scholarly article | Q13442814 |
P819 | ADS bibcode | 2008PNAS..105.5722G |
P356 | DOI | 10.1073/PNAS.0802190105 |
P932 | PMC publication ID | 2311375 |
P698 | PubMed publication ID | 18391190 |
P5875 | ResearchGate publication ID | 5461752 |
P50 | author | Eric R Geertsma | Q54437669 |
P2093 | author name string | Bert Poolman | |
Dirk-Jan Slotboom | |||
Maarten Groeneveld | |||
P2860 | cites work | Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter | Q27860697 |
The Membrane Protein Data Bank | Q31021464 | ||
Characteristics affecting expression and solubilization of yeast membrane proteins | Q33262383 | ||
Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants | Q33637017 | ||
Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins | Q34514061 | ||
Assembly and overexpression of membrane proteins in Escherichia coli | Q35066136 | ||
Practical aspects of overexpressing bacterial secondary membrane transporters for structural studies. | Q35066145 | ||
Sec-translocase mediated membrane protein biogenesis | Q35951932 | ||
High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae | Q35956788 | ||
Host/vector interactions which affect the viability of recombinant phage lambda clones | Q36419893 | ||
Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems | Q36514863 | ||
A scalable, GFP-based pipeline for membrane protein overexpression screening and purification | Q36518068 | ||
The activity of the lactose transporter from Streptococcus thermophilus is increased by phosphorylated IIA and the action of beta-galactosidase. | Q38318412 | ||
An efficient strategy for high-throughput expression screening of recombinant integral membrane proteins | Q42020331 | ||
Identification of the Dimer Interface of the Lactose Transport Protein from Streptococcus thermophilus | Q44590116 | ||
Structure of rat monoamine oxidase A and its specific recognitions for substrates and inhibitors | Q44819431 | ||
Optimization of membrane protein overexpression and purification using GFP fusions. | Q50735914 | ||
High-throughput cloning and expression in recalcitrant bacteria. | Q54437626 | ||
Rapid protein-folding assay using green fluorescent protein | Q56902199 | ||
Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli | Q57381567 | ||
Construction of a fusion protein between protein A and green fluorescent protein and its application to western blotting | Q71034663 | ||
Rigidity of the subunit interfaces of the trimeric glutamate transporter GltT during translocation | Q80730310 | ||
P433 | issue | 15 | |
P407 | language of work or name | English | Q1860 |
P921 | main subject | membrane protein | Q423042 |
quality control | Q827792 | ||
overexpression | Q61643320 | ||
P304 | page(s) | 5722-5727 | |
P577 | publication date | 2008-04-07 | |
P1433 | published in | Proceedings of the National Academy of Sciences of the United States of America | Q1146531 |
P1476 | title | Quality control of overexpressed membrane proteins | |
P478 | volume | 105 |
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Q30402414 | A rapid expression and purification condition screening protocol for membrane protein structural biology |
Q49597504 | A statistical model for improved membrane protein expression using sequence-derived features |
Q55438095 | A uniform cloning platform for mycobacterial genetics and protein production. |
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Q41620430 | An agmatine-inducible system for the expression of recombinant proteins in Enterococcus faecalis. |
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Q30425362 | Evaluation of cell-free protein synthesis for the crystallization of membrane proteins--a case study on a member of the glutamate transporter family from Staphylothermus marinus |
Q64976685 | Expression of Leptospira membrane proteins Signal Peptidase (SP) and Leptospira Endostatin like A (Len A) in BL-21(DE3) is toxic to the host cells. |
Q89612908 | Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update |
Q40969055 | Expression, purification, and contaminant detection for structural studies of Ralstonia metallidurance ClC protein rm1. |
Q34359630 | Fluorescent in situ folding control for rapid optimization of cell-free membrane protein synthesis |
Q45845924 | Functional reconstitution and osmoregulatory properties of the ProU ABC transporter from Escherichia coli |
Q30356353 | Functional reconstitution of Staphylococcus aureus truncated AgrC histidine kinase in a model membrane system. |
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Q33373087 | Genetic analysis of G protein-coupled receptor expression in Escherichia coli: inhibitory role of DnaJ on the membrane integration of the human central cannabinoid receptor |
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Q42687931 | Stress-responsive systems set specific limits to the overproduction of membrane proteins in Bacillus subtilis |
Q27702145 | Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family |
Q88423039 | Substrate Insolubility Dictates Hsp104-Dependent Endoplasmic-Reticulum-Associated Degradation |
Q50037931 | The C-terminal domain of the Salmonella enterica WbaP (UDP-galactose:Und-P galactose-1-phosphate transferase) is sufficient for catalytic activity and specificity for undecaprenyl monophosphate |
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Q47288753 | The tunable pReX expression vector enables optimizing the T7-based production of membrane and secretory proteins in E. coli. |
Q34158683 | The tyrosyl-tRNA synthetase like gene located in the tyramine biosynthesis cluster of Enterococcus durans is transcriptionally regulated by tyrosine concentration and extracellular pH. |
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Q35946289 | Three Recombinant Engineered Antibodies against Recombinant Tags with High Affinity and Specificity |
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