Structural and thermodynamic analysis of the GFP:GFP-nanobody complex

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Structural and thermodynamic analysis of the GFP:GFP-nanobody complex is …
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scholarly articleQ13442814

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P356DOI10.1002/PRO.519
P3181OpenCitations bibliographic resource ID1080938
P932PMC publication ID3009406
P698PubMed publication ID20945358
P5875ResearchGate publication ID47416165

P50authorKirill AlexandrovQ43181060
Oleksiy KovtunQ56986774
Brett M. CollinsQ37838767
P2093author name stringMarta H Kubala
P2860cites workConstructing and exploiting the fluorescent protein paintbox (Nobel Lecture).Q45957909
Camelid immunoglobulins and nanobody technology.Q47221693
Protein mislocalization in plant cells using a GFP-binding chromobodyQ50585437
Identification of a universal VHH framework to graft non-canonical antigen-binding loops of camel single-domain antibodies.Q54480911
Strongin VivoMaturation Compensates for Structurally Restricted H3 Loops in Antibody RepertoiresQ57985824
Heat capacity effects in protein folding and ligand binding: a re-evaluation of the role of water in biomolecular thermodynamicsQ81487970
Hydration of protein-protein interfacesQ81692270
A single-domain antibody fragment in complex with RNase A: non-canonical loop structures and nanomolar affinity using two CDR loopsQ27617837
Degenerate interfaces in antigen-antibody complexesQ27635520
Antibody multispecificity mediated by conformational diversityQ27640592
Crystal structure of the intrinsically flexible addiction antidote MazEQ27641242
Evidence that structural rearrangements and/or flexibility during TCR binding can contribute to T cell activationQ27642839
Toward chaperone-assisted crystallography: Protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffoldQ27650478
Nanobody-aided structure determination of the EpsI:EpsJ pseudopilin heterodimer from Vibrio vulnificusQ27653312
Crystal Structure of the N-Terminal Domain of the Secretin GspD from ETEC Determined with the Assistance of a NanobodyQ27653797
Modulation of protein properties in living cells using nanobodiesQ27658661
Crystal structure of the Aequorea victoria green fluorescent proteinQ27733214
Coot: model-building tools for molecular graphicsQ27860505
The finer things in X-ray diffraction data collectionQ27860660
Developments in the CCP4 molecular-graphics projectQ27860702
Phasercrystallographic softwareQ27860930
PHENIX: building new software for automated crystallographic structure determinationQ27861044
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent proteinQ27861056
A versatile nanotrap for biochemical and functional studies with fluorescent fusion proteinsQ28254176
Targeting and tracing antigens in live cells with fluorescent nanobodiesQ28270182
Alternative non-antibody scaffolds for molecular recognitionQ33291380
Species-independent translational leaders facilitate cell-free expressionQ33489431
Molecular basis for the preferential cleft recognition by dromedary heavy-chain antibodiesQ34574853
Protein-protein interactions: mechanisms and modification by drugsQ35034125
Thermodynamics of protein-ligand interactions: history, presence, and future aspectsQ35877480
Heat capacity in proteinsQ36083067
Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transferQ36804073
Survey of the year 2008: applications of isothermal titration calorimetryQ37705184
Protein-protein interfaces: architectures and interactions in protein-protein interfaces and in protein cores. Their similarities and differencesQ41063777
Protein binding versus protein folding: the role of hydrophilic bridges in protein associationsQ41313934
P433issue12
P304page(s)2389-2401
P577publication date2010-12-01
P1433published inProtein ScienceQ7251445
P1476titleStructural and thermodynamic analysis of the GFP:GFP-nanobody complex
P478volume19

