Engineering anti-vascular endothelial growth factor single chain disulfide-stabilized antibody variable fragments (sc-dsFv) with phage-displayed sc-dsFv libraries

scientific article

Engineering anti-vascular endothelial growth factor single chain disulfide-stabilized antibody variable fragments (sc-dsFv) with phage-displayed sc-dsFv libraries is …
instance of (P31):
scholarly articleQ13442814

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P356DOI10.1074/JBC.M109.061457
P932PMC publication ID2832938
P698PubMed publication ID20068035
P5875ResearchGate publication ID41000705

P50authorAn-Suei YangQ56999385
P2093author name stringYi-Jen Huang
Anna Tung Ching Ching
Chung-Ming Yu
Hung-Ju Chang
Hung-Ju Hsu
Ing-Chien Chen
Yu-Ching Lee
P2860cites workDNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesisQ24529935
Structure of a complex of the ATPase SecA and the protein-translocation channelQ27652526
Conformational transition of Sec machinery inferred from bacterial SecYE structuresQ27652527
Rapid and efficient site-specific mutagenesis without phenotypic selectionQ27860628
Selection of phage antibodies by binding affinity. Mimicking affinity maturationQ28255489
Locating proteins in the cell using TargetP, SignalP and related toolsQ29547300
Assessing computational amino acid beta-turn propensities with a phage-displayed combinatorial library and directed evolutionQ30827438
Stability engineering of antibody single-chain Fv fragmentsQ30978689
By-passing immunization: building high affinity human antibodies by chain shufflingQ30988323
Improving the display of proteins on filamentous phageQ31908651
Antibodies from phage antibody librariesQ33204934
Selecting and screening recombinant antibody librariesQ33223036
Structure-function studies of two synthetic anti-vascular endothelial growth factor Fabs and comparison with the Avastin Fab.Q33230191
Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage displayQ33249034
Submitting antibodies to binding arbitrationQ33335707
SRP and Sec pathway leader peptides for antibody phage display and antibody fragment production in E. coliQ33337825
Use of synthetic signal sequences to explore the protein export machineryQ33344714
Protease substrate site predictors derived from machine learning on multilevel substrate phage display dataQ33381460
A surface expression vector for antibody screeningQ33402114
Molecular evolution of cystine-stabilized miniproteins as stable proteinaceous bindersQ33430565
Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coliQ33638891
Phage antibodies: filamentous phage displaying antibody variable domainsQ34031345
SRP-mediated protein targeting: structure and function revisitedQ34368252
Engineered antibody fragments and the rise of single domainsQ34650980
The filamentous bacteriophage assembly proteins require the bacterial SecA protein for correct localization to the membraneQ36087935
Potent antibody therapeutics by designQ36452621
Latest technologies for the enhancement of antibody affinityQ36530976
The surprising complexity of signal sequencesQ36571413
Phage display of disulfide-stabilized Fv fragmentsQ36706101
Factor Xa active site substrate specificity with substrate phage display and computational molecular modeling.Q36727212
Protein translocation across the bacterial cytoplasmic membrane.Q37031528
Disulfide stabilization of antibody Fv: computer predictions and experimental evaluationQ38288220
Single-chain Fvs.Q40569728
Assembly of a functional immunoglobulin Fv fragment in Escherichia coliQ41278018
Single chain Fab (scFab) fragmentQ41982040
Signal sequences control gating of the protein translocation channel in a substrate-specific mannerQ42818885
Domain interactions in the Fab fragment: a comparative evaluation of the single-chain Fv and Fab format engineered with variable domains of different stabilityQ46380702
Synthetic human antibodies and a strategy for protein engineeringQ47789160
Engineering antibody Fv fragments for cancer detection and therapy: Bisulfide-stabilized Fv fragmentsQ47960256
Thermal stabilization of a single-chain Fv antibody fragment by introduction of a disulphide bondQ50141295
Signal sequences: more than just greasy peptidesQ56639128
Selection of phage antibodies by binding affinityQ56690318
Different Equilibrium Stability Behavior of ScFv Fragments: Identification, Classification, and Improvement by Protein Engineering†Q57840032
Rapid tumor penetration of a single-chain Fv and comparison with other immunoglobulin formsQ68141138
Single-chain antigen-binding proteinsQ70406917
A form of anti-Tac(Fv) which is both single-chain and disulfide stabilized: comparison with its single-chain and disulfide-stabilized homologsQ74445753
P433issue11
P407language of work or nameEnglishQ1860
P921main subjectendotheliumQ111140
P304page(s)7880-7891
P577publication date2010-01-12
P1433published inJournal of Biological ChemistryQ867727
P1476titleEngineering anti-vascular endothelial growth factor single chain disulfide-stabilized antibody variable fragments (sc-dsFv) with phage-displayed sc-dsFv libraries
P478volume285

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cites work (P2860)
Q34323991Antibody phage display libraries: contributions to oncology.
Q34010654Expression, production, and renaturation of a functional single-chain variable antibody fragment (scFv) against human ICAM-1
Q39017303Identification of two aberrant transcripts derived from a hybridoma with amplification of functional immunoglobulin variable genes
Q30155518Probing the stability-limiting regions of an antibody single-chain variable fragment: a molecular dynamics simulation study
Q27678251Rationalization and Design of the Complementarity Determining Region Sequences in an Antibody-Antigen Recognition Interface
Q34323205Synthetic antibodies: Concepts, potential and practical considerations
Q47763614Towards a universal disulphide stabilised single chain Fv format: importance of interchain disulphide bond location and vL-vH orientation

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