Crystal structure at 2.4 angstroms resolution of the complex of transducin betagamma and its regulator, phosducin

scientific article

Crystal structure at 2.4 angstroms resolution of the complex of transducin betagamma and its regulator, phosducin is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1016/S0092-8674(00)81376-8
P698PubMed publication ID8898209

P50authorRachelle GaudetQ28914808
P2093author name stringPaul B Sigler
Andrew Bohm
P2860cites workPhosducin-like protein: an ethanol-responsive potential modulator of guanine nucleotide-binding protein functionQ24564134
Improved methods for building protein models in electron density maps and the location of errors in these modelsQ26776980
Phase annealing in SHELX-90: direct methods for larger structuresQ26778409
Crystal structure of thioredoxin from Escherichia coli at 1.68 A resolutionQ27671419
GTPase mechanism of Gproteins from the 1.7-A crystal structure of transducin alpha-GDP-AIF-4Q27730775
Structures of active conformations of Gi alpha 1 and the mechanism of GTP hydrolysisQ27730891
Structural determinants for activation of the alpha-subunit of a heterotrimeric G proteinQ27731423
The 2.2 A crystal structure of transducin-alpha complexed with GTP gamma SQ27731495
The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma 2Q27732195
The 2.0 A crystal structure of a heterotrimeric G proteinQ27732250
Crystal structure of a G-protein beta gamma dimer at 2.1A resolutionQ27732270
Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical featuresQ27860675
The ancient regulatory-protein family of WD-repeat proteinsQ28248516
GAIP and RGS4 are GTPase-activating proteins for the Gi subfamily of G protein alpha subunitsQ28286818
A direct interaction between G-protein beta gamma subunits and the Raf-1 protein kinaseQ28303284
Algorithm for ribbon models of proteinsQ29028460
Culture medium for enterobacteriaQ29616466
Thioredoxin--a fold for all reasonsQ29618984
Rat pineal gland phosducin: cDNA isolation, nucleotide sequence, and chromosomal assignment in the mouseQ33658879
Role of beta gamma subunits of G proteins in targeting the beta-adrenergic receptor kinase to membrane-bound receptorsQ34237289
New roles for G-protein beta gamma-dimers in transmembrane signalling.Q34358634
The N terminus of phosducin is involved in binding of beta gamma subunits of G proteinQ34566626
Repetitive segmental structure of the transducin beta subunit: homology with the CDC4 gene and identification of related mRNAsQ35597697
Phosducin is a ubiquitous G-protein regulator.Q37328331
Inhibition of G-protein betagamma-subunit functions by phosducin-like protein.Q37710136
Regulation of phosducin phosphorylation in retinal rods by Ca2+/calmodulin-dependent adenylyl cyclaseQ37717974
Analysis of phosducin as a candidate gene for retinopathiesQ38360930
PH domains: diverse sequences with a common fold recruit signaling molecules to the cell surfaceQ40996433
Interactions of phosducin with defined G protein beta gamma-subunitsQ42064300
Phosducin and PP33 are in vivo targets of PKA and type 1 or 2A phosphatases, regulators of cell elongation in teleost rod inner-outer segmentsQ46052627
The photoreceptor-specific 33 kDa phosphoprotein of mammalian retina: generation of monospecific antibodies and localization by immunocytochemistryQ46488034
Regulation of the kinetics of phosducin phosphorylation in retinal rodsQ46536084
Retinal accumulation of the phosducin/T beta gamma and transducin complexes in developing normal mice and in mice and dogs with inherited retinal degenerationQ46731817
Protein hydration observed by X-ray diffraction. Solvation properties of penicillopepsin and neuraminidase crystal structures.Q47650854
Phosducin is a protein kinase A-regulated G-protein regulatorQ48456911
Cyclic GMP and photoreceptor functionQ55042450
Improved Fourier coefficients for maps using phases from partial structures with errorsQ56877556
MEKA/phosducin attenuates hydrophobicity of transducin beta gamma subunits without binding to farnesyl moietyQ71209923
Aluminum fluoride activation of bovine transducin induces two distinct conformational changes in the alpha subunitQ71640778
Purification of T beta gamma subunit of transducinQ72738760
P433issue3
P407language of work or nameEnglishQ1860
P921main subjectcrystal structureQ895901
P304page(s)577-88
577-588
P577publication date1996-11-01
P1433published inCellQ655814
P1476titleCrystal Structure at 2.4 Å Resolution of the Complex of Transducin βγ and Its Regulator, Phosducin
Crystal structure at 2.4 angstroms resolution of the complex of transducin betagamma and its regulator, phosducin
P478volume87

Reverse relations

cites work (P2860)
Q42097716A computational model predicts that Gβγ acts at a cleft between channel subunits to activate GIRK1 channels.
