Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies

scientific article published on January 1999

Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies is …
instance of (P31):
scholarly articleQ13442814

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P819ADS bibcode1999PNAS...96..388X
P356DOI10.1073/PNAS.96.2.388
P932PMC publication ID15146
P698PubMed publication ID9892643
P5875ResearchGate publication ID13395761

P50authorDavid CowburnQ41044138
P2093author name stringT W Muir
R Xu
B Ayers
P2860cites workA limited set of SH2 domains binds BCR through a high-affinity phosphotyrosine-independent interactionQ24600945
Mutagenic analysis of the roles of SH2 and SH3 domains in regulation of the Abl tyrosine kinaseQ24612979
Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solutionQ24649924
Expressed protein ligation: a general method for protein engineeringQ24657669
The solution structure of Abl SH3, and its relationship to SH2 in the SH(32) constructQ27732549
Intramolecular interactions of the regulatory domains of the Bcr-Abl kinase reveal a novel control mechanismQ27733413
Determination of macromolecular structures from anomalous diffraction of synchrotron radiationQ27860667
Direct measurement of distances and angles in biomolecules by NMR in a dilute liquid crystalline mediumQ28253146
Synthesis of proteins by native chemical ligationQ29616750
Discovering high-affinity ligands for proteins: SAR by NMRQ29617569
Cytoplasmic signalling domains: the next generationQ30176461
Modular peptide recognition domains in eukaryotic signalingQ30176687
Enhanced affinities and specificities of consolidated ligands for the Src homology (SH) 3 and SH2 domains of Abelson protein-tyrosine kinase.Q30192950
Protein splicing in trans by purified N- and C-terminal fragments of the Mycobacterium tuberculosis RecA inteinQ36004286
Total chemical synthesis of enzymatically active human type II secretory phospholipase A2.Q36296181
Protein folding: local structures, domains, subunits, and assembliesQ37679302
The viral and cellular forms of the Abelson (abl) oncogeneQ39320781
Expressed protein ligation, a novel method for studying protein-protein interactions in transcriptionQ40854749
Rapid in situ neutralization protocols for Boc and Fmoc solid-phase chemistriesQ41629422
Protein synthesis by chemical ligation of unprotected peptides in aqueous solutionQ41629514
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Semisynthesis of cytotoxic proteins using a modified protein splicing elementQ42846179
Probing intermolecular main chain hydrogen bonding in serine proteinase-protein inhibitor complexes: chemical synthesis of backbone-engineered turkey ovomucoid third domainQ46353663
New techniques in structural NMR--anisotropic interactionsQ47841921
NMR of modular proteinsQ47841993
The second decade--into the third milleniumQ47842008
Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing elementQ56267595
Three-dimensional solution structure of the src homology 2 domain of c-ablQ57800449
The leucine zipper domain controls the orientation of AP-1 in the NFAT.AP-1.DNA complexQ67426632
In vivo diagnostic procedures: skin testing, nasal provocation, and bronchial provocationQ73661377
Molecular dissection of the Mycobacterium tuberculosis RecA intein: design of a minimal intein and of a trans-splicing system involving two intein fragmentsQ74342736
Chemical ligation of unprotected peptides directly from a solid supportQ74481443
P433issue2
P407language of work or nameEnglishQ1860
P921main subjectisotopic labelingQ370228
protein foldingQ847556
P304page(s)388-393
P577publication date1999-01-01
P1433published inProceedings of the National Academy of Sciences of the United States of AmericaQ1146531
P1476titleChemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies
P478volume96