Assembly and overexpression of membrane proteins in Escherichia coli

scientific article

Assembly and overexpression of membrane proteins in Escherichia coli is …
instance of (P31):
scholarly articleQ13442814
review articleQ7318358

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P356DOI10.1016/S0005-2736(02)00707-1
P698PubMed publication ID12586374

P50authorDavid DrewQ56427827
P2093author name stringJan-Willem L de Gier
Louise Baars
Linda Fröderberg
P2860cites workSecA is required for the insertion of inner membrane proteins targeted by the Escherichia coli signal recognition particleQ74620468
Enhancing effect of Bacillus subtilis Ffh, a homologue of the SRP54 subunit of the mammalian signal recognition particle, on the binding of SecA to precursors of secretory proteins in vitroQ77766248
Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factorQ77789106
Targeting and assembly of periplasmic and outer-membrane proteins in Escherichia coliQ77936213
Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organismsQ24673118
BiP acts as a molecular ratchet during posttranslational transport of prepro-alpha factor across the ER membraneQ27932673
Three-dimensional structure of the bacterial protein-translocation complex SecYEGQ28217206
YidC, an assembly site for polytopic Escherichia coli membrane proteins located in immediate proximity to the SecYE translocon and lipidsQ28344574
A novel type of co-chaperone mediates transmembrane recruitment of DnaK-like chaperones to ribosomesQ28588605
Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levelsQ29547813
The Rieske Fe/S protein of the cytochrome b6/f complex in chloroplasts: missing link in the evolution of protein transport pathways in chloroplasts?Q30320929
Membrane proteins: shaping up.Q30330485
SecYEG assembles into a tetramer to form the active protein translocation channelQ30843286
Recombinant protein expression in Escherichia coliQ33744852
Sec-dependent membrane protein insertion: sequential interaction of nascent FtsQ with SecY and YidC.Q33757388
Lipid-assisted protein foldingQ33796621
The Tat protein export pathwayQ33826511
Sec-dependent membrane protein biogenesis: SecYEG, preprotein hydrophobicity and translocation kinetics control the stop-transfer functionQ33888178
Escherichia coli translocase: the unravelling of a molecular machineQ33994549
Physiological basis for conservation of the signal recognition particle targeting pathway in Escherichia coliQ33995882
Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysisQ34014007
Employing Escherichia coli to functionally express, purify, and characterize a human transporterQ34065202
Protein traffic in bacteria: multiple routes from the ribosome to and across the membraneQ34070563
The SecYEG preprotein translocation channel is a conformationally dynamic and dimeric structureQ34086060
A polytopic membrane protein displays a reversible topology dependent on membrane lipid compositionQ34087224
New prospects in studying the bacterial signal recognition particle pathway.Q34109335
Ribosome exchange revisited: a mechanism for translation-coupled ribosome detachment from the ER membraneQ34221158
Biogenesis of inner membrane proteins in Escherichia coliQ34222736
The structural basis of protein targeting and translocation in bacteria.Q34261907
The signal recognition particleQ34275497
Genetic screen yields mutations in genes encoding all known components of the Escherichia coli signal recognition particle pathwayQ34304210
YidC/Oxa1p/Alb3: evolutionarily conserved mediators of membrane protein assemblyQ34310139
Functional analysis of the signal recognition particle in Escherichia coli by characterization of a temperature-sensitive ffh mutantQ34310953
FtsH is required for proteolytic elimination of uncomplexed forms of SecY, an essential protein translocase subunitQ34342148
The Sec protein-translocation pathway.Q34399330
Evaluating the oligomeric state of SecYEG in preprotein translocaseQ34486443
The positive inside rule is not determined by the polarity of the ΔψQ61945158
Is Ffh required for export of secretory proteins?Q63359658
Nascent Lep inserts into the Escherichia coli inner membrane in the vicinity of YidC, SecY and SecAQ63359665
SecA Is Not Required for Signal Recognition Particle-mediated Targeting and Initial Membrane Insertion of a Nascent Inner Membrane ProteinQ63359672
The signal recognition particle-targeting pathway does not necessarily deliver proteins to the sec-translocase in Escherichia coliQ63359674
Characterisation of new intracellular membranes in Escherichia coli accompanying large scale over-production of the b subunit of F(1)F(o) ATP synthaseQ73055713
Chloroplast Oxa1p homolog albino3 is required for post-translational integration of the light harvesting chlorophyll-binding protein into thylakoid membranesQ73357519
Role of Sec61alpha in the regulated transfer of the ribosome-nascent chain complex from the signal recognition particle to the translocation channelQ73453521
Putative integral membrane SRP receptorsQ73624037
The thylakoid delta pH-dependent pathway machinery facilitates RR-independent N-tail protein integrationQ73804955
FtsY binds to the Escherichia coli inner membrane via interactions with phosphatidylethanolamine and membrane proteinsQ73883025
Regulation of ribosome detachment from the mammalian endoplasmic reticulum membraneQ74105312
YidC mediates membrane protein insertion in bacteriaQ74191988
Function of YidC for the insertion of M13 procoat protein in Escherichia coli: translocation of mutants that show differences in their membrane potential dependence and Sec requirementQ74215757
BiP maintains the permeability barrier of the ER membrane by sealing the lumenal end of the translocon pore before and early in translocationQ74405725