Q34412814A conserved protein interaction interface on the type 5 G protein beta subunit controls proteolytic stability and activity of R7 family regulator of G protein signaling proteins
Q34181731A genome-wide linkage and association scan reveals novel loci for hypertension and blood pressure traits.
Q36095137A groovy new structure
Q27618511A molecular mechanism for the phosphorylation-dependent regulation of heterotrimeric G proteins by phosducin
Q42627313A small region in phosducin inhibits G-protein betagamma-subunit function.
Q41587222Activation and inactivation steps in the visual transduction pathway
Q90253107Archaeal Unfoldase Counteracts Protein Misfolding Retinopathy in Mice
Q58699689Biophysical and structural characterization of the thermostable WD40 domain of a prokaryotic protein, Thermomonospora curvata PkwA
Q33729021CK2 phosphorylation of human centrins 1 and 2 regulates their binding to the DNA repair protein XPC, the centrosomal protein Sfi1 and the phototransduction protein transducin β
Q77325929Calcium regulation of phototransduction in vertebrate rod outer segments
Q60924700Characterization of a protozoan Phosducin-like protein-3 (PhLP-3) reveals conserved redox activity
Q32035033Characterization of canine photoreceptor phosducin cDNA and identification of a sequence variant in dogs with photoreceptor dysplasia
Q22009101Cloning and characterization of the rat and human phosducin-like protein genes: structure, expression and chromosomal localization
Q64074411Comparative Genomics of HRh1 and sp. SCN-R1, Halophilic Chemolithoautotrophic Sulfur-Oxidizing Capable of Using Thiocyanate as Energy Source
Q44930204Compartment-specific phosphorylation of phosducin in rods underlies adaptation to various levels of illumination
Q24329157Core histones and HIRIP3, a novel histone-binding protein, directly interact with WD repeat protein HIRA
Q36026254Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination
Q27639147Crystal structure of the C-terminal WD40 repeat domain of the human Groucho/TLE1 transcriptional corepressor
Q27621706Crystal structure of the human U4/U6 small nuclear ribonucleoprotein particle-specific SnuCyp-20, a nuclear cyclophilin
Q27649555Crystal structure of the multifunctional Gβ5–RGS9 complex
Q37258536Differential inhibitor of Gbetagamma signaling to AKT and ERK derived from phosducin-like protein: effect on sphingosine 1-phosphate-induced endothelial cell migration and in vitro angiogenesis
Q30434268Differential sensitivity of phosphatidylinositol 3-kinase p110gamma to isoforms of G protein betagamma dimers
Q34454850Disruption of the Chaperonin Containing TCP-1 Function Affects Protein Networks Essential for Rod Outer Segment Morphogenesis and Survival
Q36325880Distinct roles for two Galpha-Gbeta interfaces in cell polarity control by a yeast heterotrimeric G protein
Q42523857Distinct sites on G protein beta gamma subunits regulate different effector functions
Q44132286Distribution of rod- and cone-specific phosducins in retinas of non-mammalian vertebrates
Q42732257Diversity of functions of proteins with internal symmetry in spatial arrangement of secondary structural elements
Q48488375Dominant negative effects of a Gbeta mutant on G-protein coupled inward rectifier K+ channel.
