SecYEG assembles into a tetramer to form the active protein translocation channel

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SecYEG assembles into a tetramer to form the active protein translocation channel is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1093/EMBOJ/19.5.852
P932PMC publication ID305625
P698PubMed publication ID10698927
P5875ResearchGate publication ID24677868

P2093author name stringA J Driessen
A Engel
E H Manting
H Remigy
C van Der Does
P2860cites workEvolutionary conservation of components of the protein translocation complexQ24323353
SecD and SecF are required for the proton electrochemical gradient stimulation of preprotein translocationQ24596484
Mass Measurement in the Scanning Transmission Electron Microscope: A Powerful Tool for Studying Membrane ProteinsQ32061674
The Sec systemQ33538582
Purified secB protein of Escherichia coli retards folding and promotes membrane translocation of the maltose-binding protein in vitroQ33678296
Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzymeQ33886664
Sec-dependent membrane protein biogenesis: SecYEG, preprotein hydrophobicity and translocation kinetics control the stop-transfer functionQ33888178
Functional identification of the product of the Bacillus subtilis yvaL gene as a SecG homologue.Q33991471
BiP Maintains the Permeability Barrier of the ER Membrane by Sealing the Lumenal End of the Translocon Pore before and Early in TranslocationQ74405725
The bacterial SecY/E translocation complex forms channel-like structures similar to those of the eukaryotic Sec61p complexQ77901565
A novel membrane protein involved in protein translocation across the cytoplasmic membrane of Escherichia coli.Q34058655
A cytoplasmic domain is important for the formation of a SecY-SecE translocator complexQ35241622
Subunit dynamics in Escherichia coli preprotein translocaseQ35271076
Genetic and molecular characterization of the Escherichia coli secD operon and its productsQ36105112
Both an N-terminal 65-kDa domain and a C-terminal 30-kDa domain of SecA cycle into the membrane at SecYEG during translocationQ36151146
Electrochemical potential releases a membrane-bound secretion intermediate of maltose-binding protein in Escherichia coliQ36253218
Classification of images of biomolecular assemblies: a study of ribosomes and ribosomal subunits of Escherichia coliQ36427603
SecYEG and SecA Are the Stoichiometric Components of Preprotein TranslocaseQ38292412
SecD is involved in the release of translocated secretory proteins from the cytoplasmic membrane of Escherichia coliQ38322783
The catalytic cycle of the Escherichia coli SecA ATPase comprises two distinct preprotein translocation eventsQ38339927
Preprotein translocation by a hybrid translocase composed of Escherichia coli and Bacillus subtilis subunits.Q39497981
In vivo cross-linking of the SecA and SecY subunits of the Escherichia coli preprotein translocaseQ39847154
SecF stabilizes SecD and SecY, components of the protein translocation machinery of the Escherichia coli cytoplasmic membraneQ39932560
The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE.Q40805905
The SecA and SecY subunits of translocase are the nearest neighbors of a translocating preprotein, shielding it from phospholipidsQ40871781
Precursor protein translocation by the Escherichia coli translocase is directed by the protonmotive forceQ41518075
Alignment of Conduits for the Nascent Polypeptide Chain in the Ribosome-Sec61 ComplexQ41633493
Bacterial preprotein translocase: mechanism and conformational dynamics of a processive enzyme.Q41715787
The protease-protected 30 kDa domain of SecA is largely inaccessible to the membrane lipid phaseQ41944218
Membrane deinsertion of SecA underlying proton motive force-dependent stimulation of protein translocationQ42213460
Purified Escherichia coli preprotein translocase catalyzes multiple cycles of precursor protein translocationQ43564821
Translocation of ProOmpA possessing an intramolecular disulfide bridge into membrane vesicles of Escherichia coli. Effect of membrane energizationQ43907653
ProOmpA is stabilized for membrane translocation by either purified E. coli trigger factor or canine signal recognition particleQ44556405
SecA protein needs both acidic phospholipids and SecY/E protein for functional high-affinity binding to the Escherichia coli plasma membraneQ44608604
The ATPase activity of SecA is regulated by acidic phospholipids, SecY, and the leader and mature domains of precursor proteinsQ44686930
The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation.Q46008320
The binding cascade of SecB to SecA to SecY/E mediates preprotein targeting to the E. coli plasma membraneQ46059428
ΔμH+ and ATP function at different steps of the catalytic cycle of preprotein translocaseQ46481993
SecA protein is required for secretory protein translocation into E. coli membrane vesiclesQ52455868
SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertionQ52513940
Topology of the Integral Membrane Form of Escherichia coli SecA Protein Reveals Multiple Periplasmically Exposed Regions and Modulation by ATP BindingQ54559159
Inhibition of preprotein translocation and reversion of the membrane inserted state of SecA by a carboxyl terminus binding mAb.Q54561776
Identification of a region of interaction between Escherichia coli SecA and SecY proteins.Q54567468
A significant fraction of functional SecA is permanently embedded in the membrane. SecA cycling on and off the membrane is not essential during protein translocation.Q54576716
SecA is an intrinsic subunit of the Escherichia coli preprotein translocase and exposes its carboxyl terminus to the periplasm.Q54577867
Domain interactions of the peripheral preprotein Translocase subunit SecA.Q54580709
SecA protein is exposed to the periplasmic surface of the E. coli inner membrane in its active state.Q54627502
SecA, the peripheral subunit of the Escherichia coli precursor protein translocase, is functional as a dimer.Q54646917
SecA, an essential component of the secretory machinery of Escherichia coli, exists as homodimer.Q54701776
Oligomeric Rings of the Sec61p Complex Induced by Ligands Required for Protein TranslocationQ57189552
Separable ATPase and Membrane Insertion Domains of the SecA Subunit of Preprotein TranslocaseQ57976575
Interaction between SecA and SecYEG in Micellar Solution and Formation of the Membrane-Inserted State†Q63359894
Translocase-Bound SecA Is Largely Shielded from the Phospholipid Acyl Chains†Q63359908
SecE-dependent overproduction of SecY in Escherichia coli. Evidence for interaction between two components of the secretory machineryQ68534743
Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocationQ71056385
Reconstitution of an efficient protein translocation machinery comprising SecA and the three membrane proteins, SecY, SecE, and SecG (p12)Q72196641
The aqueous pore through the translocon has a diameter of 40-60 A during cotranslational protein translocation at the ER membraneQ73358906
P433issue5
P407language of work or nameEnglishQ1860
P304page(s)852-861
P577publication date2000-03-01
P1433published inThe EMBO JournalQ1278554
P1476titleSecYEG assembles into a tetramer to form the active protein translocation channel
P478volume19

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cites work (P2860)
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Q44790621Global co-ordination of protein translocation by the SecA IRA1 switch
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