Diversification and specialization of HIV protease function during in vitro evolution

scientific article

Diversification and specialization of HIV protease function during in vitro evolution is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1093/MOLBEV/MSJ098
P932PMC publication ID2012945
P698PubMed publication ID16423863
P5875ResearchGate publication ID7348622

P2093author name stringIchiro Matsumura
Dina N Greene
Taryn L O'Loughlin
P2860cites workConformational diversity and protein evolution--a 60-year-old hypothesis revisitedQ73702393
Cell killing by HIV-1 proteaseQ78346287
Active human immunodeficiency virus protease is required for viral infectivityQ24647696
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingQ25938984
How does a symmetric dimer recognize an asymmetric substrate? A substrate complex of HIV-1 proteaseQ27626817
Substrate shape determines specificity of recognition for HIV-1 protease: analysis of crystal structures of six substrate complexesQ27638967
The structure of tumor necrosis factor-alpha at 2.6 A resolution. Implications for receptor bindingQ27696873
Human immunodeficiency virus protease ligand specificity conferred by residues outside of the active site cavityQ27733298
Statistical methods for detecting molecular adaptationQ28140163
In vitro evolution of beta-glucuronidase into a beta-galactosidase proceeds through non-specific intermediatesQ28140815
Rapid evolution of a protein in vitro by DNA shufflingQ28245873
The 'evolvability' of promiscuous protein functionsQ28295483
Novel fluorogenic substrates for assaying retroviral proteases by resonance energy transferQ28329194
High efficiency transformation of E. coli by high voltage electroporationQ29615278
A perspective on enzyme catalysis.Q30333357
The structural basis of molecular adaptationQ30430193
Randomization of genes by PCR mutagenesisQ33198019
Rapid evolution of beta-glucuronidase specificity by saturation mutagenesis of an active site loopQ33201151
Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity.Q33214630
Isolation of mutants of human immunodeficiency virus protease based on the toxicity of the enzyme in Escherichia coliQ33703756
beta-Galactosidase containing a human immunodeficiency virus protease cleavage site is cleaved and inactivated by human immunodeficiency virus proteaseQ33927954
Resurrecting ancient genes: experimental analysis of extinct moleculesQ33978298
Recombination studies of lactose nonfermenting mutants of Escherichia coli K-12Q33979024
Adaptive evolution of genes and gene familiesQ35592999
Directed evolution of an aspartate aminotransferase with new substrate specificitiesQ36089937
Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solventsQ38359408
Cachectin/tumor necrosis factor: an endogenous mediator of shock and inflammationQ39509936
HIV-1 protease variants from 100-fold drug resistant clinical isolates: expression, purification, and crystallizationQ44371788
P433issue4
P304page(s)764-772
P577publication date2006-01-19
P1433published inMolecular Biology and EvolutionQ1992656
P1476titleDiversification and specialization of HIV protease function during in vitro evolution
P478volume23

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cites work (P2860)
Q21559609A tobacco etch virus protease with increased substrate tolerance at the P1' position
Q64376468An Adaptable Platform for Directed Evolution in Human Cells
Q24273262Computational enzyme design approaches with significant biological outcomes: progress and challenges
Q35034759Directed evolution of aminoglycoside phosphotransferase (3') type IIIa variants that inactivate amikacin but impose significant fitness costs
Q35967157Directed evolution of highly selective proteases by using a novel FACS-based screen that capitalizes on the p53 regulator MDM2
Q33254710Directed evolution: an approach to engineer enzymes
Q38833850Emerging technologies for protease engineering: New tools to clear out disease
Q33497078Engineering next generation proteases
Q24630061Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries
Q28755510Evolutionary history of a specialized p450 propane monooxygenase
Q40838288Fluorometric assay for phenotypic differentiation of drug-resistant HIV mutants.
Q36439810Gene functional trade-offs and the evolution of pleiotropy.
Q28257999Intracellular detection and evolution of site-specific proteases using a genetic selection system
Q30479736Neutral genetic drift can alter promiscuous protein functions, potentially aiding functional evolution
Q38098276Reporter-based screening and selection of enzymes
Q41051814Simultaneous selection and counter-selection for the directed evolution of proteases in E. coli using a cytoplasmic anchoring strategy
Q38009054The proteomic future: where mass spectrometry should be taking us.

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