Q36737780Dopamine modulates diurnal and circadian rhythms of protein phosphorylation in photoreceptor cells of mouse retina.
Q35669441Drosophila NURF-55, a WD repeat protein involved in histone metabolism
Q45864104Effects of two Gbetagamma-binding proteins--N-terminally truncated phosducin and beta-adrenergic receptor kinase C terminus (betaARKct)--in heart failure
Q43115191Electrostatic and lipid anchor contributions to the interaction of transducin with membranes: mechanistic implications for activation and translocation.
Q37526805Embryonic markers of cone differentiation
Q27667530Escherichia coli Thioredoxin-like Protein YbbN Contains an Atypical Tetratricopeptide Repeat Motif and Is a Negative Regulator of GroEL
Q37233080Evasion of ribonuclease inhibitor as a determinant of ribonuclease cytotoxicity
Q24545591Evidence that a protein-protein interaction 'hot spot' on heterotrimeric G protein betagamma subunits is used for recognition of a subclass of effectors
Q36666920Exome sequencing identifies GNB4 mutations as a cause of dominant intermediate Charcot-Marie-Tooth disease.
Q43842112Exploring protein-protein interactions by peptide docking protocols
Q49448661Farnesylation of the Transducin G Protein Gamma Subunit Is a Prerequisite for Its Ciliary Targeting in Rod Photoreceptors
Q34656739Function of phosducin-like proteins in G protein signaling and chaperone-assisted protein folding
Q27932729Functional analysis of Plp1 and Plp2, two homologues of phosducin in yeast
Q24681339Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeletal protein function and cell cycle progression
Q74008633Functional roles of the two domains of phosducin and phosducin-like protein
Q40436463G protein beta2 subunit-derived peptides for inhibition and induction of G protein pathways. Examination of voltage-gated Ca2+ and G protein inwardly rectifying K+ channels
Q46391444G protein betagamma directly regulates SNARE protein fusion machinery for secretory granule exocytosis.
Q37184794G protein betagamma subunits as targets for small molecule therapeutic development
Q34639816G protein modulation of CaV2 voltage-gated calcium channels
Q37167274G protein βγ subunits: central mediators of G protein-coupled receptor signaling
Q36914223G protein-coupled receptor-promoted trafficking of Gbeta1gamma2 leads to AKT activation at endosomes via a mechanism mediated by Gbeta1gamma2-Rab11a interaction
Q34400860Gating of a G protein-sensitive mammalian Kir3.1 prokaryotic Kir channel chimera in planar lipid bilayers
Q42512845Gbeta residues that do not interact with Galpha underlie agonist-independent activity of K+ channels
Q40331264Gbetagamma inhibits Galpha GTPase-activating proteins by inhibition of Galpha-GTP binding during stimulation by receptor
Q52995859Gbetagamma stimulates phosphoinositide 3-kinase-gamma by direct interaction with two domains of the catalytic p110 subunit.
Q50099690Genome-Wide Identification and Characterization of WD40 Protein Genes in the Silkworm, Bombyx mori
Q37519403Genome-wide Analysis of WD40 Protein Family in Human.
Q74164937How Gsalpha activates adenylyl cyclase
Q34481540Hydrogen-bonding dynamics between adjacent blades in G-protein beta-subunit regulates GIRK channel activation
Q24296686Identification of PDCL3 as a novel chaperone protein involved in the generation of functional VEGF receptor 2
Q47970470Identification of a potassium channel site that interacts with G protein betagamma subunits to mediate agonist-induced signaling
Q42060840Identification of a structural element in phospholipase C beta2 that interacts with G protein betagamma subunits
Q28354268Identification of a structural motif that confers specific interaction with the WD40 repeat domain of Arabidopsis COP1.
Q37155365Identification of bdm-1, a gene involved in G protein beta-subunit function and alpha-subunit accumulation
Q28210562Identification of critical residues controlling G protein-gated inwardly rectifying K(+) channel activity through interactions with the beta gamma subunits of G proteins
Q27938799Identification of residues in the WD-40 repeat motif of the F-box protein Met30p required for interaction with its substrate Met4p
Q38296285Identification of rod- and cone-specific phosducins in teleost retinas.
