Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus

scientific article (publication date: November 1999)

Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus is …
instance of (P31):
scholarly articleQ13442814

External links are
P356DOI10.1093/OXFORDJOURNALS.JBCHEM.A022539
P8608Fatcat IDrelease_mfckosv2qngg7fcryrsmzu4q4y
P3181OpenCitations bibliographic resource ID1859454
P698PubMed publication ID10544290
P5875ResearchGate publication ID12757088

P2093author name stringT Yano
S Kuramitsu
H Kagamiyama
Y Koyama
J Hoseki
P433issue5
P407language of work or nameEnglishQ1860
P921main subjectThermus thermophilusQ139470
directed evolutionQ440681
P304page(s)951-6
P577publication date1999-11-01
P1433published inJournal of BiochemistryQ6294839
P1476titleDirected evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus
P478volume126

Reverse relations

cites work (P2860)
Q42406914A Highly Thermostable Kanamycin Resistance Marker Expands the Tool Kit for Genetic Manipulation of Caldicellulosiruptor bescii
Q43031094A Key Enzyme of the NAD+ Salvage Pathway in Thermus thermophilus: Characterization of Nicotinamidase and the Impact of Its Gene Deletion at High Temperatures
Q90139121A Modular Vector Toolkit with a Tailored Set of Thermosensors To Regulate Gene Expression in Thermus thermophilus
Q36871387A markerless gene deletion and integration system for Thermoanaerobacter ethanolicus
Q38328624A non-cold-inducible cold shock protein homolog mainly contributes to translational control under optimal growth conditions.
Q34225001A novel enzymatic system against oxidative stress in the thermophilic hydrogen-oxidizing bacterium Hydrogenobacter thermophilus
Q42423547A novel single-stranded DNA-specific 3'-5' exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways
Q43025704A reporter gene system for the precise measurement of promoter activity in Thermus thermophilus HB27.
Q34060931A selection that reports on protein-protein interactions within a thermophilic bacterium
Q41903850An Extreme Thermophile,Thermus thermophilus, Is a Polyploid Bacterium
Q38290938An O6-methylguanine-DNA methyltransferase-like protein from Thermus thermophilus interacts with a nucleotide excision repair protein
Q41786210Application of pheB as a reporter gene for Geobacillus spp., enabling qualitative colony screening and quantitative analysis of promoter strength
Q35161643Assessing directed evolution methods for the generation of biosynthetic enzymes with potential in drug biosynthesis.
Q30327881Automated search of natively folded protein fragments for high-throughput structure determination in structural genomics.
Q37767245Biotechnological approaches to develop bacterial chitinases as a bioshield against fungal diseases of plants.
Q46726051Bound nucleotide controls the endonuclease activity of mismatch repair enzyme MutL.
Q48177430Cre/lox-based multiple markerless gene disruption in the genome of the extreme thermophile Thermus thermophilus.
Q27652436Crystal Structure of MutS2 Endonuclease Domain and the Mechanism of Homologous Recombination Suppression
Q38680095Curing the Megaplasmid pTT27 from Thermus thermophilus HB27 and Maintaining Exogenous Plasmids in the Plasmid-Free Strain
Q43029466Determining the roles of the three alcohol dehydrogenases (AdhA, AdhB and AdhE) in Thermoanaerobacter ethanolicus during ethanol formation.
Q30649929Directed evolution of ampicillin-resistant activity from a functionally unrelated DNA fragment: A laboratory model of molecular evolution
Q51636985Directed evolution of bacteriorhodopsin for device applications.
Q37654906Directed evolution: selection of the host organism
Q34029169Directed evolution: the 'rational' basis for 'irrational' design.
Q34596533Discovery of superior enzymes by directed molecular evolution
Q43029438Disruption of Thermus thermophilus genes by homologous recombination using a thermostable kanamycin-resistant marker
Q58611530Ecological selection for small microbial genomes along a temperate-to-thermal soil gradient
Q34365202Engineering proteins for thermostability: the use of sequence alignments versus rational design and directed evolution
Q35107273Engineering the genome of Thermus thermophilus using a counterselectable marker
Q54417060Enzymatic analysis of a thermostabilized mutant of an Escherichia coli hygromycin B phosphotransferase.
Q33927034Evolutionary optimisation of enzymes
Q39502377Experimental evolution of enzyme temperature activity profile: selection in vivo and characterization of low-temperature-adapted mutants of Pyrococcus furiosus ornithine carbamoyltransferase
Q26776556Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals
