Variable region framework differences result in decreased or increased affinity of variant anti-digoxin antibodies

scientific article

Variable region framework differences result in decreased or increased affinity of variant anti-digoxin antibodies is …
instance of (P31):
scholarly articleQ13442814

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P819ADS bibcode1988PNAS...85.3080P
P356DOI10.1073/PNAS.85.9.3080
P932PMC publication ID280147
P698PubMed publication ID3129726
P5875ResearchGate publication ID20026014

P50authorMeredith Mudgett-HunterQ105036024
P2093author name stringL A Herzenberg
M N Margolies
E Haber
L L Peterson
D R Parks
D J Panka
P2860cites workContinuous cultures of fused cells secreting antibody of predefined specificityQ26776979
Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseQ26778460
Phosphocholine binding immunoglobulin Fab McPC603. An X-ray diffraction study at 2.7 AQ27728519
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Somatic generation of antibody diversityQ29616439
Patterns of amino acids near signal-sequence cleavage sitesQ29616542
Origin of immune diversity: genetic variation and selectionQ34195528
Variability of three-dimensional structure in immunoglobulinsQ35071741
An analysis of the sequences of the variable regions of Bence Jones proteins and myeloma light chains and their implications for antibody complementarityQ36270017
Somatic mutation in a cultured mouse myeloma cell affects antigen bindingQ36283076
Single amino acid substitution altering antigen-binding specificityQ36287596
Variable region sequences of murine IgM anti-IgG monoclonal autoantibodies (rheumatoid factors). A structural explanation for the high frequency of IgM anti-IgG B cellsQ36352229
Myxobacter AL-1 protease II: specific peptide bond cleavage on the amino side of lysine.Q36834929
Subcellular localization and levels of aminopeptidases and dipeptidase in Saccharomyces cerevisiaeQ40964248
Immunoglobulin V region variants in hybridoma cells. I. Isolation of a variant with altered idiotypic and antigen binding specificityQ41305386
Immunoglobulin V region variants in hybridoma cells. II. Recombination between V genes.Q41305508
Complete variable region sequences of five homologous high affinity anti-digoxin antibodiesQ48337399
Complete amino acid sequences of the heavy and light chain variable regions from two A/J mouse antigen nonbinding monoclonal antibodies bearing the predominant p-azophenyl arsonate idiotypeQ48350513
Proteolysis in eucaryotic cells: Aminopeptidases and dipeptidyl aminopeptidases of yeast revisitedQ53049093
Binding and structural diversity among high-affinity monoclonal anti-digoxin antibodies.Q54190943
How signal sequences maintain cleavage specificityQ57381692
Preliminary refinement and structural analysis of the Fab fragment from human immunoglobulin new at 2.0 A resolutionQ67243959
Characterization of antibodies of high affinity and specificity for the digitalis glycoside digoxinQ68605003
The galactan-binding immunoglobulin Fab J539: an X-ray diffraction study at 2.6-A resolutionQ68704276
Three-dimensional structure of an antigen-antibody complex at 2.8 A resolutionQ68969863
Drastic change in idiotypic but not antigen-binding specificity of an antibody by a single amino-acid substitutionQ69383889
Immunoglobulin chain recombination among antidigoxin antibodies by hybridoma-hybridoma fusionQ69401421
Use of o-phthalaldehyde to reduce background during automated Edman degradationQ70483021
Complete amino acid sequence of the heavy-chain variable region from an A/J mouse antigen-nonbinding monoclonal antibody bearing the predominant arsonate idiotypeQ70505179
Crystallographic refinement and atomic models of the intact immunoglobulin molecule Kol and its antigen-binding fragment at 3.0 A and 1.0 A resolutionQ71405857
High-affinity monoclonal antibodies to the cardiac glycoside, digoxinQ72673864
P433issue9
P407language of work or nameEnglishQ1860
P921main subjectantibodyQ79460
P1104number of pages5
P304page(s)3080-3084
P577publication date1988-05-01
P1433published inProceedings of the National Academy of Sciences of the United States of AmericaQ1146531
P1476titleVariable region framework differences result in decreased or increased affinity of variant anti-digoxin antibodies
P478volume85

Reverse relations

described by source (P1343)
Q116048837Dig 40-150
Q116048838Dig 40-150 A2.4
Q116048839Dig 40-150 A2.4 P.10

cites work (P2860)
Q34566178Anti-digoxin Fab variants generated by phage display
Q37820247Antibody engineering toward high-sensitivity high-throughput immunosensing of small molecules
Q48142878Characterization of two human anti-DNA antibodies bearing the pathogenic idiotype 8.12.
Q36278518Contribution of a single heavy chain residue to specificity of an anti-digoxin monoclonal antibody
Q33993598Determination of antibody affinity
Q40945142Efficient amplification and direct sequencing of mouse variable regions from any immunoglobulin gene family
Q35180023Engineered Antibodies as Pharmacological Tools
Q32033280Filamentous bacteriophage display of a bifunctional protein A::scFv fusion
Q44795819Human monoclonal antibodies with different fine specificity for digoxin derivatives: cloning of heavy and light chain variable region sequences
Q31131763Immunoglobulin heavy chain gene expression in peripheral blood B lymphocytes
Q36377306Influence of the NH2-terminal amino acid of the T cell receptor alpha chain on major histocompatibility complex (MHC) class II + peptide recognition
Q36277890Modulation of antibody affinity by a non-contact residue
Q48150512Molecular analysis of anti-idiotypic monoclonal antibodies in the HLA-DR antigenic system
Q69376859Mouse Vk gene classification by nucleic acid sequence similarity
Q36363653Mutational analysis of an autoantibody: differential binding and pathogenicity
Q33843281Paracrine inhibition of prion propagation by anti-PrP single-chain Fv miniantibodies
Q33638891Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli
Q34296709Protein evolution on rugged landscapes
Q52035304Repertoire shift occurs during the memory maintenance phase of the immune responses and is not affinity-driven.
Q69259730Rheumatoid factor autoantibody-binding site: a molecular analysis using monoclonal antibodies with dual anti-TNP and anti-IgG activities
Q38342345Site-specific mutagenesis of a recombinant anti-single-stranded DNA Fab. Role of heavy chain complementarity-determining region 3 residues in antigen interaction

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