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cites work (P2860)
Q37544893A Nanobody Activation Immunotherapeutic that Selectively Destroys HER2-Positive Breast Cancer Cells
Q47302135A Split-Luciferase Reporter Recognizing GFP and mCherry Tags to Facilitate Studies of Protein-Protein Interactions.
Q28603328A nanobody:GFP bacterial platform that enables functional enzyme display and easy quantification of display capacity
Q50464467A new arene-Ru based supramolecular coordination complex for efficient binding and selective sensing of green fluorescent protein.
Q24328817A single PXXP motif in the C-terminal region of srGAP3 mediates binding to multiple SH3 domains
Q56775209A versatile nanobody-based toolkit to analyze retrograde transport from the cell surface
Q52898287Affinity enhancement of nanobody binding to EGFR: in silico site-directed mutagenesis and molecular dynamics simulation approaches.
Q61445154An improved yeast surface display platform for the screening of nanobody immune libraries
Q38640630Anatomy and behavioral function of serotonin receptors in Drosophila melanogaster larvae
Q34244067Applications of isothermal titration calorimetry in pure and applied research--survey of the literature from 2010.
Q92370937Architecture of the heteromeric GluA1/2 AMPA receptor in complex with the auxiliary subunit TARP γ8
Q101166607BAcTrace, a tool for retrograde tracing of neuronal circuits in Drosophila
Q96126992Bacteriophage K1F targets Escherichia coli K1 in cerebral endothelial cells and influences the barrier function
Q51646294Bimolecular complementation affinity purification (BiCAP) reveals dimer-specific protein interactions for ERBB2 dimers.
Q37887077Biotechnological applications of recombinant single-domain antibody fragments
Q36952338CAMKII and calcineurin regulate the lifespan of Caenorhabditis elegans through the FOXO transcription factor DAF-16.
Q52597927CD38 produces nicotinic acid adenosine dinucleotide phosphate in the lysosome.
Q88588639CHD3 and CHD4 recruitment and chromatin remodeling activity at DNA breaks is promoted by early poly(ADP-ribose)-dependent chromatin relaxation
Q42148846CRISPR-UnLOCK: Multipurpose Cas9-Based Strategies for Conversion of Yeast Libraries and Strains.
Q35217452Characterization of camel nanobodies specific for superfolder GFP fusion proteins.
Q38774145Computational de novo design of antibodies binding to a peptide with high affinity
Q98728924Cryo-EM analysis of PIP2 regulation in mammalian GIRK channels
Q96135522Cryo-EM structure of the Hedgehog release protein Dispatched
Q52559226Current Conjugation Methods for Immunosensors.
Q50328981Cytosolic interaction of type III human CD38 with CIB1 modulates cellular cyclic ADP-ribose levels
Q57153658DARPins recognizing mTFP1 as novel reagents for and protein manipulations
Q89013052Dimer-specific immunoprecipitation of active caspase-2 identifies TRAF proteins as novel activators
Q49923855Display of Recombinant Proteins on Bacterial Outer Membrane Vesicles Using Protein Ligation.
Q55421652DisruPPI: structure-based computational redesign algorithm for protein binding disruption.
Q89472304Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)
Q91912743Domain swapping of complementarity-determining region in nanobodies produced by Pichia pastoris
Q89546842Dynactin p150 promotes processive motility of DDB complexes by minimizing diffusional behavior of dynein
Q36463359Dynamic relocation of the TORC1-Gtr1/2-Ego1/2/3 complex is regulated by Gtr1 and Gtr2.
Q47727238Engineered Ferritin for Magnetogenetic Manipulation of Proteins and Organelles Inside Living Cells
Q92709664Engineering a Polyspecific Pyrrolysyl-tRNA Synthetase by a High Throughput FACS Screen
Q89971595Engineering a Proximity-Directed O-GlcNAc Transferase for Selective Protein O-GlcNAcylation in Cells
Q64123184Enhanced in vivo-imaging in medaka by optimized anaesthesia, fluorescent protein selection and removal of pigmentation
Q64986573Entropic Control of Receptor Recycling Using Engineered Ligands.
Q50438595Estimation of interaction between oriented immobilized green fluorescent protein and its antibody by high performance affinity chromatography and molecular docking
Q48107843Evaluation of Nanobody Conjugates and Protein Fusions as Bioanalytical Reagents.
Q34441797GFP facilitates native purification of recombinant perlucin derivatives and delays the precipitation of calcium carbonate
Q30418939Identification of cross-linked peptides from complex samples.
Q36959363Immuno-targeting the multifunctional CD38 using nanobody
Q64065348In Vivo Force Application Reveals a Fast Tissue Softening and External Friction Increase during Early Embryogenesis
Q47257394Intein-mediated assembly of tunable scaffoldins for facile synthesis of designer cellulosomes