A mutation in the Escherichia coli secY gene that produces distinct effects on inner membrane protein insertion and protein exportQ74516874
Mechanisms of subcellular mRNA localizationQ34574332
YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocaseQ34663216
The targeting pathway of Escherichia coli presecretory and integral membrane proteins is specified by the hydrophobicity of the targeting signalQ34741147
Association of Escherichia coli ribosomes with the inner membrane requires the signal recognition particle receptor but is independent of the signal recognition particleQ35688567
A mutant hunt for defects in membrane protein assembly yields mutations affecting the bacterial signal recognition particle and Sec machineryQ35692775
Targeting of GroEL to SecA on the cytoplasmic membrane of Escherichia coliQ35738230
Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coliQ36753051
Early events in preprotein recognition in E. coli: interaction of SRP and trigger factor with nascent polypeptides.Q38289182
Projection structure and oligomeric properties of a bacterial core protein translocaseQ39645201
Evolutionarily conserved binding of ribosomes to the translocation channel via the large ribosomal RNA.Q40370425
Compartmentalization of transcription and translation in Bacillus subtilisQ40387114
SRP-dependent co-translational targeting and SecA-dependent translocation analyzed as individual steps in the export of a bacterial proteinQ40388434
Sec-independent protein insertion into the inner E. coli membrane. A phenomenon in search of an explanationQ40764101
An alternative protein targeting pathway in Escherichia coli: studies on the role of FtsY.Q40791891
The SecA and SecY subunits of translocase are the nearest neighbors of a translocating preprotein, shielding it from phospholipidsQ40871781
Protein transport across the eukaryotic endoplasmic reticulum and bacterial inner membranesQ41114798
Switching the model: a concerted mechanism for GTPases in protein targetingQ41496760
Discrimination between SRP- and SecA/SecB-dependent substrates involves selective recognition of nascent chains by SRP and trigger factorQ41948599
Dissecting the translocase and integrase functions of the Escherichia coli SecYEG transloconQ42077359
Evidence for coupling of membrane targeting and function of the signal recognition particle (SRP) receptor FtsY.Q42211675
The Escherichia coli SRP and SecB targeting pathways converge at the transloconQ42643547
Dislocation of membrane proteins in FtsH-mediated proteolysisQ42674631
Functional reconstitution of purified metabotropic glutamate receptor expressed in the fly eye.Q42724469
Effects of chlorophyll a, chlorophyll b, and xanthophylls on the in vitro assembly kinetics of the major light-harvesting chlorophyll a/b complex, LHCIIb.Q43575286
SecDFyajC is not required for the maintenance of the proton motive forceQ43798449
Direct interaction of YidC with the Sec-independent Pf3 coat protein during its membrane protein insertionQ43829882
YidC and SecY mediate membrane insertion of a Type I transmembrane domain.Q44055090
Messenger RNA targeting of rice seed storage proteins to specific ER subdomainsQ44593670
Analysis of the role of interfacial tryptophan residues in controlling the topology of membrane proteinsQ45099432
Architecture of the protein-conducting channel associated with the translating 80S ribosomeQ49485686
Endoplasmic reticulum-bound ribosomes reside in stable association with the translocon following termination of protein synthesis.Q50336114
Chloroplast YidC homolog Albino3 can functionally complement the bacterial YidC depletion strain and promote membrane insertion of both bacterial and chloroplast thylakoid proteins.Q52122748
The protein-conducting channel in the membrane of the endoplasmic reticulum is open laterally toward the lipid bilayer.Q54011077
SecDFyajC forms a heterotetrameric complex with YidC.Q54543620
Construction of the 'minimal' SRP that interacts with the translating ribosome but not with specific membrane receptors in Escherichia coli.Q54548752
Targeting, insertion, and localization of Escherichia coli YidC.Q54551389
Expression of an olfactory receptor in Escherichia coli: purification, reconstitution, and ligand binding.Q54575193
Chaperonin-promoted post-translational membrane insertion of a multispanning membrane protein lactose permease.Q54580962
The DsbA-DsbB system affects the formation of disulfide bonds in periplasmic but not in intramembraneous protein domains.Q54649845
The Structure of Ribosome-Channel Complexes Engaged in Protein TranslocationQ57189531
Oligomeric Rings of the Sec61p Complex Induced by Ligands Required for Protein TranslocationQ57189552
Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coliQ57381567
Control of topology and mode of assembly of a polytopic membrane protein by positively charged residuesQ57381683
Refolding of Escherichia coli produced membrane protein inclusion bodies immobilised by nickel chelating chromatographyQ57807182
The final stages of folding of the membrane protein bacteriorhodopsin occur by kinetically indistinguishable parallel folding paths that are mediated by pH 1 1Edited by A. R. FershtQ57905244
Translation/Secretion Coupling by Type III Secretion SystemsQ59210021
P433issue1
P407language of work or nameEnglishQ1860
P921main subjectmembrane proteinQ423042
Escherichia coliQ25419
overexpressionQ61643320
P304page(s)3-10
P577publication date2003-02-01
P1433published inBiochimica et Biophysica ActaQ864239
P1476titleAssembly and overexpression of membrane proteins in Escherichia coli
P478volume1610

Reverse relations

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