Recombinant expression, purification, and functional characterisation of connective tissue growth factor and nephroblastoma-overexpressed protein

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Recombinant expression, purification, and functional characterisation of connective tissue growth factor and nephroblastoma-overexpressed protein is …
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scholarly articleQ13442814

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P819ADS bibcode2010PLoSO...516000B
P356DOI10.1371/JOURNAL.PONE.0016000
P932PMC publication ID3012735
P698PubMed publication ID21209863
P5875ResearchGate publication ID49732281

P50authorRalf WeiskirchenQ51753160
P2093author name stringKurt Hoffmann
Michael Kupper
Wilhelm Bohr
P2860cites workMASCOT: multiple alignment system for protein sequences based on three-way dynamic programming.Q52398029
Highly Sensitive and Fast Protein Detection with Coomassie Brilliant Blue in Sodium Dodecyl Sulfate-Polyacrylamide Gel ElectrophoresisQ56675193
Differential effects of TGF-β on connective tissue growth factor (CTGF/CCN2) expression in hepatic stellate cells and hepatocytesQ62735258
CCN3 is a novel endogenous PDGF-regulated inhibitor of glomerular cell proliferationQ62805959
The genetics of disulfide bond metabolismQ77936221
Connective tissue growth factor-(CTGF, CCN2)--a marker, mediator and therapeutic target for renal fibrosisQ84972647
Tumor necrosis factor alpha suppresses the induction of connective tissue growth factor by transforming growth factor-beta in normal and scleroderma fibroblastsQ22254093
CCN3 (NOV) Is a Novel Angiogenic Regulator of the CCN Protein FamilyQ24299868
Integrin-dependent functions of the angiogenic inducer NOV (CCN3): implication in wound healingQ24324036
Inhibition of glioma cell growth and tumorigenic potential by CCN3 (NOV)Q24535398
Modern pathogenetic concepts of liver fibrosis suggest stellate cells and TGF-beta as major players and therapeutic targetsQ28303698
NOV (nephroblastoma overexpressed) and the CCN family of genes: structural and functional issuesQ28363601
CCN3 (NOV) is a negative regulator of CCN2 (CTGF) and a novel endogenous inhibitor of the fibrotic pathway in an in vitro model of renal diseaseQ28568759
Direct binding of Smad3 and Smad4 to critical TGF beta-inducible elements in the promoter of human plasminogen activator inhibitor-type 1 geneQ29616540
TGF-beta signaling and the fibrotic responseQ29620099
Matricellular protein CCN3 (NOV) regulates actin cytoskeleton reorganizationQ30492065
Proviral Rearrangements and Overexpression of a New Cellular Gene (nov) in Myeloblastosis-Associated Virus Type 1-Induced NephroblastomasQ31159804
Connective tissue growth factor: a cysteine-rich mitogen secreted by human vascular endothelial cells is related to the SRC-induced immediate early gene product CEF-10Q33236833
The connective tissue growth factor/cysteine-rich 61/nephroblastoma overexpressed (CCN) familyQ33594510
Role of N-linked glycosylation in biosynthesis, trafficking, and function of the human glucagon-like peptide 1 receptorQ33994409
The modular architecture of a new family of growth regulators related to connective tissue growth factorQ34306420
Regulation of connective tissue growth factor gene expression in human skin fibroblasts and during wound repairQ34437758
CCN proteins: multifunctional signalling regulatorsQ35629272
Evolving concepts of liver fibrogenesis provide new diagnostic and therapeutic optionsQ36014644
Molecular requirements for induction of CTGF expression by TGF-beta1 in primary osteoblastsQ36724904
Induction of nephroblastoma by myeloblastosis-associated virus type 1: state of proviral DNAs in tumor cellsQ36792180
Susceptibility to liver fibrosis in mice expressing a connective tissue growth factor transgene in hepatocytesQ37331302
Growth factors and cytokines in wound healingQ37364066
Permanent cell line expressing human factor VIII-related antigen established by hybridizationQ37615209
CCN proteins in normal and injured liverQ37825244
Connective tissue growth factor does not affect transforming growth factor-beta 1-induced Smad3 phosphorylation and T lymphocyte proliferation inhibitionQ39974642
TGFbeta stimulated re-epithelialisation is regulated by CTGF and Ras/MEK/ERK signallingQ40072195
Individual domains of connective tissue growth factor regulate fibroblast proliferation and myofibroblast differentiationQ40429853
The control of ccn2 (ctgf) gene expression in normal and scleroderma fibroblastsQ41766529
Pericytes display increased CCN2 expression upon culturingQ41981273
CTGF expression is induced by TGF- beta in cardiac fibroblasts and cardiac myocytes: a potential role in heart fibrosisQ42494057
CTGF and SMADs, maintenance of scleroderma phenotype is independent of SMAD signalingQ42498294
Connective tissue growth factor (CTGF/CCN2) in hepatic fibrosisQ42600836
Validation of connective tissue growth factor (CTGF/CCN2) and its gene polymorphisms as noninvasive biomarkers for the assessment of liver fibrosisQ45076019
Molecular insights into connective tissue growth factor action in rat pancreatic stellate cellsQ46474579
Inhibitory effect of soluble PDGF-beta receptor in culture-activated hepatic stellate cells.Q47821684
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue12
P407language of work or nameEnglishQ1860
P921main subjectconnective tissueQ25615
overexpressionQ61643320
P304page(s)e16000
P577publication date2010-12-30
P1433published inPLOS OneQ564954
P1476titleRecombinant expression, purification, and functional characterisation of connective tissue growth factor and nephroblastoma-overexpressed protein
P478volume5

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cites work (P2860)
Q36662688CCN2 exerts direct cytoprotective actions in adult cardiac myocytes by activation of the PI3-kinase/Akt/GSK-3β signaling pathway.
Q39508427CCN3/NOV small interfering RNA enhances fibrogenic gene expression in primary hepatic stellate cells and cirrhotic fat storing cell line CFSC.
Q35922588CTGF/CCN2 Postconditioning Increases Tolerance of Murine Hearts towards Ischemia-Reperfusion Injury
Q91825046Connective tissue growth factor (CCN2) is a matricellular preproprotein controlled by proteolytic activation
Q39501756Impact of CCN3 (NOV) glycosylation on migration/invasion properties and cell growth of the choriocarcinoma cell line Jeg3.
Q36850768Pasteurella multocida toxin (PMT) upregulates CTGF which leads to mTORC1 activation in Swiss 3T3 cells

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