichment Microreactor for Fast MS Identification of Proteins
P478volume27

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cites work (P2860)
Q91449517Streamlined microfluidic analysis of phosphopeptides using stable isotope-labeled synthetic peptides and MRM-MS detection
Q30274857Structural, thermodynamic, and phosphatidylinositol 3-phosphate binding properties of Phafin2.

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