| scholarly article | Q13442814 |
| P2093 | author name string | Dick B Janssen | |
| Robert J Floor | |||
| Jan-Metske van der Laan | |||
| Hein J Wijma | |||
| Jan G E van Leeuwen | |||
| P2860 | cites work | Structure-guided recombination creates an artificial family of cytochromes P450. | Q21146061 |
| DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution | Q24562755 | ||
| SCHEMA recombination of a fungal cellulase uncovers a single mutation that contributes markedly to stability | Q24648550 | ||
| Haloalkane dehalogenases: structure of a Rhodococcus enzyme | Q27620589 | ||
| Structure and mechanism of a bacterial haloalcohol dehalogenase: a new variation of the short-chain dehydrogenase/reductase fold without an NAD(P)H binding site | Q27642251 | ||
| Thermostable Bacillus subtilis lipases: in vitro evolution and structural insight | Q27651025 | ||
| Enantioselectivity of haloalkane dehalogenases and its modulation by surface loop engineering | Q27663578 | ||
| Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels | Q27664335 | ||
| Natural diversity to guide focused directed evolution | Q28289669 | ||
| Thermostabilization of an esterase by alignment-guided focussed directed evolution | Q28295698 | ||
| Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes | Q28298658 | ||
| Increasing the precision of comparative models with YASARA NOVA-a self-parameterizing force field | Q28874041 | ||
| AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility | Q29547658 | ||
| Thermofluor-based high-throughput stability optimization of proteins for structural studies | Q29614747 | ||
| HotSpot Wizard: a web server for identification of hot spots in protein engineering. | Q30377457 | ||
| Directed evolution of enzymes for applied biocatalysis | Q30823434 | ||
| Directed evolution study of temperature adaptation in a psychrophilic enzyme | Q30851002 | ||
| User-friendly algorithms for estimating completeness and diversity in randomized protein-encoding libraries | Q30967215 | ||
| Better library design: data-driven protein engineering | Q31087378 | ||
| Novel methods for directed evolution of enzymes: quality, not quantity | Q31105264 | ||
| Efficient screening of fungal cellobiohydrolase class I enzymes for thermostabilizing sequence blocks by SCHEMA structure-guided recombination | Q39111595 | ||
| Laboratory evolution of toluene dioxygenase to accept 4-picoline as a substrate | Q39492303 | ||
| Haloalkane-utilizing Rhodococcus strains isolated from geographically distinct locations possess a highly conserved gene cluster encoding haloalkane catabolism | Q39539197 | ||
| Roles of horizontal gene transfer and gene integration in evolution of 1,3-dichloropropene- and 1,2-dibromoethane-degradative pathways | Q39587105 | ||
| Site-saturation mutagenesis of tryptophan 116 of Saccharomyces pastorianus old yellow enzyme uncovers stereocomplementary variants | Q40001594 | ||
| Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution | Q40695874 | ||
| Manipulating the Stereoselectivity of Limonene Epoxide Hydrolase by Directed Evolution Based on Iterative Saturation Mutagenesis | Q42854484 | ||
| Structurally Informed Site-Directed Mutagenesis of a Stereochemically Promiscuous Aldolase To Afford Stereochemically Complementary Biocatalysts | Q42946960 | ||
| Directed evolution of an antitumor drug (arginine deiminase PpADI) for increased activity at physiological pH. | Q43161107 | ||
| Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fluorescens esterase towards methyl 3bromo-2-methylpropanoate and ethyl 3phenylbutyrate | Q44436779 | ||
| Laboratory evolution of cytochrome p450 BM-3 monooxygenase for organic cosolvents | Q44745675 | ||
| Directed evolution of enantioselective enzymes: an unconventional approach to asymmetric catalysis in organic chemistry | Q45083234 | ||
| Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations | Q45233927 | ||
| Loop grafting of Bacillus subtilis lipase A: inversion of enantioselectivity. | Q46415327 | ||
| Strategies for the in vitro evolution of protein function: enzyme evolution by random recombination of improved sequences | Q46855416 | ||
| Improving catalytic function by ProSAR-driven enzyme evolution | Q46928936 | ||
| Engineering endoglucanase II from Trichoderma reesei to improve the catalytic efficiency at a higher pH optimum | Q47717511 | ||
| The plasmid-located haloalkane dehalogenase gene from Rhodococcus rhodochrous NCIMB 13064. | Q48056123 | ||
| Phylogenetic analysis of haloalkane dehalogenases | Q48414049 | ||
| Indicator media for microorganisms degrading chlorinated pesticides | Q50961432 | ||
| Structure-guided consensus approach to create a more thermostable penicillin G acylase | Q51150471 | ||
| A novel solid phase technology for high-throughput gene synthesis. | Q51869754 | ||
| Rational evolutionary design: The theory of in vitro protein evolution | Q56690317 | ||
| Creation of an amino acid network of structurally coupled residues in the directed evolution of a thermostable enzyme | Q56897572 | ||
| Directed evolution of a Baeyer-Villiger monooxygenase to enhance enantioselectivity | Q56897621 | ||
| Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions | Q56897652 | ||
| Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability | Q56898721 | ||
| Designing new Baeyer-Villiger monooxygenases using restricted CASTing | Q56898762 | ||
| Directed evolution of an esterase from Pseudomonas fluorescens yields a mutant with excellent enantioselectivity and activity for the kinetic resolution of a chiral building block | Q56899102 | ||
| Directed evolution of cyclohexanone monooxygenases: enantioselective biocatalysts for the oxidation of prochiral thioethers | Q56899921 | ||
| Finding better protein engineering strategies | Q57189348 | ||
| Assembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution | Q31123994 | ||