Q22010983Identification, characterization and crystal structure analysis of the human spliceosomal U5 snRNP-specific 15 kD protein
Q34391209Identifying the hotspots on the top faces of WD40-repeat proteins from their primary sequences by β-bulges and DHSW tetrads
Q44628546Interaction of G Protein β Subunit with Inward Rectifier K+Channel Kir3
Q46176657Interaction of phosducin-like protein with G protein betagamma subunits
Q36987728Intrinsic Pleckstrin Homology (PH) Domain Motion in Phospholipase C-β Exposes a Gβγ Protein Binding Site
Q24311782Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit
Q27641311Keeping G proteins at bay: a complex between G protein-coupled receptor kinase 2 and Gbetagamma
Q34772096Light-dependent compartmentalization of transducin in rod photoreceptors.
Q28139341Lissencephaly associated mutations suggest a requirement for the PAFAH1B heterotrimeric complex in brain development
Q27936013Localization and signaling of G(beta) subunit Ste4p are controlled by a-factor receptor and the a-specific protein Asg7p
Q52569702MAU-8 is a Phosducin-like Protein required for G protein signaling in C. elegans.
Q41881490Mapping of a yeast G protein betagamma signaling interaction.
Q27919700Mechanism of assembly of G protein betagamma subunits by protein kinase CK2-phosphorylated phosducin-like protein and the cytosolic chaperonin complex
Q38176851Mechanisms for regulating deubiquitinating enzymes
Q35995003Mechanistic pathways and biological roles for receptor-independent activators of G-protein signaling.
Q24684714Model of the brain tumor-Pumilio translation repressor complex
Q28186591Modulation of the G protein regulator phosducin by Ca2+/calmodulin-dependent protein kinase II phosphorylation and 14-3-3 protein binding
Q28141071Molecular cloning and characterization of the human phosducin-like protein (hPhLP) promoter
Q35998387Molecular determinants of inactivation and G protein modulation in the intracellular loop connecting domains I and II of the calcium channel alpha1A subunit
Q22011044Mutations in host cell factor 1 separate its role in cell proliferation from recruitment of VP16 and LZIP
Q61444192Mutual action by Gγ and Gβ for optimal activation of GIRK channels in a channel subunit-specific manner
Q33640120NMR analysis of G-protein betagamma subunit complexes reveals a dynamic G(alpha)-Gbetagamma subunit interface and multiple protein recognition modes
Q24802819No statistical support for correlation between the positions of protein interaction sites and alternatively spliced regions
Q28590012NudE-L, a novel Lis1-interacting protein, belongs to a family of vertebrate coiled-coil proteins
Q35587760Organization and functions of interacting domains for signaling by protein-protein interactions
Q55395928PPI network analyses of human WD40 protein family systematically reveal their tendency to assemble complexes and facilitate the complex predictions.
Q27934103PhLP3 modulates CCT-mediated actin and tubulin folding via ternary complexes with substrates
Q43842064Phosducin down-regulation of G-protein coupling: reconstitution of phosducin and transducin of cGMP cascade in bovine rod photoreceptor cells
Q55477964Phosducin expression in NG 108-15 hybrid cells enhances prostaglandin E1 stimulated adenylate cyclase activity.
Q44770010Phosducin facilitates light-driven transducin translocation in rod photoreceptors. Evidence from the phosducin knockout mouse
Q27765335Phosducin induces a structural change in transducin beta gamma
Q37452264Phosducin influences sympathetic activity and prevents stress-induced hypertension in humans and mice
Q36296057Phosducin regulates the expression of transducin betagamma subunits in rod photoreceptors and does not contribute to phototransduction adaptation
Q24529136Phosducin-like protein acts as a molecular chaperone for G protein betagamma dimer assembly
Q28237913Phosducin-like protein regulates G-protein betagamma folding by interaction with tailless complex polypeptide-1alpha: dephosphorylation or splicing of PhLP turns the switch toward regulation of Gbetagamma folding
Q39927867Phosducin-like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins.