Q38914281Minimalist Antibodies and Mimetics: An Update and Recent Applications
Q48503113Motor Reattachment Kinetics Play a Dominant Role in Multimotor-Driven Cargo Transport
Q33919839Multicolored nanometre-resolution mapping of single protein-ligand binding complexes using far-field photostable optical nanoscopy (PHOTON).
Q41098832Multifunctional Transmembrane Protein Ligands for Cell-Specific Targeting of Plasma Membrane-Derived Vesicles
Q52684280Nanobody-Displaying Flagellar Nanotubes.
Q96647703Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium
Q49166359Nanobody-triggered lockdown of VSRs reveals ligand reloading in the Golgi.
Q39149504Nanomolar oligomerization and selective co-aggregation of α-synuclein pathogenic mutants revealed by single-molecule fluorescence
Q59136902Nuclear Import of Poly(ADP-Ribose) Polymerase 2 Is Mediated by Importin-α and a Nuclear Localization Sequence Located Between the Predicted SAP Domains
Q88917056Oligonucleotide-mediated tRNA sequestration enables one-pot sense codon reassignment in vitro
Q42676538Patterning protein complexes on DNA nanostructures using a GFP nanobody.
Q56888602Probing Mitotic CENP-E Kinesin with the Tethered Cargo Motion Assay and Laser Tweezers
Q90016362Rapid Delivery of Nanobodies/VHHs into Living Cells via Expressing In Vitro-Transcribed mRNA
Q34157641Rapid mapping of interactions between Human SNX-BAR proteins measured in vitro by AlphaScreen and single-molecule spectroscopy.
Q48042401Receptor-mediated sorting of soluble vacuolar proteins ends at the trans-Golgi network/early endosome
Q101335200Recombinant Antibodies in Basic Neuroscience Research
Q50026240Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents.
Q42062050Resurfaced cell-penetrating nanobodies: A potentially general scaffold for intracellularly targeted protein discovery
Q47635783Rewiring the Budding Yeast Proteome using Synthetic Physical Interactions
Q30664602SNAP23/25 and VAMP2 mediate exocytic event of transferrin receptor-containing recycling vesicles.
Q36945870Scratching the Surface: Resurfacing Proteins to Endow New Properties and Function
Q47277481Sculpting ion channel functional expression with engineered ubiquitin ligases.
Q42138889Self-assembled amyloid-like oligomeric-cohesin Scaffoldin for augmented protein display on the saccharomyces cerevisiae cell surface
Q35590078Single domain antibodies as a powerful tool for high quality surface plasmon resonance studies
Q48663381Single-molecule microscopy of molecules tagged with GFP or RFP derivatives in mammalian cells using nanobody binders.
Q88461941Spatial and temporal organization of cadherin in punctate adherens junctions
Q35693707Specific GFP-binding artificial proteins (αRep): a new tool for in vitro to live cell applications
Q90999189Structural and thermodynamic basis for the recognition of the substrate-binding cleft on hen egg lysozyme by a single-domain antibody
Q89433856Structural basis of VHH-mediated neutralization of the food-borne pathogen Listeria monocytogenes
Q90294042Structural basis of sterol recognition by human hedgehog receptor PTCH1
Q90776062Structural insights into tetraspanin CD9 function
Q90594656Structure and development of single domain antibodies as modules for therapeutics and diagnostics
Q91816763Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification
Q30524349Structure-based reassessment of the caveolin signaling model: do caveolae regulate signaling through caveolin-protein interactions?
Q37367143Subdiffractional tracking of internalized molecules reveals heterogeneous motion states of synaptic vesicles
Q28535595Subunit organisation of in vitro reconstituted HOPS and CORVET multisubunit membrane tethering complexes
Q27936465The Carboxy-Terminal Tails of Septins Cdc11 and Shs1 Recruit Myosin-II Binding Factor Bni5 to the Bud Neck in Saccharomyces cerevisiae.
Q47769245The Display of Single-Domain Antibodies on the Surfaces of Connectosomes Enables Gap Junction-Mediated Drug Delivery to Specific Cell Populations
Q52375209The MYO6 interactome reveals adaptor complexes coordinating early endosome and cytoskeletal dynamics.
Q37285409The Munc18-1 domain 3a hinge-loop controls syntaxin-1A nanodomain assembly and engagement with the SNARE complex during secretory vesicle priming
Q34644530Tunable nano-oleosomes derived from engineered Yarrowia lipolytica.
Q52604635Ultrastructural localisation of protein interactions using conditionally stable nanobodies.
Q90637875Unintended perturbation of protein function using GFP nanobodies in human cells
Q38644638Use of fluorescence-detected sedimentation velocity to study high-affinity protein interactions

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