| Optimising enzyme function by directed evolution. | Q31155398 | ||
| Automated design of degenerate codon libraries | Q33225634 | ||
| Enantioselective epoxidation of terminal alkenes to (R)- and (S)-epoxides by engineered cytochromes P450 BM-3. | Q33225680 | ||
| Expanding the substrate scope of enzymes: combining mutations obtained by CASTing | Q33247423 | ||
| Transversion-enriched sequence saturation mutagenesis (SeSaM-Tv+): a random mutagenesis method with consecutive nucleotide exchanges that complements the bias of error-prone PCR. | Q33306227 | ||
| Amino acid alphabet size in protein evolution experiments: better to search a small library thoroughly or a large library sparsely? | Q33326227 | ||
| Addressing the numbers problem in directed evolution | Q33345289 | ||
| Knowledge-guided laboratory evolution of protein thermolability | Q33392056 | ||
| Enzyme engineering for enantioselectivity: from trial-and-error to rational design? | Q33515950 | ||
| Consensus protein design without phylogenetic bias | Q33569414 | ||
| Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst | Q33758053 | ||
| Bacterial degradation of xenobiotic compounds: evolution and distribution of novel enzyme activities | Q33991671 | ||
| Biodegradation of 1,2,3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene | Q34101277 | ||
| Iterative saturation mutagenesis accelerates laboratory evolution of enzyme stereoselectivity: rigorous comparison with traditional methods. | Q34120009 | ||
| Combining computational and experimental screening for rapid optimization of protein properties | Q34414821 | ||
| Pharmacological profile of the "triple" monoamine neurotransmitter uptake inhibitor, DOV 102,677. | Q34517414 | ||
| Improving physical realism, stereochemistry, and side-chain accuracy in homology modeling: Four approaches that performed well in CASP8. | Q34612025 | ||
| Constructing and analyzing the fitness landscape of an experimental evolutionary process | Q34810511 | ||
| Redesigning dehalogenase access tunnels as a strategy for degrading an anthropogenic substrate | Q34999009 | ||
| Evolving haloalkane dehalogenases | Q35737586 | ||
| Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening | Q36137488 | ||
| Evolving strategies for enzyme engineering | Q36188225 | ||
| Directed evolution of a thermostable esterase. | Q36611228 | ||
| Directed enzyme evolution: climbing fitness peaks one amino acid at a time | Q37244268 | ||
| Enantiocomplementary enzymes: classification, molecular basis for their enantiopreference, and prospects for mirror-image biotransformations. | Q37292553 | ||
| Directed evolution: new parts and optimized function | Q37416828 | ||
| Controlling the enantioselectivity of enzymes by directed evolution: practical and theoretical ramifications | Q37648687 | ||
| Protein engineering of microbial enzymes | Q37695466 | ||
| The alpha/beta-hydrolase fold 3DM database (ABHDB) as a tool for protein engineering | Q37769173 | ||
| Laboratory evolution of stereoselective enzymes: a prolific source of catalysts for asymmetric reactions | Q37780497 | ||
| Learning from directed evolution: Further lessons from theoretical investigations into cooperative mutations in lipase enantioselectivity | Q38307242 | ||
| Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents | Q38359408 | ||
| P433 | issue | 1 | |
| P921 | main subject | directed evolution | Q440681 |
| P304 | page(s) | 137-148 | |
| P577 | publication date | 2011-11-23 | |
| P1433 | published in | ChemBioChem | Q634395 |
| P1476 | title | Directed evolution strategies for enantiocomplementary haloalkane dehalogenases: from chemical waste to enantiopure building blocks | |
| P478 | volume | 13 |
| Q35638773 | A selection assay for haloalkane dehalogenase activity based on toxic substrates |
| Q35362816 | Characterization of a recombinant (+)-γ-lactamase from Microbacterium hydrocarbonoxydans which provides evidence that two enantiocomplementary γ-lactamases are in the strain |
| Q41204500 | Combinatorial metabolic engineering of Pseudomonas putida KT2440 for efficient mineralization of 1,2,3-trichloropropane |
| Q35197299 | Computational library design for increasing haloalkane dehalogenase stability |
| Q48368998 | Different Structural Origins of the Enantioselectivity of Haloalkane Dehalogenases toward Linear β-Haloalkanes: Open-Solvated versus Occluded-Desolvated Active Sites. |
| Q35087224 | Draft genome sequence of Rhodococcus rhodochrous strain ATCC 17895 |
| Q35221137 | Exploring the enantioselective mechanism of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by iterative saturation mutagenesis |
| Q38042407 | Haloalkane dehalogenases: biotechnological applications. |
| Q27671016 | Hydroxynitrile Lyases with α/β-Hydrolase Fold: Two Enzymes with Almost Identical 3D Structures but Opposite Enantioselectivities and Different Reaction Mechanisms |
| Q35196065 | Improvement of biocatalysts for industrial and environmental purposes by saturation mutagenesis |
| Q38117861 | Inverting hydrolases and their use in enantioconvergent biotransformations |
| Q35182675 | Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations |
| Q51061048 | Maximizing the efficiency of multienzyme process by stoichiometry optimization. |
| Q38172428 | Microbial alkyl- and aryl-sulfatases: mechanism, occurrence, screening and stereoselectivities |
| Q38587501 | Properties and biotechnological applications of natural and engineered haloalkane dehalogenases |
| Q47215183 | Protein engineering of oxidoreductases utilizing nicotinamide-based coenzymes, with applications in synthetic biology. |
| Q92644514 | Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance |
| Q38287400 | Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently |
| Q36150163 | Transformation and biodegradation of 1,2,3-trichloropropane (TCP). |
Search more.