Q36056151Phosphorylation of phosducin accelerates rod recovery from transducin translocation
Q28140208Phosphorylation of phosducin and phosducin-like protein by G protein-coupled receptor kinase 2
Q30472460Phosphorylation of the G protein gamma12 subunit regulates effector specificity
Q35296090Phototransduction: crystal clear
Q39958681Prediction of protein-protein interfaces on G-protein beta subunits reveals a novel phospholipase C beta2 binding domain
Q43634320Protein disulfide oxidoreductase from Pyrococcus furiosus: structural properties
Q37138123Protein sorting, targeting and trafficking in photoreceptor cells
Q34081480Protein-protein interactions in receptor activation and intracellular signalling
Q53582665RACK1 (receptor for activated C-kinase 1) interactions with spectrin repeat elements.
Q42040223RACK1 binds to a signal transfer region of G betagamma and inhibits phospholipase C beta2 activation
Q30407898RACK1, A multifaceted scaffolding protein: Structure and function
Q24315691RACK1, a receptor for activated C kinase and a homolog of the beta subunit of G proteins, inhibits activity of src tyrosine kinases and growth of NIH 3T3 cells
Q47735328Receptor- and nucleotide exchange-independent mechanisms for promoting G protein subunit dissociation.
Q36565441Regulation of Ca(V)2 calcium channels by G protein coupled receptors.
Q44055094Regulation of angiotensin II-induced G protein signaling by phosducin-like protein
Q47821875Regulation of exocytosis in chromaffin cells by phosducin-like protein, a protein interacting with G protein betagamma subunits
Q28216669Regulation of phosducin-like protein by casein kinase 2 and N-terminal splicing
Q28211377Regulator of G-protein signaling 3 (RGS3) inhibits Gbeta1gamma 2-induced inositol phosphate production, mitogen-activated protein kinase activation, and Akt activation
Q34198530Regulators of G-protein signaling and their Gα substrates: promises and challenges in their use as drug discovery targets
Q24534363Regulatory interaction of phosducin-like protein with the cytosolic chaperonin complex
Q36573183Residues in the WD repeats of Tup1 required for interaction with alpha2.
Q24635169Rethinking the role of phosducin: light-regulated binding of phosducin to 14-3-3 in rod inner segments
Q30305912Role of C-terminal domains of the G protein beta subunit in the activation of effectors.
Q24317450Role of molecular chaperones in G protein beta5/regulator of G protein signaling dimer assembly and G protein betagamma dimer specificity
Q34437374Role of subunit diversity in signaling by heterotrimeric G proteins
Q43933148Role of the G protein gamma subunit in beta gamma complex modulation of phospholipase Cbeta function.
Q28292447SUMO-1 controls the protein stability and the biological function of phosducin
Q32004554Selection of gbeta subunits with point mutations that fail to activate specific signaling pathways in vivo: dissecting cellular responses mediated by a heterotrimeric G protein in Dictyostelium discoideum
Q52171360Short communication: the N-terminal fragment of Arabidopsis photomorphogenic repressor COP1 maintains partial function and acts in a concentration-dependent manner.
Q45107797Site-specific phosphorylation of phosducin in intact retina. Dynamics of phosphorylation and effects on G protein beta gamma dimer binding
Q34472422Sites for Galpha binding on the G protein beta subunit overlap with sites for regulation of phospholipase Cbeta and adenylyl cyclase
Q34475423Sites important for PLCbeta2 activation by the G protein betagamma subunit map to the sides of the beta propeller structure
Q37150989Splice isoforms of phosducin-like protein control the expression of heterotrimeric G proteins
Q44370228Stimulation of cellular signaling and G protein subunit dissociation by G protein betagamma subunit-binding peptides.
Q51062260Structural Basis for the 14-3-3 Protein-Dependent Inhibition of Phosducin Function.
Q40942320Structural Characterization of Phosducin and Its Complex with the 14-3-3 Protein.
