SensiScreen®KRAS exon 2-sensitive simplex and multiplex real-time PCR-based assays for detection of KRAS exon 2 mutations

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SensiScreen®KRAS exon 2-sensitive simplex and multiplex real-time PCR-based assays for detection of KRAS exon 2 mutations is …
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P819ADS bibcode2017PLoSO..1278027R
P356DOI10.1371/JOURNAL.PONE.0178027
P932PMC publication ID5479524
P698PubMed publication ID28636636

P50authorMilo FrattiniQ95979739
Stephen Hamilton-DutoitQ42323196
P2093author name stringAlice Riva
Elisabetta Merlo
Christina Andersen
Jan Lorenzen
Samantha Epistolio
Kirsten Voogd
Majbritt Hauge Kyneb
Mariann Guldmann-Christensen
Michael BØrgesen
Tine Yding Wolff
Ulf Bech Christensen
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p16(INK4A) Hypermethylation detected by fluorescent methylation-specific PCR in plasmas from non-small cell lung cancerQ78632829
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Molecular phenotypes of colorectal cancer and potential clinical applicationsQ26785725
Quantitative and sensitive detection of rare mutations using droplet-based microfluidicsQ28238121
The molecular evolution of acquired resistance to targeted EGFR blockade in colorectal cancersQ29395525
Comparison of Sanger sequencing, pyrosequencing, and melting curve analysis for the detection of KRAS mutations: diagnostic and clinical implicationsQ33947050
Ras and Rho GTPases: a family reunionQ34073399
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Intercalating nucleic acids containing insertions of 1-O-(1-pyrenylmethyl)glycerol: stabilisation of dsDNA and discrimination of DNA over RNA.Q34369300
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Validation of targeted next-generation sequencing for RAS mutation detection in FFPE colorectal cancer tissues: comparison with Sanger sequencing and ARMS-Scorpion real-time PCR.Q36472281
A comparison of Direct sequencing, Pyrosequencing, High resolution melting analysis, TheraScreen DxS, and the K-ras StripAssay for detecting KRAS mutations in non small cell lung carcinomasQ36523724
KRAS mutation testing for predicting response to anti-EGFR therapy for colorectal carcinoma: proposal for an European quality assurance program.Q37273361
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Important molecular genetic markers of colorectal cancer.Q37619856
454 next generation-sequencing outperforms allele-specific PCR, Sanger sequencing, and pyrosequencing for routine KRAS mutation analysis of formalin-fixed, paraffin-embedded samples.Q38395259
Spectrum of Gene Mutations in Colorectal Cancer: Implications for TreatmentQ38876058
Mutation spectra of RAS gene family in colorectal cancerQ38890904
Improving the limit of detection for Sanger sequencing: A comparison of methodologies for KRAS variant detectionQ39256278
Clinical relevance of KRAS-mutated subclones detected with picodroplet digital PCR in advanced colorectal cancer treated with anti-EGFR therapyQ40224084
Recurrent KRAS codon 146 mutations in human colorectal cancerQ40232898
Detection of K-ras mutations in stools of patients with colorectal cancer by mutant-enriched PCR.Q41199696
Rapid and sensitive nonradioactive detection of mutant K-ras genes via 'enriched' PCR amplificationQ41682735
Different types of K-Ras mutations are conversely associated with overall survival in patients with colorectal cancer.Q45908649
PTEN expression and KRAS mutations on primary tumors and metastases in the prediction of benefit from cetuximab plus irinotecan for patients with metastatic colorectal cancer.Q46034442
Mutations of ras genes distinguish a subset of non-small-cell lung cancer cell lines from small-cell lung cancer cell linesQ46937946
APC mutations and other genetic and epigenetic changes in colon cancer.Q54446969
Increased detection sensitivity for KRAS mutations enhances the prediction of anti-EGFR monoclonal antibody resistance in metastatic colorectal cancer.Q54585853
P275copyright licenseCreative Commons Attribution 4.0 InternationalQ20007257
P6216copyright statuscopyrightedQ50423863
P433issue6
P407language of work or nameEnglishQ1860
P921main subjectscreen timeQ28130149
P304page(s)e0178027
P577publication date2017-06-21
P1433published inPLOS OneQ564954
P1476titleSensiScreen®KRAS exon 2-sensitive simplex and multiplex real-time PCR-based assays for detection of KRAS exon 2 mutations
P478volume12

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Q64055558Application of lab-on-a-chip multiplex cassette PCR for the detection of enterohemorrhagic Escherichia colicites workP2860

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