Q43027537Folate-/FAD-dependent tRNA methyltransferase from Thermus thermophilus regulates other modifications in tRNA at low temperatures.
Q54202780Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae.
Q43015618Heterologous gene expression in Thermus thermophilus: beta-galactosidase, dibenzothiophene monooxygenase, PNB carboxy esterase, 2-aminobiphenyl-2,3-diol dioxygenase, and chloramphenicol acetyl transferase
Q28829876Identification of Preferred DNA-Binding Sites for the Thermus thermophilus Transcriptional Regulator SbtR by the Combinatorial Approach REPSA
Q47849527Identification of a rhodanese-like protein involved in thiouridine biosynthesis in Thermus thermophilus tRNA.
Q43019825Identification of two tRNA thiolation genes required for cell growth at extremely high temperatures
Q34427599Improving the quality of industrially important enzymes by directed evolution
Q27674862In Vivo, in Vitro, and X-ray Crystallographic Analyses Suggest the Involvement of an Uncharacterized Triose-phosphate Isomerase (TIM) Barrel Protein in Protection against Oxidative Stress
Q43024969In vitro dihydrouridine formation by tRNA dihydrouridine synthase from Thermus thermophilus, an extreme-thermophilic eubacterium.
Q33892504Inactivation of the DNA repair genes mutS, mutL or the anti-recombination gene mutS2 leads to activation of vitamin B1 biosynthesis genes
Q44992380Inhibition of hepatitis B virus DNA replication by a thermostable interferon-γ variant
Q30731663Investigating and Engineering Enzymes by Genetic Selection
Q42039091Involvement of CarA/LitR and CRP/FNR family transcriptional regulators in light-induced carotenoid production in Thermus thermophilus
Q56898721Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability
Q45308849Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus
Q43030798Long and branched polyamines are required for maintenance of the ribosome, tRNAHis and tRNATyr in Thermus thermophilus cells at high temperatures.
Q47176216Low molecular weight thiols and thioredoxins are important players in Hg(II) resistance in Thermus thermophilus HB27.
Q38387024Markerless Gene Deletion with Cytosine Deaminase in Thermus thermophilus Strain HB27.
Q27662428Multi-site-specific 16S rRNA methyltransferase RsmF from Thermus thermophilus
Q33633751N7-Methylguanine at position 46 (m7G46) in tRNA from Thermus thermophilus is required for cell viability at high temperatures through a tRNA modification network
Q37610278Novel thermostable antibiotic resistance enzymes from the Atlantis II Deep Red Sea brine pool.
Q39488941Overexpression, purification and characterization of RecJ protein from Thermus thermophilus HB8 and its core domain
Q52759799Phosphoserine Phosphatase Is Required for Serine and One-Carbon Unit Synthesis in Hydrogenobacter thermophilus.
Q36006888Posttranslational modification of cellular proteins by a ubiquitin-like protein in bacteria.
Q42056393Pseudouridine at position 55 in tRNA controls the contents of other modified nucleotides for low-temperature adaptation in the extreme-thermophilic eubacterium Thermus thermophilus
Q64114364Regulatory Factors for tRNA Modifications in Extreme- Thermophilic Bacterium
Q57753583Resistance to UV Irradiation Caused by Inactivation of and Genes in Thermus thermophilus
Q42133451Ribosome engineering to promote new crystal forms
Q43024706Roles of Mn-catalase and a possible heme peroxidase homologue in protection from oxidative stress in Thermus thermophilus.
Q35126725Substrate tRNA recognition mechanism of eubacterial tRNA (m1A58) methyltransferase (TrmI).
Q38318304Temperature-dependent biosynthesis of 2-thioribothymidine of Thermus thermophilus tRNA.
Q34306230The Nudix hydrolase Ndx1 from Thermus thermophilus HB8 is a diadenosine hexaphosphate hydrolase with a novel activity
Q35177940The role of ribonucleases in regulating global mRNA levels in the model organism Thermus thermophilus HB8.
Q43034025The third plasmid pVV8 from Thermus thermophilus HB8: isolation, characterization, and sequence determination
Q41762507Thermoadaptation-directed enzyme evolution in an error-prone thermophile derived from Geobacillus kaustophilus HTA426.
Q30374445Thermus thermophilus as biological model.
Q28485549Thermus thermophilus glycoside hydrolase family 57 branching enzyme: crystal structure, mechanism of action, and products formed
Q57091367Uncoupling Fermentative Synthesis of Molecular Hydrogen from Biomass Formation in Thermotoga maritima
Q30984249Understanding thermostability in cytochrome P450 by combinatorial mutagenesis
Q43018050Unique substrate specificity of purine nucleoside phosphorylases from Thermus thermophilus

Search more.