Q41572794Structural aspects of heterotrimeric G-protein signaling
Q24314963Structural basis for the specific recognition of methylated histone H3 lysine 4 by the WD-40 protein WDR5
Q77350803Structural basis of activity and subunit recognition in G protein heterotrimers
Q27640256Structural basis of inward rectification: cytoplasmic pore of the G protein-gated inward rectifier GIRK1 at 1.8 A resolution
Q47614473Structural characterisation and functional significance of transient protein-protein interactions
Q33661228Structural features of heterotrimeric G-protein-coupled receptors and their modulatory proteins
Q36379125Structural modulation of phosducin by phosphorylation and 14-3-3 protein binding
Q27638103Structural views of the ligand-binding cores of a metabotropic glutamate receptor complexed with an antagonist and both glutamate and Gd3+.
Q27667443Structure and function of WD40 domain proteins
Q34298694Structure of Ca2+ release channel at 14 A resolution
Q24306203Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity of the SCF(beta-TrCP1) ubiquitin ligase
Q33869703Structure of an apoptosome-procaspase-9 CARD complex.
Q91330297Structure of an endosomal signaling GPCR-G protein-β-arrestin megacomplex
Q28206676Structure of rhodopsin and the superfamily of seven-helical receptors: the same and not the same
Q24678778Structure of the C-terminal domain of Tup1, a corepressor of transcription in yeast
Q37713810Structure of the complex between the cytosolic chaperonin CCT and phosducin-like protein.
Q41791755Structure of the parathyroid hormone receptor C terminus bound to the G-protein dimer Gbeta1gamma2.
Q27653651Structure of the thioredoxin-fold domain of human phosducin-like protein 2
Q35134639Structures of the Gβ-CCT and PhLP1-Gβ-CCT complexes reveal a mechanism for G-protein β-subunit folding and Gβγ dimer assembly
Q34603662Substitutions in the pheromone-responsive Gbeta protein of Saccharomyces cerevisiae confer a defect in recovery from pheromone treatment
Q93064600TXNDC9 regulates oxidative stress-induced androgen receptor signaling to promote prostate cancer progression
Q37831634Taking the heart failure battle inside the cell: small molecule targeting of Gβγ subunits
Q91827049Targeting G protein-coupled receptor signalling by blocking G proteins
Q37124472The BARD1 C-terminal domain structure and interactions with polyadenylation factor CstF-50.
Q91809881The Emerging Role of Gβ Subunits in Human Genetic Diseases
Q28143827The WD repeat: a common architecture for diverse functions
Q30480605The WD-repeats of Net2p interact with Dnm1p and Fis1p to regulate division of mitochondria
Q33370697The information highways of a biotechnological workhorse--signal transduction in Hypocrea jecorina
Q41906186The phosducin-like protein PhLP1 is essential for G{beta}{gamma} dimer formation in Dictyostelium discoideum
Q37808650The physiological roles of phosducin: from retinal function to stress-dependent hypertension
Q33543247The protein disulphide-isomerase family: unravelling a string of folds
Q34638080The role of G proteins in assembly and function of Kir3 inwardly rectifying potassium channels
Q43738133The role of electrostatic interactions in the regulation of the membrane association of G protein beta gamma heterodimers
Q55035703Three-dimensional structure of the apoptosome: implications for assembly, procaspase-9 binding, and activation.
Q48450479Translocation of phosducin in living neuroblastoma x glioma hybrid cells (NG 108-15) monitored by red-shifted green fluorescent protein
Q30420687Transport and localization of signaling proteins in ciliated cells
Q28221260Ubiquitylation of the transducin betagamma subunit complex. Regulation by phosducin
Q24635233Understanding molecular recognition by G protein βγ subunits on the path to pharmacological targeting
Q31541796Vertebrate photoreceptors
Q35253276WDSPdb: a database for WD40-repeat proteins
Q24534990Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes
Q28512956sel-10, a negative regulator of lin-12 activity in Caenorhabditis elegans, encodes a member of the CDC4 family of proteins